Publications by authors named "Puspa Wardhani"
Background: Coronavirus disease (COVID-19) induces inflammation, coagulopathy following platelet and monocyte activation, and fibrinolysis, resulting in elevated D-dimer levels. Activated platelets and monocytes produce microvesicles (MVs). We analyzed the differences in platelet and monocyte MV counts in mild, moderate, and severe COVID-19, as well as their correlation with D-dimer levels.
View Article and Find Full Text PDFDengue virus infection (DVI) remains a significant health challenge, and diagnosis must still be considered. Non-structural protein 1 (NS1) is a potential marker of the dengue virus that can help diagnose DVI. The study aimed to assess the role of NS1 as a predictor of the severity of DVI.
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- The Xpert Xpress SARS-CoV-2 rapid test is FDA-approved for emergency use and offers faster results using the RT-PCR method compared to traditional testing.
- A study conducted in Indonesia found that this rapid test reliably detects COVID-19, showing perfect compatibility with the Abbott M2000 RT-PCR system.
- The results indicate the Xpert Xpress has a sensitivity and specificity of 100%, making it highly effective for diagnosing COVID-19.
Background: SARS-CoV-2 can trigger a dysfunctional immune response in COVID-19 patients and lead to immunosuppression. HLA-DR molecule expressed on the surface of monocytes, known as mHLA-DR, has been widely used as a reliable marker of immunosuppression. Downregulation of mHLA-DR reflects an immunosuppressed state.
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- * Researchers used next-generation sequencing to analyze 13 complete genomes of DENV-4 from the outbreak, focusing on its evolutionary patterns and genetic changes.
- * Findings revealed that selection pressure and genetic mutations, along with possible recombination events in the virus, may explain DENV-4's prevalence in East Java during the outbreak.
Article Synopsis
- The study aimed to identify factors that influence how stroke patients adapt and their overall quality of life six months after discharge.
- The research involved 80 patients and their caregivers, using medical records and path analysis to examine the relationships between factors affecting adaptation responses.
- Key findings revealed that self-efficacy significantly impacted both psychosocial and physiological adaptations, contributing heavily to the quality of life for stroke survivors.
Background And Aim: Some individuals in Indonesia consume intact goat gallbladder to prevent and treat malaria. The acute and subacute toxicity tests of goat bile (GB) have shown mild diarrhea in mice. Therefore, this study aimed to evaluate the suppressive effect of GB on parasitemia, splenomegaly, hepatomegaly, and blood biochemistry to assess liver and kidney function in BALB/c mice infected with ANKA.
View Article and Find Full Text PDFBackground: The diagnostic test for malaria is mostly based on Rapid Diagnostic Test (RDT) and detection by microscopy. Polymerase Chain Reaction (PCR) is also a sensitive detection method that can be considered as a diagnostic tool. The outcome of malaria microscopy detection depends on the examiner's ability and experience.
View Article and Find Full Text PDFOutbreaks of dengue virus (DENV) in Indonesia have been mainly caused by the DENV serotype-1; -2; or -3. The DENV-4 was the least-reported serotype in Indonesia during the last five decades. We recently conducted a molecular epidemiology study of dengue in the Jember regency, East Java province, Indonesia.
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- The study aimed to investigate the toxicity of goat bile in BALB/c mice, as some people in Indonesia consume it for health benefits.
- An acute toxicity test showed no mouse deaths, with only mild diarrhea noted; however, effects on blood parameters were observed at certain concentrations of goat bile.
- The conclusion suggests that while goat bile caused mild toxicity, its consumption should be limited to avoid potential harmful effects.
Article Synopsis
- * Researchers reported the presence of all four dengue virus serotypes in Surabaya, Indonesia, with a notable predominance of DENV-1, which was further classified into two distinct genotypes.
- * Full-length genomic sequencing revealed recent introduction of DENV-1 Genotype I and strong purifying selection on most codons, with unique sites under positive selection, enhancing understanding of dengue dynamics in the region.
Article Synopsis
- Aedes aegypti and Aedes albopictus are key mosquito species that transmit dengue, a significant global viral disease, with Aedes aegypti being the primary vector in Surabaya, Indonesia.
- A large-scale study collected over 16,600 mosquitoes from 2008 to 2015, revealing that DENV-1 was the most common serotype found, along with fluctuations in DENV types over the years.
- The research suggests that monitoring these infected mosquitoes can help predict potential future dengue outbreaks, as the circulating dengue serotypes tended to align with those seen in human cases.
Dengue disease is still a major health problem in Indonesia. Surabaya, the second largest city in the country, is endemic for dengue. We report here on dengue disease in Surabaya, investigating the clinical manifestations, the distribution of dengue virus (DENV) serotypes, and the relationships between clinical manifestations and the genetic characteristics of DENV.
View Article and Find Full Text PDFDengue is a febrile disease caused by infection of dengue virus (DENV). Early diagnosis of dengue infection is important for better management of the disease. The DENV Non-Structural Protein 1 (NS1) antigen has been routinely used for the early dengue detection.
View Article and Find Full Text PDFDiagnostic tests based on detection of dengue virus (DENV) genome are available with varying sensitivities and specificities. The Simplexa Dengue assay (Focus Diagnostics) is a newly developed real-time RT-PCR method designed to detect and serotype DENV simultaneously. To assess the performance of the Simplexa Dengue assay, we performed comparison with conventional RT-PCR and SYBR Green real-time RT-PCR on patients sera isolated from eight cities across Indonesia, a dengue endemic country.
View Article and Find Full Text PDFBackground: Early diagnosis of dengue infection is crucial for better management of the disease. Diagnostic tests based on the detection of dengue virus (DENV) Non Structural Protein 1 (NS1) antigen are commercially available with different sensitivities and specificities observed in various settings. Dengue is endemic in Indonesia and clinicians are increasingly using the NS1 detection for dengue confirmation.
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