In vitro model for evaluating the effect of child formula toothpastes on artificial caries in primary dentition enamel.

Purpose: To determine if the in vitro 10-day pH-cycling model used for permanent teeth could be utilized to evaluate de/remineralization effects, on the enamel of primary teeth, of child formula toothpastes.

Methods: Sound extracted primary anterior teeth were coated with nail varnish, leaving a 1 mm-wide window prior to being placed in a demineralizing solution to produce artificial carious lesions in enamel. The teeth were subsequently cut longitudinally into 100 microm thick sections.