Acquisition and transcriptomic analysis of tissue micro-regions using a capillary-based method.

Profiling the site-specific transcriptomes of microregions of interest (mROIs) contributes to a complete understanding of multicellular organisms. However, the simple and efficient isolation of mROIs for spatially detecting gene expression remains challenging. Here, we develop an efficient capillary-based microdissection system (CMS) for precisely isolating targeted samples from tissue sections.

Ultra-fast [F]florbetapir PET imaging using the uMI Panorama PET/CT system.

Background: There is a need for faster amyloid PET scans to reduce patients' discomfort, minimize movement artifacts, and increase throughput. The recently introduced uMI Panorama PET/CT system featuring enhanced spatial resolution and sub-200ps TOF offers the potential for shorter scan duration without sacrificing image quality or efficacy to detect Aβ deposition. The study aims to establish a faster acquisition protocol for [F]florbetapir PET imaging using digital PET/CT scanner uMI Panorama, while ensuring adequate image quality and amyloid-β (Aβ) detectability comparable to the standard 10-minute scan.

Characteristics of OSA modified starch-based Pickering emulsion and its application to myofibrillar protein gel.

Background: Pickering emulsions prepared with octenyl succinic anhydride-modified starch (OSAS) show significant promise as replacements for animal fat. However, the underlying mechanism of incorporating an OSAS-based Pickering emulsion into a myofibrillar protein (MP) gel and its impact on the gel properties remain poorly understood. In this study, the effects of OSAS at varying concentrations (0-10.

Chuanxiong Rhizoma regulates ferroptosis and the immune microenvironment in ischemic stroke through the JAK-STAT3 pathway.

Ferroptosis is linked to various pathological conditions; however, the specific targets and mechanisms through which traditional Chinese medicine influences ischemic stroke (IS)-induced ferroptosis remain poorly understood. In this study, data from the Gene Expression Omnibus and disease target databases (OMIM, GeneCards, DisGeNet, TTD, and DrugBank) were integrated with ferroptosis-related gene datasets. To identify key molecular targets of Chuanxiong Rhizoma (CX), drug ingredient databases, including PubChem and TCMBank, were employed to map CX-related targets (CX-DEGs-FRG and CX-IS-FRG).

[Source and Health Risk Assessment of Heavy Metals in Groundwater of Datong Basin].

The identification of distribution characteristics, pollution sources, and potential human health risks of heavy metals in groundwater is crucial for the scientific planning and rational development of groundwater resources in arid-semiarid regions. In this study, 46 groundwater samples were collected and analyzed using hydrogeochemical modeling and multivariate statistical analysis methods to reveal the pollution characteristics and speciation distribution of 11 heavy metals （As, B, Pb, Sb, Tl, Mn, Ba, Cd, Co, Cr, and Al） in the Datong Basin. The absolute principal component-linear regression （APCS-MLR） model and health risk assessment model （HRA） were employed to determine the sources and health risk levels of heavy metals in groundwater.