Quantitative PCR as a tool to determine the reticuloendotheliosis virus-proviral load of fowl poxvirus.

Sequences of the reticuloendotheliosis virus (REV), an avian retrovirus, are integrated into the genome of fowl poxvirus (FPV). We developed and evaluated a quantitative multiplex real-time PCR (multiplex qPCR) assay to determine the REV-proviral load of FPV strains. Amplification efficiencies were 98.

Serologic response against fowl poxvirus and reticuloendotheliosis virus after experimental and natural infections of chickens with fowl poxvirus.

Two infection studies in chickens were done to investigate the humoral immune response against fowl poxvirus (FPV) and reticuloendotheliosis virus (REV) after intradermal infection with different passages of a field isolate and with the vaccine strain HP B. The field isolate in a low passage carried the near-full-length REV provirus and induced antibodies to REV, but not to FPV. The vaccine strain carried only remnants of the long terminal repeat and induced antibodies against FPV, but not against REV.

Some strains of serotype 4 fowl adenoviruses cause inclusion body hepatitis and hydropericardium syndrome in chickens.

Fowl adenoviruses were isolated and characterized from severe cases of hydropericardium syndrome in Ecuador and Pakistan. All were neutralized by antibodies against serotypes 4 and 11. Cross-neutralization tests and restriction enzyme analysis strengthen the classification as serotype 4 strains.

Growth analysis of adenoviruses isolated from pigeons in chicken cells and serological characterization of the isolates.

The susceptibility of chicken embryo liver (CEL) and chicken kidney (CK) cells to eight adenoviruses isolated from different pigeons were investigated. Isolation and propagation was most successful in CEL cells. The cytopathic effect, i.

Protection and virus shedding of falcons vaccinated against highly pathogenic avian influenza A virus (H5N1).

Because fatal infections with highly pathogenic avian influenza A (HPAI) virus subtype H5N1 have been reported in birds of prey, we sought to determine detailed information about the birds' susceptibility and protection after vaccination. Ten falcons vaccinated with an inactivated influenza virus (H5N2) vaccine seroconverted. We then challenged 5 vaccinated and 5 nonvaccinated falcons with HPAI (H5N1).

Avipoxvirus infection in a collection of captive stone curlews (Burhinus oedicnemus).

A natural outbreak of avipoxvirus occurred in recently purchased stone curlews (Burhinus oedicnemus) at a breeding farm and subsequently spread to other stone curlews residing at the farm. The initial outbreak was characterized by mild vesicular skin lesions on the legs, which then developed crusts and bled. The overall morbidity rate was 100%, but none of the birds died, and all recovered without complication.

Adenovirus of psittacine birds: investigations on isolation and development of a real-time polymerase chain reaction for specific detection.

Liver samples of psittacine birds with a histological suspicion of an adenovirus infection, confirmed by electron microscopy examination, were subjected to virus isolation attempts using a heterologous cell culture system and a homologous cell culture system in the form of chicken embryo liver cells and psittacine embryo fibroblasts, respectively. Whereas isolation in chicken embryo liver cells failed, virus was isolated successfully in the psittacine embryo fibroblasts cell culture system. Molecular investigations identified the virus as a specific psittacine adenovirus (PsAdV).

Investigations on the aetiology of pinching off syndrome in four white-tailed sea eagles (Haliaeetus albicilla) from Germany.

The purpose of this study was to investigate the aetiology of the pinching off syndrome (POS), a generalized feather abnormality affecting free-living nestling of the white-tailed sea eagle (Haliaeetus albicilla) in Europe. For the first time, extensive clinical, haematological, biochemical, virological, bacteriological, nutritional, histopathological, parasitological and electron microscopical examinations were performed on three females and one male suffering from POS. Early and increased cytokeratin formation at the base of regenerating feathers and their follicle was observed in affected birds.

Investigation into the seroprevalence of falcon herpesvirus antibodies in raptors in the UK using virus neutralization tests and different herpesvirus isolates.

Increasing numbers of reports of clinical falcon herpesvirus infection (Falconid herpesvirus-1; FHV-1) have been seen in the UK since 1996. The aim of this epidemiological study was to investigate the seroprevalence of FHV-1 and owl herpesvirus (Strigid herpesvirus-1; StHV-1) infection in the UK, using virus neutralization tests, and to evaluate the prevalence of herpesvirus infection in captive and wild raptor populations. The results, using the English FHV-1 CVL 32/93 isolate, revealed a seroprevalence of 3.

Antibodies to adenoviruses in free-living common buzzards from Germany.

One hundred sixty-seven plasma samples of free-living birds of prey from Berlin and Brandenburg State (Germany) were tested for antibodies against avian adenovirus (FAV, group I) using agar gel precipitation test. Antibodies to FAV were detected in seven (4%) of 167 total samples. The positive samples originated only from common buzzards (Buteo buteo; seven [12%] of 59).

Presence of avian pneumovirus type A in continental Europe during the 1980s.

Three isolates of avian pneumovirus (APV) were isolated in Germany during 1987 and 1988 from turkeys with clinical signs of turkey rhinotracheitis and one was isolated during 1990 from a broiler breeder flock with typical signs of swollen head syndrome. The isolates were typed using type-specific reverse transcriptase-polymerase chain reactions. The three isolates from turkeys were identified as type A, while the isolate from the broiler breeders was type B.

[Epizootiology of fowls adenoviruses].

Fowl adenoviruses of the serotype 4 from Germany were characterised by restriction enzyme analysis in comparison to isolates from Asia, South America and the FAV4 reference strain KR5. Only strain Da60 which was isolated from a psittacine aviary was identical with the reference strain KR5. None of the isolates was identical with the highly pathogenic strains from India and Ecuador.

Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium.

Serology, restriction enzyme analysis and polymerase chain reactions were used to classify a total of 12 fowl adenoviruses (FAV) isolated from clinical cases of infectious hydropericardium from field outbreaks in seven countries in Asia and America. All isolates belonged to FAV serotype 4. Two isolates were contaminated with avian adeno-associated virus and one of them also contained FAV1.

Characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in Chile.

Three fowl adenovirus (FAV) isolates (341, 344, and 215) obtained during 1996-97 from field outbreaks of inclusion body hepatitis/hydropericardium syndrome (IBH/HPS) affecting broilers and broiler breeders in Chile were characterized by virus neutralization tests (VNTs) and restriction enzyme analysis of a DNA fragment. Furthermore, the pathologic characteristics of one of these FAV isolates (FAV 341) was studied in experimentally infected chickens. The VNTs conducted with isolates 341 and 344 against reference strains and antisera belonging to each of 12 FAV serotypes demonstrated a close antigenic relationship with strain KR5 of the FAV serotype 4.

[Adenovirus infections in psittacines].

Diagnostic investigations were carried out in a fatal disease of several African Grey Parrots (Psittacus erithacus) and Cape Parrots (Poicephalus robustus) in a large breeding plant. Electron microscopically examination of liver and intestine, the organs with the most prominent pathomorphological changes, regularly revealed adenoviruses. Necrotizing hepatitis and catarrhal to haemorrhagic enteritis dominated on histopathological examination.

Isolation of fowl adenoviruses serotype 4 from pigeons with hepatic necrosis.

Nine homogenized livers were taken to isolate the causative agent of adenovirus type I and type II infections in pigeons. The samples were passaged up to four times on primary chicken embryo hepatocytes. Adenoviruses were isolated from all of the six type II but none of the three type I infections.

Identification and characterization of penton base and pVIII protein of egg drop syndrome virus.

The nucleotide sequence and location of the penton base and pVIII genes of the egg drop syndrome (EDS) virus an avian adenovirus, were determined. The penton base gene is located at 34.8-38.