Sebaceous gland differentiation. I. Separation, morphology and lipogenesis of isolated cells from the mouse preputial gland tumor.

Single cell suspensions have been prepared, by enzyme digestion, from the mouse preputial gland tumor and separated by flotation centrifugation into populations of different buoyant densities. These populations of cells have been shown by morphological, chemical and biochemical criteria to be in different stages of maturation. Some properties of the separated cells are described.

Ultrastructural changes and viral morphogenesis during the growth of the mouse preputial gland tumor ESR 586.

A study was undertaken to observe morphological changes and viral morphogenesis during the growth of the mouse preputial gland tumor ESR 586. The acinar cells of the normal preputial gland have an extensive agranular endoplasmic reticulum and Golgi apparatus and large lipid droplets. No viral particles are present.

Culture of cloned cells from the mouse preputial gland tumor ESR 586.

Cells from the mouse preputial gland tumor ESR 586 have been cultured and cloned. Trypsin-ethylenediaminetetraacetate was used to obtain single cells. The cells are grown in a modified CMRL-1415 medium supplemented with 10% fetal calf serum.

Demonstration of gap junctions by lanthanum in the vitamin A acid-treated skin tumor, keratoacanthoma.

In normal rabbit epidermis or in the untreated skin tumor, keratoacanthoma the usual cell junction is the desmosome. Gap junctions are very sparse. The extracellular tracer material, lanthanum nitrate was used to confirm the definite identification and increase of gap junctions in the vitamin A acid-treated keratoacanthoma.

Mucous metaplasia and gap junctions in the vitamin A acid-treated skin tumor, keratoacanthoma.

Desmosomes are the usual cell junctions found in normal rabbit epithelium as well as in the untreated keratoacanthoma. This study reports the finding of a second cell junction, the gap junction, when epithelium, normal or tumorous, is subjected to topical applications of vitamin A acid. The gap junction forms early in mucous metaplasia (after 2 days of application of vitamin A acid) and appears before the gross appearance of mucus.