A novel small-molecule fluorescent probe caused by minimal structural modifications for specific staining of the cell nuclear membrane.

The nuclear membrane is a double-layered structure that physically protects the cell's DNA from the chemical reactions occurring in other parts of the cell. In this study, we present the first brand-new small-molecule fluorescent probe that selectively stains the nuclear membrane, allowing for the visualization of nuclear morphology without interfering with the DNA's activity.

Investigation of foot care knowledge and behaviour of older people with type 2 diabetes in Beijing community and analysis of influencing factors.

Good foot care knowledge and behaviour are very important to prevent the occurrence of diabetic foot, but there are few reports on the foot care knowledge and behaviour of older people with diabetes in the community. The purpose of this study was to understand the foot care knowledge and behaviour of older people with type 2 diabetes in Beijing community, and analyse its influencing factors, so as to provide reference for further intervention. We investigated 254 older people with type 2 diabetes in Xinjiekou community, Beijing, including their general information, chronic complications, foot care knowledge and behaviour.

Multifunctional hyaluronic acid microneedle patch enhances diabetic wound healing in diabetic infections.

Diabetic wounds often exhibit a chronic non-healing state due to the combined effects of multiple factors, including hyperglycemia, impaired angiogenesis, immune dysfunction, bacterial infection, and excessive oxidative stress. Despite the availability of various therapeutic strategies, effectively managing the complex and prolonged healing process of diabetic infected wounds remains challenging. In this study, we combined the natural antidiabetic drug lipoic acid (LA) with the RADA16-YIGSR (RY) peptide obtained through solid-phase synthesis, utilizing reversible hydrogen bonds and coordination bonds for binding.

Hapten prediction, monoclonal antibody preparation, and development of an immunochromatographic assay for the detection of fenamiphos.

Fenamiphos (FENA) is an organophosphorus insecticide, and its residues in fruits, vegetables, and the environment have raised concerns. Therefore, it is very important to develop a simple, rapid, and accurate method for FENA detection. In this study, a novel FENA hapten was designed and predicted based on computer-aided simulation technology, and high-performance anti-FENA monoclonal antibodies were screened using a matrix effect-enhanced screening method, with a half-maximal inhibitory concentration of 1.

Single-Cell and Bulk Transcriptomics Reveal the Immunosenescence Signature for Prognosis and Immunotherapy in Lung Cancer.

Background: Immunosenescence is the aging of the immune system, which is closely related to the development and prognosis of lung cancer. Targeting immunosenescence is considered a promising therapeutic approach.

Methods: We defined an immunosenescence gene set (ISGS) and examined it across 33 TCGA tumor types and 29 GTEx normal tissues.