Publications by authors named "Pulkit S Bose"
Article Synopsis
- Rubella virus (RV), known as German measles, can cause severe congenital defects if a mother is infected during early pregnancy, but it can be effectively prevented with live attenuated vaccines.
- The potency of these vaccines must be measured accurately, with traditional methods like the TCID(50) assay relying on subjective visual inspection of cell damage, which is time-consuming.
- A new TCID(50) readout method using fluorescent markers to detect RV-induced apoptosis has been developed, along with a mathematical model to enhance measurement accuracy, showing strong agreement with traditional methods and enabling more efficient vaccine potency evaluation.
Influenza A virus interacts with specific types of sialic acid during attachment and entry into susceptible cells. The precise amino acids in the hemagglutinin protein that control sialic acid binding specificity and affinity vary among antigenic subtypes. For H3 subtypes, amino acids 226 and 228 are critical for differentiating between alpha2,3- and alpha2,6-linked forms of sialic acid (SA).
View Article and Find Full Text PDFUsing cryo-EM to measure the dipole potential of a lipid membrane.
Proc Natl Acad Sci U S A
December 2006
The dipole potential of a lipid bilayer membrane accounts for its much larger permeability to anions than cations and affects the conformation and function of membrane proteins. The absolute value of the dipole potential has been very difficult to measure, although its value has been estimated to range from 200 to 1,000 mV from ion translocation rates, the surface potential of lipid monolayers, and molecular dynamics calculations. Here, a point charge probe method was used to investigate the dipole potentials of both ester and ether lipid membranes.
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