An integrated single-cell and spatial transcriptomic atlas of thyroid cancer progression identifies prognostic fibroblast subpopulations.

Thyroid cancer progression from curable well-differentiated thyroid carcinoma to highly lethal anaplastic thyroid carcinoma is distinguished by tumor cell de-differentiation and recruitment of a robust stromal infiltrate. Combining an integrated thyroid cancer single-cell sequencing atlas with spatial transcriptomics and bulk RNA-sequencing, we define stromal cell subpopulations and tumor-stromal cross-talk occurring across the histologic and mutational spectrum of thyroid cancer. We identify distinct inflammatory and myofibroblastic cancer-associated fibroblast (iCAF and myCAF) populations and perivascular-like populations.

Exploring Stibanyl Ligand for Accessing Arsinidene and Arsaketene Adducts, and Phosphaketene.

The salt metathesis reaction involving a diamine-based antimony chloride precursor with sodium arsaethynolate in the presence of PMe leads to the formation of stibanyl-functionalized PMe-arsinidene (). Detailed analyses through single-crystal X-ray diffraction and density functional theory of confirm the presence of covalent Sb-As bonds and reveal its polarized nature with a multiple-bond character. In contrast to the formation of complex , substituting PMe with xylyl isocyanide or 1,3-diisopropyl-4,5-dimethyl-imidazolin-2-ylidene () produces an isocyanide-arsinidene adduct () and an -arsaketene complex (), respectively.

Chromosome-Level Assembly and Annotation of the Endangered Red-Wing Fish ().

The red-wing fish (), an endangered species native to Yunnan, is endemic to Chenghai Lake. The natural population of this species has suffered a sharp decline due to the invasion of alien fish species. Fortunately, the artificial domestication and reproduction of have been successful and this species has become an economic species locally.

Post-transcriptional modifications on tRNA fragments confer functional changes to high-density lipoproteins in atherosclerosis.

Epitranscriptomic modifications on RNA play critical roles in stability, processing, and function, partly by influencing interactions with RNA-binding proteins and receptors. The role of post-transcriptional RNA modifications on cell-free non-coding small RNA (sRNA) remains poorly understood in disease contexts. High-density lipoproteins (HDL), which transport sRNAs, can lose their beneficial properties in atherosclerosis cardiovascular disease (ASCVD).