The present study is carried out to ascertain the effect of different physical factors (sunlight, temperature, pH) and storage conditions on the antimicrobial efficacy of leaf extracts and bio-formulation against the . In addition, the phytotoxic potential of 100% alcoholic crude extract as well as the acetone fraction of young leaves of was also checked on seed germination of chilli (). Results showed that there was no adverse effect of wet heat (50-100 °C) and dry heat (40-90 °C) on extract and bio-formulation efficacy.
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