Numerical simulation of airflow and particle distributions with floor circular swirl diffuser for underfloor air distribution system in an office environment.

In the present study, an underfloor air distribution (UFAD) system with a circular swirl diffuser was simulated in an office room using a three-dimensional model when thermal sources were present in the room. An Eulerian-Lagrangian model was used to predict the characteristics of air and particle phases. In the Lagrangian particle model, the effects of drag, lift, Brownian, and thermophoresis forces were considered.

Potent inhibitory ligands of the GRB2 SH2 domain from recombinant peptide libraries.

We cloned and expressed the SH2 domain of human GRB2 as glutathione S-transferase and maltose binding protein fusion proteins. We screened three phagemid-based fd pVIII-protein phage display libraries against SH2 domain fusion proteins. Sequence analysis of the peptide extensions yielded a variety of related peptides.

Characterization of the roles of SH2 domain-containing proteins in T-lymphocyte activation by using dominant negative SH2 domains.

Activation of the T-cell antigen receptor initiates a complex signaling cascade leading to changes in cytokine gene transcription. Several proteins containing Src homology 2 (SH2) domains, capable of interacting with phosphotyrosine-containing sequences within other proteins, are involved in the activation of signaling intermediates such as p2l(ras) and phospholipase Cgamma1. In this study, we used dominant negative SH2 domains to determine the importance of SH2 domain-containing proteins for T-cell activation.