[The action of adrenergic substances on the smooth muscle of the rat trachea].

Adrenalin and noradrenaline first induced an increase in the trachea smooth muscle response with its subsequent decline in rats. Phentolamine decreases the muscle responses in its thoracic part and practically does not affect it in the cervical segments. The adrenergic neural fibres seem to terminate at the smooth muscle cells and the neurons of intramural ganglia.

[The neuronal impulse activity of the subepicardial plexus in the isolated heart of the cat].

The unit activity recorded in the right atrium plexus of the Langendorff preparation was spontaneously irregular in 20 per cent of the neurons. Hexamethonium abolishes it. The spontaneous activity and vagus-induced discharges were inhibited by adrenaline or by stimulation of the stellate ganglion in nearly 30 per cent of the neurons.

[The age-related characteristics of changes in osmotic and ionic homeostasis in spontaneously hypertensive rats].

The 3-month old SHR differ from the WKY rats in that the former have an increased osmolality, concentration of albumins, activity (but not the concentration) of sodium and a reduced activity of potassium. The shifts in the albumins concentration occur, mainly, on account of the amino acid phenylalanine. The gradient increase of the sodium and potassium concentration between the plasma and muscular tissue in followed by a slight hyperpolarisation of the muscle fibers, a drop in the lability and a increase in pO2.

[The functional properties of the intramural bladder neural plexus].

In the cat isolated bladder, decentralization of the bladder plexus first decreased spontaneous unit activity. Later, after degeneration of preganglionic nervous fibres, intensified functioning of own reflex arches of the plexus maintained the coordination of activity in different areas of the bladder. Due to an increased excitability and chemosensitivity, the neurons are to a larger extent recruited in the activity of the reflex arches.

[Homosynaptic depression--a model of the habituation phenomenon].

A high functional stability of the cat spinal cord motoneurons was shown in conditions of long-term orto- and antidromic activation with frequency corresponding to their natural rhythmicity. The depression of reflex discharges was shown to be related not to any postsynaptic depression or presynaptic inhibition but to a drop of probability of the transmitter release from the primary afferents' terminals. Analysis of signs of the habituation phenomenon revealed their similarity to the homosynaptic depression phenomenon and, consequently, their submittance to the same principles of experimental analysis and consideration.

[Effect of neuropeptides on the motor output of the buccal ganglia of freshwater mollusks].

Hypertensin, gastrin-releasing peptide, neuromedin and substance P activated the buccal ganglia in Planorbis corneus and Lymnaea stagnalis, whereas secretin, somatostatin, pancreosimin and octapeptide of cholecystokinin suppressed them. No effect of neurotensin and alitesine were found. The peptides seem to rearrange functioning of buccal nervous network.

[Processes facilitating conduction in the vesical plexus of the cat].

In the cat isolated bladder plexus, cholinergic transmission from parasympathetic "entry" was shown to be less obvious at low-frequency stimulation and much more effective (up to 300-1800%) at a high-frequency one (1-20/sec). During posttetanic potentiation (up to 5 min) it is possible to reach the 100% saturation of ganglion motor pool. The phenomena of facilitation were shown to be conducted through presynaptic mechanisms.

[Adrenergic mechanisms of the cystic plexus in the cat].

Hypogastric and pelvic nerves were stimulated in experiments carried out on isolated vesical plexuses of the cat as well as intra-or extracellular recording of the activity of vesical ganglion neurons was conducted. Characteristics of evoked responses, ganglionic lability, rhythmic and posttetanic potentiation were studied. Homosynaptic presynaptic origin of posttetanic potentiation was noted.

[Reactive changes in autonomic synapses during frequency electric stimulation].

Reactive modifications of synapses of isolated intratrunkal vegetative neurones induced by high frequency orthodromal electric stimulation have been examined by vital phase-contrast microscopy, electron microscopy, vital staining with methylene blue in conjunction with concurrent electrophysiological control Flocculent aggregation of the synaptoplasma and vesicles has been discovered, which is associated with higher adhesiveness of modified proteins. There have been also discovered matrix translucence and rounding of the living synaptic buds, which is accounted for by segregation of the hydration fraction of the neuroplasma. The decrease in the number of light synaptic vesicles is accompanied by the appearance of osmiophilic, myelin-like bodies, dense-core vesicles and glycogen-like granules.

[Biochemical diagnosis of glomerular and tubulo-interstitial impairments of kidney].

Using polyacrylamide gel disc electrophoresis in presence of sodium dodecylsulfate (SDS) urinary proteins were studied in apparently healthy persons, in patients with chronic glomerulonephritis, chronic interstitial nephritis as well as in patients with myeloma, urine of which contained monoclonic light chains of immunoglobulins. SDS polyacrylamide gel disc electrophoresis is a suitable procedure for differential diagnosis of glomerular and tubulo-interstitial impairments of kidney. In some cases the procedure enables to ascertain not only the type but also severity of the kidney impairment as well as to study dynamics of the disease without a recourse to such invasive methods as kidney biopsy.

[Electrophysiologic analysis of mediator stores and release in smooth muscle synapses of the nictitating membrane in cats].

At the adrenergic junction of the sympathetic nerve with the medial smooth muscle of the cat nictitating membrane, blocking of the reuptake of noradrenaline with phenoxibenzamine suppressed the amplitude of rhythmic excitatory synaptic potentials. Stimulation of postganglionic sympathetic nerve (10/sec) made the time constant of the first component of the potential 60 sec whereas that of the second component--600 sec. The depression of amplitude seems to be connected with possible decrease in the noradrenaline release and partial exhaustion of its storage.

[Monosynaptic reflexes of the cat spinal cord during the development of insulin hypoglycemia].

Insulin-induced hypoglycemia caused depression of rhythmic monosynaptic EPSP motoneurons of the lumbar cord in acute experiments on narcotized and spinal cats. It was demonstrated that growing depression of monosynaptic transmission was associated with the exhaustion of mediator operative fraction and not with any pre- or postsynaptic delay or inhibition over a period of initial hypoglycemia when the sugar content in the blood fell to the level of 50--60 mg%. The function disturbance of postsynaptic formations of monosynaptic reflex arc of spinal cord occured in more advanced hypoglycemia.