In-vivo immunocompatibility and induced regenerative potential of silk fibroin modified decellularized porcine liver scaffolds in rat subcutaneous and full-thickness abdominal wall defect models.

For reconstructive surgical applications, humoral and cell-mediated immune response to scaffolds is important in determining its structural and functional integration and performance. A decellularized porcine liver matrix(DPL) mechanically augmented with impregnating silk fibroin(SF100DPL) and silk fibroin-gelatin blends(SFG5050DPL and SFG3070DPL) following citric acid crosslinking were evaluated in-vitro and in-vivo (subcutaneous and abdominal wall defect models) in comparison to unmodified DPL. Ensuring the preservation of glycosaminoglycan and the potential to induce cell migration in L929 cell line, the host immunocompatibility of the scaffolds was confirmed by implanting sub-cutaneously in rat.

Combinatorial Decellularization as a Better Approach to Porcine Liver Extracellular Matrix Scaffold Fabrication With Preserved Bioactivity: A Comparative Evaluation.

Soft tissue repair patches of decellularized extracellular matrices (ECM) with inherently preserved structural components and biomacromolecules are desirable in regenerative applications. This study characterizes three detergent-based decellularization methods to fabricate acellular porcine liver matrices to remove antigenic determinants without compromising the structural integrity, glycosaminoglycans (GAG) content, and bound growth factors within the resulting ECM. Three detergents chosen for decellularization were sodium dodecyl sulfate (SDS), SDS with sodium deoxycholate (SDS + SDC-combinatorial method), and Triton X-100 followed by SDS.

A Nontoxic and Biocompatible Method for Augmenting Mechanical Strength of Acellular Matrix by Silk Fibroin Impregnation.

Biological scaffolds are plagued by poor biomechanical properties and untimely degradation. These limitations have yet to be addressed without compromising their biocompatibility. It is desirable to avoid inflammation and have degradation with concomitant host collagen deposition or even site-appropriate regeneration for the successful outcome of an implanted biological scaffold.