sp. nov., and sp. nov., two new members of the genus isolated from plateau pika ().

Four anaerobic, Gram-stain-positive, non-motile, non-sporulating rod-shaped bacterial strains (R7, R21, R22 and R25) were isolated from the intestinal contents of plateau pika () collected from the Qinghai-Tibet Plateau, PR China. The four isolates grew at between 25 and 42 °C (optimally at 35-37 °C), and with 0.3-3.

sp. nov., sp. nov. and sp. nov., isolated from soil of the Qinghai-Tibet Plateau.

Six novel bacterial strains, designated N016, N017, N022, N028, N056, and N064, were isolated from soil sampled on the Qinghai-Tibet Plateau. Cells were aerobic, orange or yellow, globular or rod-shaped, non-motile, non-spore-forming, Gram-stain-positive, catalase-positive and oxidase-negative. All the isolates were salt-tolerant and could grow in the range of 4-42 °C.

Pannonibacter tanglangensis sp. nov., a New Species Isolated from Pond Sediment.

Two bacterial strains (XCT-34 and XCT-53) isolated from sediment samples of an artificial freshwater reservoir were analyzed using a polyphasic approach. The two isolates are aerobic, Gram-stain-negative, oxidase-negative, catalase-positive, motile with polar flagella, rod-shaped, and approximately 1.4-3.

alleviates hyperglycemia and regulates gut microbiota and metabolic profiles in mice.

Unlabelled: is the dominant species of the genus in the gut, which is genomically heterogeneous and difficult to isolate; hence, scarce research was carried out for this species. This study aimed to investigate the effect of on hyperglycemia. Thirty-nine strains were isolated from healthy individuals, and three strains (HF2123, HF1478, and HF2130) that had the highest glucose consumption were selected to evaluate the effects of supplementation on hyperglycemia.

Species-level Microbiota of Biting Midges and Ticks from Poyang Lake.

Objective: The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area, namely, Qunlu Practice Base, Peach Blossom Garden, and Huangtong Animal Husbandry, and whether vectors carry any bacterial pathogens that may cause diseases to humans, to provide scientific basis for prospective pathogen discovery and disease prevention and control.

Methods: Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit (OPU) analysis, we characterized the species-level microbial community structure of two important vector species, biting midges and ticks, including 33 arthropod samples comprising 3,885 individuals, collected around Poyang Lake.

Results: A total of 662 OPUs were classified in biting midges, including 195 known species and 373 potentially new species, and 618 OPUs were classified in ticks, including 217 known species and 326 potentially new species.

Unveiling the Mutations and Conservation of InlA in .

() is a pathogen that is transmitted through contaminated food and causes the illness known as listeriosis. The virulence factor InlA plays a crucial role in the invasion of into the human intestinal epithelium. In addition, InlA enhances the pathogenicity of host strains, and different strains of contain varying variations of InlA.

Taxonomic descriptions of sp. nov., sp. nov. and sp. nov.

Six Gram-stain-positive, facultative anaerobic, nonmotile and rod-shaped strains, designated zg-Y50, zg-Y1362, zg-Y1379, zg-Y869, zg-629 and zg-Y636, were isolated from the intestinal contents of in Qinghai Province, PR China. Strains zg-Y50, zg-Y1379 and zg-629 exhibited the highest 16S rRNA gene sequence similarities of 99.2, 98.

, gen. nov., sp. nov., a novel bacterial species of the family that could produce flexirubin type pigments.

Two Gram-stain-negative, non-spore-forming, rod-shaped, and obligately aerobic bacteria, designated strains CX-624 and cx-311, were isolated from soil samples in Qinghai Province, China. The two strains grew best at 28 °C on the plate with Tryptone soya agar (TSA). Cells formed circular, convex, translucent, smooth, and orange colonies with approximately 1.

sp. nov. and sp. nov., isolated from the respiratory tracts of marmots.

Four Gram-stain-negative, oxidase-positive, non-motile, cocci-shaped bacteria strains (ZJ106, ZJ104, ZJ785 and ZJ930) were isolated from marmot respiratory tracts. Phylogenetic analyses based on 16S rRNA genes, 53 ribosomal protein sequences and 441 core genes supported that all four strains belonged to the genus with close relatives 10022 and ATCC 51483. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were below the species-level thresholds (95-96 % for ANI, and 70 % for dDDH).

Species-level microbiota of ticks and fleas from in the Qinghai-Tibet Plateau.

Introduction: Ticks and fleas, as blood-sucking arthropods, carry and transmit various zoonotic diseases. In the natural plague foci of China, monitoring of has been continuously conducted in and other host animals, whereas other pathogens carried by vectors are rarely concerned in the Qinghai-Tibet Plateau.

Methods: In this study, we investigated the microbiota of ticks and fleas sampling from in the Plateau, China by metataxonomics combined with metagenomic methods.

Identification of sp. nov. and sp. nov., two new members of the genus .

A polyphasic taxonomic characterization of two novel strain pairs (designated zg-579/zg-578 and zg-536/zg-ZUI104) isolated from the faeces of was conducted based on phylogenetic analysis of the nearly full-length 16S rRNA gene and genome, digital DNA-DNA hybridization, ortho-average nucleotide identity (Ortho-ANI), and phenotypic and chemotaxonomic traits. Comparative analysis of the nearly full-length 16S rRNA gene sequences showed that strain zg-579 was most closely related to FR1436 (97.57 %) and SC8A-24 (97.

sp. nov., sp. nov. and sp. nov., three cellulolytic bacteria isolated from intestinal contents of .

Six facultative anaerobic, Gram-stain-positive, oxidase-negative, rod-shaped bacteria (strains zg-B89, zg-B12, zg-Y338, zg-Y138, zg-Y908 and zg-Y766), were isolated from the intestinal contents of in Qinghai Province, PR China. The 16S rRNA gene sequence analysis showed that zg-B89 showed highest similarity to NBRC 101100 (99.5 %), zg-Y338 to DSM 20118 (98.

Whole-genome sequencing of species isolated from the healthy human eye and description of sp. nov.

Currently, the genus comprises 76 recognized species. Members of are mostly isolated from environmental, animal, and plant sources. This report describes and proposes a novel species of isolated from clinical specimens of the human ocular surface.

Species-Level Taxonomic Characterization of Uncultured Core Gut Microbiota of Plateau Pika.

Rarely has the vast diversity of bacteria on Earth been profiled, particularly on inaccessible plateaus. These uncultured microbes, which are also known as "microbial dark matter," may play crucial roles in maintaining the ecosystem and are linked to human health, regarding pathogenicity and prebioticity. The plateau pika () is a small burrowing steppe lagomorph that is endemic to the Qinghai-Tibetan Plateau and is a keystone species in the maintenance of ecological balance.

Comparative Evaluation of Standard RT-PCR Assays and Commercial Real-Time RT-PCR Kits for Detection of Lassa Virus.

Lassa virus (LASV) is a causative agent of hemorrhagic fever epidemic in West Africa. In recent years, it has been transmitted several times to North America, Europe, and Asia. Standard reverse transcription (RT)-PCR and real-time RT-PCR are extensively used for early detection of LASV.

Description of Ornithinimicrobium cryptoxanthini sp. nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.

Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270) were isolated from the near-surface sediments of river in Qinghai Province, P.

Morphological and genomic characteristics of two novel actinomycetes, sp. nov. and sp. nov. isolated from bat faeces ( and ).

Four Gram-staining-positive, aerobic, non-motile, circle-shaped bacteria were isolated from the faeces of bats ( and ) collected from Guangxi autonomous region (E106°49'20″, N22°20'54″) and Yunnan province (E102°04'39″, N25°09'10″) of South China. Strains HY006 and HY008 shared highly 16S rRNA gene sequence similarity to those of W204 (99.3%) and CPCC 203535 (97.

sp. nov. isolated from the sputum of patients with pulmonary infection.

Two Gram-stain-positive, aerobic and rod-shaped actinomycetes (strains CY18 and CY8) were isolated from the sputum of two patients with pulmonary infections, and their taxonomic status was investigated. The 16S rRNA gene sequences and the results of phylogenetic analyses indicated that CY18 and CY8 were identical (100 %) and were most closely related to CGMCC 4.1521 (99.

The Threat of Potentially Pathogenic Bacteria in the Feces of Bats.

Bats have attracted global attention because of their zoonotic association with severe acute respiratory syndrome coronavirus (SARS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Previous and ongoing studies have predominantly focused on bat-borne viruses; however, the prevalence or abundance of bat-borne pathogenic bacteria and their potential public health significance have largely been neglected. For the first time, this study used both metataxonomics (16S rRNA marker gene sequencing) and culturomics (traditional culture methods) to systematically evaluate the potential public health significance of bat fecal pathogenic bacteria.

sp. nov. and sp. nov., isolated from bat faeces.

Four mesophilic actinobacteria (HY002, HY442, HY366 and HY285) isolated from the faeces of bats collected in southern China were found to be strictly aerobic, non-motile, rod-shaped, oxidase-negative, Gram-stain-positive and catalase-positive. Strains HY002 and HY366 contained -diaminopimelic acid as the diagnostic diamino acid and MK-9(H) the sole respiratory quinone. Arabinose, galactose and ribose were detected in the whole-cell hydrolysates of both type strains.

sp. nov., sp. nov. and sp. nov., isolated from the intestinal contents of .

Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157/zg-ZUI40, zg-ZUI222/zg-ZUI199 and zg-ZUI188/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C.