The strongest resistance of Staphylococcus aureus to erythromycin is caused by decreasing uptake of the antibiotic into the cells.

The consequence of excessive use of macrolides is a high occurrence of mechanisms responsible for resistance to these drugs. Of 97 erythromycin-resistant bacterial strains gathered in the Wrocław area in Poland, 60% exhibited very high resistance, and those with the inducible MLS(B) (macrolide-lincosamide-streptogramin B) resistance phenotype predominated. Direct genetic investigation revealed that the erm genes coding for ribosomal methylases are the most frequently occurring erythromycin resistance-determining genes.

[Antibodies to Klebsiella O-antigens in patients with seronegative spondyloarthropaties].

Objectives: The pathogenesis of seronegative spondyloarthropathies is still unknown. A microbial etiology has been suggested. The aim of the study was to analyze the antibodies against Klebsiella O-antigens in serum of patients with seronegative spondyloarthropathies.

Penetration of a selected antibiotic and antiseptic into a biofilm formed on orthopedic steel implants.

Background: The aim of the study was to determine the impact of octenidine hydrochloride and gentamicin on bacterial survival and reduction of biofilms formed on orthopaedic metal implants.

Material And Methods: We studied metal orthopaedic components (screws, nails, fragments of wires used in Ilizarov devices) and a bone sequester. The presence and intensity of biofilm formation on the medical biomaterials was determined using the method of Richards et al.

Major structural proteins of type 1 and type 3 Klebsiella fimbriae are effective protein carriers and immunogens in conjugates as revealed from their immunochemical characterization.

Fimbriae are filamentous structures present on the cell surface of many bacteria, including genus Klebsiella. The use of fimbriae as protein carriers in conjugates may allow to formulate effective multivalent vaccines and suitable diagnostics. However, the evidences have been reported that fimbriae may enhance the inflammatory response.

[Adhesion dependence of coagulase-negative staphylococci to biomaterials and PIA polysaccharide synthesis on icaADBC operon presence].

Both Staphylococcus epidermidis and Staphylococcus haemolyticus are important causes of infections associated with catheters and other medical devices. This infections result in significant morbidity, mortality and economic cost. It has recently been shown that not only S.

[Risk of coagulase-negative staphylococci infections in catheterized patients due to adhesion and slime production].

Unlabelled: Coagulase-negative staphylococci are important causes of infections associated with biomaterials, e.g. catheters.

High level of resistance to metronidazole and clarithromycin in Helicobacter pylori isolated from pediatric patients in Poland (1997-2001).

Resistance to metronidazole (Met), clarithromycin (Cla) and amoxycillin (Amo) was examined using H. pylori isolates from child patients before and after treatment in the period 1997-2001. The rate of Met and Cla resistance before treatment was 35.

[Siderophores and hemolysins in iron aquisition by Acinetobacter genus].

In 60 strains of Acinetobacter genus isolated from clinical material belonging to species A. baumannii, A. haemolyticus, A.

[Adhesive properties and antibiotic resistance of Klebsiella strains isolated from gastrointestinal tracts of children hospitalised in Wroclaw nad Opole].

Gram negative Klebsiella bacilli present many pathogenic properties, which determine their ability to survive and rapid spreading in hospital environment. There are many factors responsible for the pathogenicity of Klebsiella strains: capsule, fimbriae, nonfimbrial adhesins, lipopolysaccharide of the cell wall and extracellular secreted exotoxins. Klebsiella strains are etiological agents of different nosocomial infections but also colonized gastrointestinal and respiratory tracts.

[Selected features determine pathogenicity of Staphylococcus haemolyticus].

The study have been done on S. haemolyticus strains isolated from patients hospitalized an Surgical Unit. Aim of the study was to determine pathogenic traits of S.

[Occurrence and characteristic of P-like adhesin among Klebsiella strains].

Colonisation and remaining of microorganism on mucus membrane of microorganism is tightly connected with adhesion mechanisms and determine the first step of physiological settlement of the organism or the first stage of clinically demonstrated infection. In Klebsiella rods there are known three types of fimbrial adhesins (type 1, 3 and KPF-28) and non-fimbrial adhesin CF29K. It is stated that Klebsiella strains adhesions are responsible for their adherence to the epithelial cells of both respiratory and urinary tracts and to intestine epithelium.

Helicobacter pylori antigens in stool specimens of gastritis children before and after treatment.

Background: Various testing methods are successfully applied to the diagnosis of Helicobacter pylori infection, but noninvasive techniques are still needed for therapeutic monitoring, especially in children. In the search for new noninvasive techniques for the diagnosis of H. pylori infection, the authors evaluated an enzyme immunoassay for the detection of H.

[The effect of culture conditions on expression of surface proteins from coagulase-negative staphylococci isolated from clinical material of newborns hospitalized in an intensive care unit].

A surface adhesion is a fundamental stage, in a pathogenesis of inflammations caused by coagulase-negative staphylococci, at which surface proteins take part. The proteins that were found at S. saprophyticus and ascribed to take part in polystiren plates adhesion: SSP-1 and SSP-2 could be an example.

[Appearance of Helicobacter pylori antigen in stool specimens of children with chronic gastritis].

Various examinations are successfully applied for the diagnosis of Helicobacter pylori infection, but non-invasive techniques are still required for the therapeutic monitoring after eradication therapy, especially in children. In the search for new non-invasive techniques to diagnose H. pylori infection, we evaluated an EIA for H.

Characterization of coagulase-negative staphylococci by primer-specific polymerase chain reaction and ribotyping.

OBJECTIVE: To analyze, by primer-specific polymerase chain reaction (PCR) and ribotyping, coagulase-negative staphylococci (CNS). METHODS: Forty-five clinical isolates of CNS were identified by the API ID32 STAPH system and ribotyping. Additionally, primer-specific PCR was evaluated for identification of clinical strains of Staphylococcus epidermidis.

[Surface properties of Lactobacillus strains. II. Adherence to tissue culture surfaces].

In the second part of this study, we evaluated the adherence properties of specific strains of Lactobacillus isolated from both the human vagina and gastrointestinal tracts. Lactobacilli taken from the vagina and GI tract were tested for their adherence to A431 vaginal tissue, and to CaCo-2 cells taken from the gastrointestinal tract. The Lactobacillus strains with the most marked adherence to the respective cell lines were examined under the electron microscope.

[Surface properties of Lactobacillus strains. I. Hemagglutination and hydrophobicity].

Bacteria possess many surface membrane properties, both mechanical and biochemical, that allow them to interact with their environment These properties may affect a host in either positive (beneficial) or negative (pathological) ways. All surface properties of bacteria are yet unknown therefore we attempt to increase our knowledge regarding specific strains of lactobacilli, by examining the known properties including hemagglutination and hydrophobicity. The purpose of this study was to evaluate the cell surface properties of certain strains of Lactobacillus.

[Antagonistic activity of Lactobacillus bacteria strains against anaerobic gastrointestinal tract pathogens (Helicobacter pylori, Campylobacter coli, Campylobacter jejuni, Clostridium difficile)].

Antagonistic activity of Lactobacillus strains has been known for some time. This property is connected with production of many active substances by lactobacilli e.g.

Neutralisation of cytotoxic vacuolating activity by serum antibodies of Helicobacter pylori-infected patients.

The study involved 196 H. pylori strains and 196 serum samples taken from the same patients. H.

Neutralisation of vacuolating activity of cytotoxin by serum antibodies of Helicobacter pylori infected patients.

The study involved 196 H. pylori strains and 196 serum samples taken from the same patients. H.

[Adhesins of Acinetobacter strains].

One of the important factors contributing to the pathogenicity of bacteria is the presence of adhesins on cell surface, which facilitate colonisation in the macroorganism. The presence and type of adhesins occurring in four species of the genus Acinetobacter: A. baumannii (184), A.

[Evaluation of bacteriological research data and laboratory symptoms of infection in the diagnosis of congenital and acquired infections].

The predisposition of the newborns to contract infections diseases is dependent upon the limited efficiency of their immune mechanisms. Congenital infections amount to 5.7% in the research material, and the acquired infections 1.

Relationship between antibody to cytotoxin and Helicobacter pylori infection.

Broth culture supernatants from 14 (34%) out of the 41 H. pylori strains tested, induced vacuolization in Intestine 407 cells in titers ranging from 1:2 to 1:64. 20% of H.

Helicobacter pylori in kidney allograft recipients: high prevalence of colonization and low incidence of active inflammatory lesions.

Since kidney transplant recipients are at enhanced risk for developing severe upper gastrointestinal disease and Helicobacter pylori (Hp) is an important pathogen in active gastritis and peptic ulcer, we performed gastroduodenoscopic examination, coupled with assessment of Hp colonization in 29 renal allograft recipients complaining of recurrent dyspepsia. Results were compared with those of 25 chronically hemodialyzed patients and 16 subjects free from renal disease, also suffering from upper gastrointestinal symptoms of similar severity. We found that while transplant recipients have had a high prevalence of Hp infection (62 vs.