Simultaneous Determination of Underivatized Vitamin B1 and B6 in Whole Blood by Reversed Phase Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry.

Background: Vitamin B1 (thiamine-diphosphate) and B6 (pyridoxal-5'phosphate) are micronutrients. Analysis of these micronutrients is important to diagnose potential deficiency which often occurs in elderly people due to malnutrition, in severe alcoholism and in gastrointestinal compromise due to bypass surgery or disease. Existing High Performance Liquid Chromatography (HPLC) based methods include the need for derivatization and long analysis time.

The analysis of thiamin and its derivatives in whole blood samples under high pH conditions of the mobile phase.

In this study a protocol for the analysis of thiamin and thiamin coenzymes in whole blood was developed. Thiamin and its coenzymes are analyzed by reversed phase liquid chromatography (RPLC), precolumn derivatisation with alkaline potassium ferricyanide and fluorescence detection, all at pH 10. Under these relatively high pH conditions the detectability of the analytes and the robustness of the method were substantially improved.

Experimental model of colon cancer: recurrences after surgery alone or associated with intraperitoneal 5-fluorouracil chemotherapy.

The liver is the most frequent site of metastases in colon cancer. No good animal model has been available to help improve the treatment of liver metastases or their prevention after resection of a primary colon cancer. The aim of this study was to develop a model of colon cancer induced by azoxymethane in the rat and to study the outcome after surgical resection alone or in association with intraperitoneal chemotherapy (5-fluorouracil (5-FU).

The isolated perfused rat spleen. An original method for studying the function of hepatocytes transplanted into the spleen.

The aim of this study was to assess directly the function of isolated hepatocytes 1 year after transplantation into the spleen, using an original model of isolated rat-spleen perfusion. Three specific liver functions, albumin synthesis, indocyanine-green clearance, and antipyrine oxidation, were studied. Five x 10(6) isolated hepatocytes were injected into the spleen of syngenic Wistar-Furth rats.

The healing of colonic anastomoses after early intraperitoneal chemotherapy: an experimental study in rats.

Early postoperative intraperitoneal administration of 5-fluorouracil (5-Fu) is a logical adjuvant treatment of patients with resectable colonic cancers. It is easier and less invasive than the intraportal administration of the drug. However, before applying the procedure to humans it must be demonstrated than it does not disturb the healing of recent colonic anastomoses.

Application of antibodies to intermediate filament proteins in simple and complex tumors of the female genital tract.

Polyclonal antibodies to cytokeratins, vimentin, and desmin and monoclonal antibodies to vimentin and to individual cytokeratin polypeptides, specific for glandular epithelia (RGE 53) or kertinizing stratified squamous epithelia (RKSE 60), have been applied in gynecological tumors with simple or complex composition. In general, tumors with simple composition showed reaction patterns fitting their known epithelial or mesenchymal nature, i.e.

Immunohistochemical identification of Langerhans cells in normal epithelium and in epithelial lesions of the uterine cervix.

Using the double label indirect immunofluorescence technique we have studied vimentin-positive cells present in normal ecto- and endocervical epithelium, subcolumnar reserve cell hyperplasia, and squamous metaplastic and dysplastic epithelium of the uterine cervix. Monoclonal antibodies to Ia- and T6-antigens were applied in the examination of the expression of these membrane markers by such cells. Our studies reveal the presence of a relatively large number of vimentin-positive and T6-positive (Langerhans) cells in normal ectocervical stratified squamous epithelium, a small number in endocervical columnar epithelium, and a larger number in subcolumnar reserve cell hyperplasia and in immature squamous metaplasia.

Cytoskeletal proteins as tissue-specific markers in cytopathology.

Antibodies to intermediate filament proteins react in a tissue-specific manner and can be used to characterize tumor cells present in thin-needle aspirates from solid tumors, from palpable lymph nodes and cells present in samples from peritoneal and pleural effusions. From our studies so far the following conclusions can be drawn: Polyclonal antisera to cytokeratins can identify carcinoma metastases in thin-needle aspirates from palpable lymph nodes and distinguish them from malignant lymphomas and nonmalignant lesions such as chronic lymphadenitis, which show only vimentin-positive cells. Monoclonal antibodies to specific cytokeratin polypeptides are able to distinguish between different types of epithelial tumor metastases, i.

An acardiac acephalic monster.

A case of an acardiac acephalic monster is described, and the literature concerning the incidence, classification and etiology of acardia is reviewed. Acardia is a very rare congenital anomaly occurring in less than 1 in 34600 deliveries. The acardiac monster has been reported only in multiple, monochorionic pregnancies.

A case of acardiac anomaly in the cynomolgus monkey (Macaca fascicularis): a complication of monozygotic monochorial twinning.

A case of acardius acephalus in a Macaca fascicularis is reported. This congenital anomaly occurs only in multiple-birth gestations (most commonly monozygotic twins). Artery-to-artery and vein-to-vein anastomoses appear to be a constant feature.

Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix.

Polyclonal and monoclonal antibodies to cytokeratin polypeptides were used to study the expression of these intermediate filament proteins in normal, squamous metaplastic, and neoplastic epithelium of the uterine cervix, in order to investigate the morphogenesis of early epithelial changes preceding cervical squamous cell carcinoma. A polyclonal keratin antiserum showed a positive reaction in all different epithelial cell types of the uterine cervix. A positive reaction was also found in subcolumnar reserve cell hyperplasia, in squamous metaplastic and dysplastic cells, and in (squamous) carcinoma in situ.

Sialocystadenoma papilliferum of the palate.

The clinical and histological data concerning a case of sial -( ocyst )-adenoma papilliferum of the palate are presented. Ten cases of sialadenoma papilliferum have been reported to date. The lesion is characterized by several distinct clinical and histological features resembling in many aspects the syringocystadenoma papilliferum of the skin.

Leiomyosarcoma of spermatic cord.

A case of leiomyosarcoma of the spermatic cord is presented, bringing the total number of reported cases to 39. A review of diagnostic and therapeutic approaches along with our experiences stress the need for a uniform treatment schedule.

Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview.

Intermediate-sized filament proteins (IFP) are tissue specific in that antibodies to keratin, vimentin, desmin, glial fibrillary acidic protein (GFAP) and the neurofilament proteins can distinguish between cells of epithelial and mesenchymal origin as well as of myogenic and neural origin respectively. Malignant cells retain their tissue-specific IFP, which makes it possible to use these antibodies in tumour diagnosis. Carcinomas are exclusively detected by antibodies to keratin.

Demonstration of keratin in human adenocarcinomas.

The occurrence and localization of intermediate-sized filaments in 85 cases of adenocarcinoma have been examined by the indirect immunofluorescence technique as well as by the immunoperoxidase technique. Frozen sections of human tumor tissue were incubated with antibodies to keratin, vimentin, and desmin. In contrast to earlier studies by Schlegel et al, this study demonstrates the presence of keratin in 64 cases of primary adenocarcinoma, including tumors of stomach, colon and rectum, lung, pancreas, bile ducts, ovary and uterus, female breast, and prostate, and in 21 cases of adenocarcinomatous metastases in lymph nodes, thoracic and abdominal wall, omentum, mesentery, testis, liver, and the pelvis.

Intermediate filaments in malignant melanomas. Identification and use as marker in surgical pathology.

Intermediate-sized filaments have been studied in human malignant melanomas and in normal melanocytes by immunofluorescence microscopy with antibodies directed against keratin, vimentin, desmin, neurofilament protein, and glial filament protein. Both human melanotic and amelanotic tumor cells and tumor metastases as well as normal melanocytes in human skin and in the rat eye contain exclusively intermediate filaments of the vimentin type. No reaction was seen with antibodies to keratin, desmin, neurofilaments, or glial filaments.

Differential diagnosis of human carcinomas, sarcomas and their metastases using antibodies to intermediate-sized filaments.

Intermediate filaments (IF) are tissue-specific in so far that epithelial, mesenchymal, muscle and neural tissue types can be distinguished by the use of specific antibodies to keratin, vimentin, desmin and neurofilaments or glial filaments respectively. We have examined the possibility of using these sera in the differential diagnosis of human malignant tumors. Using antisera to human skin keratin and bovine lens vimentin we could differentiate between carcinomas (keratin +) and sarcomas (vimentin +).