[Rapid diagnosis of ophthalmic herpes].

The developed method for rapid diagnosis of ophthalmic herpes is based on quantitative estimation of the results of immunofluorescent studies of the cells in scrapings off the eye conjunctiva. A total of 213 patients were examined with the use of this method; herpes simplex virus antigen was detected in 56.5%, but only in 23.

[Preparative isolation of purified antigens of the herpes simplex virus type I and a study of their immunogenic properties].

The HSV-I-containing material prepared in chick embryo fibroblast cultures was concentrated on nuclear filters followed by chromatography on a column with large-pore silica. The MESK detergent was used for isolation of glycoproteins. The glycoprotein preparation was highly immunogenic in experimental animals.

[Effect of ribavirin on the reproduction of influenza B virus].

The influence of ribavirin on influenza B virus reproduction was studied at different experimental levels. Ribavirin was found to reduce significantly the production of hemagglutinin and accumulation of infectious influenza B/Lee/40 virus in MDCK cell culture. Synthesis of virus-specific polypeptides of influenza B/Hong Kong/5/72 virus was markedly reduced in its presence.

[Isolation of natural cold variants from influenza virus strains similar to A/Bangkok/1/79 (H3N2)].

Two natural cold variants were isolated from influenza virus strains similar to A/Bangkok/1/79 (H3N2) and their antigenic and some biological properties were studied in comparison with the original strains. No correlation between the temperature sensitivity of the strains and other properties was found.

[Effectiveness of a combination of remantadine and ribamide administered by aerosol in experimental influenzal infection in white mice].

The effectiveness of remantadine, ribamide, and combination there of administered in aerosol on experimental influenza A/Aichi/2/68 virus infection in white mice was studied. Each of the drugs used alone was found to produce significant reduction in the mortality rate. Use of the drugs in combination both for therapeutic-prophylactic and for therapeutic purposes is of the additive nature, reducing the mortality of the animals by 50% as compared with the control group.

[Combined action of remantadine (amantadine) and ribavirin on experimental influenza].

The inhibiting effect of remantadine (amantadine) and ribavirin combination on experimental influenza infection was studied in comparison of the effect of each of these drugs alone. The combination of the two chemopreparations was found to inhibit synthesis of influenza A virus proteins in cell culture more effectively, to ensure maximum survival rate of white mice treated with these preparations by oral, aerosol and intraperitoneal routes of administration.

[Effect of remantadine, ribavirin and their combinations on the protein synthesis of influenza virus A in a cell culture].

Simultaneous use of ribavirin (I-beta-D-ribofuranosyl-1,2,4-triazol-3-carboxamide and rimantadine (alpha-methyl-I-adamantane methylamine HCL) was shown to effect most distinctly on protein synthesis in influenza virus A in the system studied as compared with the separate action of each of the drugs. Ribavirine inhibited the protein synthesis in rimantadine-resistant strain of influenza virus A.

[Comparative study of the polypeptide composition of influenza A viruses sensitive and resistant to remantadine].

The polypeptide composition of influenza A FPV (Hav1N1) and A/Texas/1/77 (H3N2) viruses which had acquired resistance to remantadine after serial passages in cell cultures in the presence of the drug was studied. It was found that in parallel with the acquired resistance to remantadine the molecular weight of the heavy chain of hemagglutinin changed only in the course of cell culture passages. The influenza A/Texas/1/77 virus passaged in chick embryos in the presence of remantadine exhibited no differences in the electrophoretic mobility of hemagglutinin between the sensitive and resistant variants.

[Prevention of the formation of a mutant influenza A virus resistant to rimantadine (alpha-methyl-1-adamantanemethylamine hydrochloride) by using ribavirin (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide)].

The paper presents the results of the studies on the possibility of the development of influenza A virus resistance to effective anti-influenza drugs remantadine and ribavirine in serial passages in cell culture. Influenza virus forms resistant to remantadine were shown to develop quite rapidly. No formation of resistance to ribavirine was observed.

[Effect of 1-D-ribofuranosyl-1,2,4-triazol-3-carboxamide on the synthesis of cellular macromolecules in cell culture].

Ribovirine (I-D-ribofuranosyl-1,2,4-triazol-3-carboxamide) possesses the distinct antiviral action against many strains of RNA- and DNA-containing viruses. Data on the effect of ribovirine on the synthesis of cellular macromolecules are presented. After treatment with ribovirine the synthesis of cellular RNA was inhibited by 80-85%.

[Study of the sensitivity of different strians of the influenza A2 virus of rimantadine].

The sensitivity of different influenza A2 (H3N2) virus strains to rimantadine in ovo was studied. The reference strains of influenza virus A/Hong Kong/1/68, A/England/42/72, A/Scotland/840/74 as well as new epidemic strains isolated in the USSR and Mongolia in 1974-1975 antigenically related to influenza A/Port Chalmers/1/73 virus were found to be sensitive to rimantadine.

[Inhibitory effect of alpha-methyl-1-adamantane methylamine hydrochloride (rimantadine) on RNA-dependent RNA polymerase induction in culture of cells, infected with influenza virus].

An anti-influenza preparation, rimantadine (alpha-methyl-1-adamantane methylamine hydrochloride) at concentrations of 10--25 mkg/ml depresses the RNA-dependent RNA polymerase induction in a culture of cells infected with influenza virus (fowl plague virus). The inhibitory effect is also observed 2 hours following cell infection. In vitro studies have demonstrated that rimantadine has no effect on the activity of virus-induced RNA-dependent RNA polymerase, as well as on that of RNA-dependent RNA polymerase associated with virus particles.