Single-center outcomes of artificial intelligence in management of pulmonary embolism and pulmonary embolism response team activation.

Multidisciplinary pulmonary embolism response teams (PERTs) have shown that timely triage expedites treatment. The use of artificial intelligence (AI) may help improve pulmonary embolism (PE) management with early CT pulmonary angiogram (CTPA) screening and accelerate PERT coordination. This study aimed to test the clinical validity of an FDA-approved PE AI algorithm.

Mammalian phagophores with finger-like shapes emerge from recycling endosomes.

Autophagosomes are double-membraned vesicles that engulf cytoplasmic contents, which are ultimately degraded after autophagosome-lysosome fusion. The prevailing view, largely inferred from EM-based studies, was that mammalian autophagosomes evolved from disc-shaped precursors that invaginated and then were closed at the single opening. Many site(s) of origin of these precursors have been proposed.

Mammalian autophagosomes form from finger-like phagophores.

The sequence of morphological intermediates that leads to mammalian autophagosome formation and closure is a crucial yet poorly understood issue. Previous studies have shown that yeast autophagosomes evolve from cup-shaped phagophores with only one closure point, and mammalian studies have inferred that mammalian phagophores also have single openings. Our superresolution microscopy studies in different human cell lines in conditions of basal and nutrient-deprivation-induced autophagy identified autophagosome precursors with multifocal origins that evolved into unexpected finger-like phagophores with multiple openings before becoming more spherical structures.

Association Between Depression and Anxiety Status With Uptake of Colorectal Cancer Screening Among US Adults: A Population-Level Study.

Background: Individuals with different mental disorders tend to experience higher rates of colorectal cancer (CRC)-related mortality compared to the general population. Discrepancies in CRC screening behaviors have been suggested as a potential contributing factor to this difference in mortality. However, existing evidence on this topic has been inconclusive and conflicting.

Effects of osteoblast-derived extracellular vesicles on aggressiveness, redox status and mitochondrial bioenergetics of MNNG/HOS osteosarcoma cells.

Osteosarcoma is the most common primary bone malignancy. The crosstalk between osteosarcoma and the surrounding tumour microenvironment (TME) drives key events that lead to metastasization, one of the main obstacles for definitive cure of most malignancies. Extracellular vesicles (EVs), lipid bilayer nanoparticles used by cells for intercellular communication, are emerging as critical biological mediators that permit the interplay between neoplasms and the tumour microenvironment, modulating re-wiring of energy metabolism and redox homeostatic processes.

Forecasting Time Series in Healthcare With Gaussian Processes and Dynamic Time Warping Based Subset Selection.

Modelling real-world time series can be challenging in the absence of sufficient data. Limited data in healthcare, can arise for several reasons, namely when the number of subjects is insufficient or the observed time series is irregularly sampled at a very low sampling frequency. This is especially true when attempting to develop personalised models, as there are typically few data points available for training from an individual subject.

Optical absorption investigations for efficient crystal violet dye removal from wastewater via carbon nanotubes:Montmorillonite-based nanocomposite.

The current study reports a facile method to fabricate functionalized multi-walled carbon nanotubes and montmorillonite clay mineral-based nanocomposite matrix and its detailed characterization using spectroscopic and morphological techniques. The nanocomposites have been studied for their potential applications in the treatment of contaminated water using batch adsorption studies. The investigations conducted using optical absorption spectroscopic measurements for the adsorption process indicate that the nanocomposite matrix can effectively remove almost 98% of the dye from aqueous solution.

The different autophagy degradation pathways and neurodegeneration.

The term autophagy encompasses different pathways that route cytoplasmic material to lysosomes for degradation and includes macroautophagy, chaperone-mediated autophagy, and microautophagy. Since these pathways are crucial for degradation of aggregate-prone proteins and dysfunctional organelles such as mitochondria, they help to maintain cellular homeostasis. As post-mitotic neurons cannot dilute unwanted protein and organelle accumulation by cell division, the nervous system is particularly dependent on autophagic pathways.

Unexplained Hypoxemia in a Patient With Transient Ischemic Attack.

An 84-year-old woman with a medical history of hypertension, hypothyroidism, and transient ischemic attacks presented with right-sided upper and lower extremity weakness for 1 day. She was a lifetime nonsmoker with no known heart disease or chronic lung disease. She had no occupational exposure or pertinent family history.

Gestational weight gain prediction using privacy preserving federated learning.

Gestational weight gain prediction in expecting women is associated with multiple risks. Manageable interventions can be devised if the weight gain can be predicted as early as possible. However, training the model to predict such weight gain requires access to centrally stored privacy sensitive weight data.

Feature selection for unbiased imputation of missing values: A case study in healthcare.

Datasets in healthcare are plagued with incomplete information. Imputation is a common method to deal with missing data where the basic idea is to substitute some reasonable guess for each missing value and then continue with the analysis as if there were no missing data. However unbiased predictions based on imputed datasets can only be guaranteed when the missing mechanism is completely independent of the observed or missing data.

Marijuana induced spontaneous pneumomediastinum.

Spontaneous pneumomediastinum (SPM), a rare occurrence, is defined by the presence of extraluminal gas in the mediastinum in the absence of trauma or underlying parenchymal disease. It is usually benign and has been associated with the inhalation of marijuana, cocaine, and amphetamines. The breathing maneuvers along with cyclical vomiting have been postulated as the underlying etiology.

Dyspnoea with progressive "idiopathic" pulmonary fibrosis.

Telomeres are repetitive nucleotide sequences that prevent chromosomal shortening in cell replication. Short telomeres have been implicated in the pathogenesis of interstitial lung disease. Patients with short telomere related pulmonary fibrosis can have computed tomography (CT) findings inconsistent with pro-typical usual interstitial pneumonia/idiopathic pulmonary fibrosis (UIP/IPF) pattern.

Clomiphene- induced pulmonary embolism.

Pulmonary embolism and deep vein thrombosis are a major cause of hospitalization and cause high mortality. Several risk factors, such as smoking, immobilization, cancer, trauma, OCPs, pregnancy, and surgery, have already been identified. The diagnosis of PE can sometimes rely on high clinical suspicion.

Atypical Pyoderma gangrenosum: a case of delayed recognition.

Pyoderma gangrenosum (PG) is an inflammatory skin condition that is often misdiagnosed as a necrotizing infection. This diagnosis must be considered in any patient with underlying systemic disease who presents with large ulcerating lesions that are unresponsive to antibiotics. Early diagnosis and a multidisciplinary approach to treatment are crucial to achieving improvement in quality of life and minimizing cosmetic morbidity.

Remdesivir in a pregnant patient with COVID-19 pneumonia.

Remdesivir is an antiviral drug currently being studied as a potential treatment of pneumonia caused by infection with SARS-CoV-2. The Adaptive Covid-19 Treatment Trial (ACTT-1) by NIH and the SIMPLE study by Gilead Sciences are two major trials that showed promising results of Remdesivir in the non-pregnant population. We are presenting the case of a pregnant patient who was diagnosed with COVID-19 pneumonia and successfully treated with Remdesivir.

The many manifestations of a single disease: neuroborreliosis.

Lyme disease is a tick-borne illness that occurs in stages, multiple organs and tissue with highly variable clinical presentation. Most commonly, it presents with seventh cranial nerve palsy, often mimicking stroke and atypical rash (erythema migrans). Atypical presentations include abdominal pain, ileus/pseudo-obstruction and constipation thought to be due to autonomic dysfunction.

Use of a Zwitterionic Surfactant to Improve the Biofunctional Properties of Wool Dyed with an Onion ( L.) Skin Extract.

To improve the loadability and antioxidant properties of wool impregnated with onion skin extract, the introduction of SB3-14 surfactant in the dyeing process was evaluated. A preliminary investigation on the surfactant-quercetin interaction indicated that the optimal conditions for dye solubility, stability, and surfactant affinity require double-distilled water (pH = 5.5) as a medium and SB3-14 in a concentration above the c.

Erratum: Author Correction: Mendelian neurodegenerative disease genes involved in autophagy.

[This corrects the article DOI: 10.1038/s41421-020-0158-y.].

A location, location, location mutation impairs DNM2-mediated release of nascent autophagosomes from recycling endosomes.

Elucidation of the membranes contributing to autophagosomes has been a critical question in the field, and an area of active research. Recently, we showed that key events in autophagosome formation, from PtdIns3P formation/WIPI2 recruitment to LC3-GABARAP membrane conjugation, occur on the RAB11A-positive compartment (recycling endosomes). This observation raised the question of how the LC3-positive autophagosome precursors detach from the recycling endosome.

Mendelian neurodegenerative disease genes involved in autophagy.

The lysosomal degradation pathway of macroautophagy (herein referred to as autophagy) plays a crucial role in cellular physiology by regulating the removal of unwanted cargoes such as protein aggregates and damaged organelles. Over the last five decades, significant progress has been made in understanding the molecular mechanisms that regulate autophagy and its roles in human physiology and diseases. These advances, together with discoveries in human genetics linking autophagy-related gene mutations to specific diseases, provide a better understanding of the mechanisms by which autophagy-dependent pathways can be potentially targeted for treating human diseases.

A DNM2 Centronuclear Myopathy Mutation Reveals a Link between Recycling Endosome Scission and Autophagy.

Autophagy involves engulfment of cytoplasmic contents by double-membraned autophagosomes, which ultimately fuse with lysosomes to enable degradation of their substrates. We recently proposed that the tubular-vesicular recycling endosome membranes were a core platform on which the critical early events of autophagosome formation occurred, including LC3-membrane conjugation to autophagic precursors. Here, we report that the release of autophagosome precursors from recycling endosomes is mediated by DNM2-dependent scission of these tubules.

PREgDICT : Early prediction of gestational weight gain for pregnancy care.

Excessive or inadequate Gestational Weight Gain (GWG) is considered to not only put the mothers, but also the infants at increased risks with a number of adverse outcomes. In this paper, we use self-reported weight measurements from the early days of pregnancy to predict and classify the end-of-pregnancy weight gain into an underweight, normal or obese category in accordance with the Institute of Medicine recommended guidelines. Self-reported weight measurements suffer from issues such as lack of enough data and non-uniformity.