Phosphoprotein dynamics of interacting T cells and tumor cells by HySic.

Functional interactions between cytotoxic T cells and tumor cells are central to anti-cancer immunity. However, our understanding of the proteins involved is limited. Here, we present HySic (hybrid quantification of stable isotope labeling by amino acids in cell culture [SILAC]-labeled interacting cells) as a method to quantify protein and phosphorylation dynamics between and within physically interacting cells.

Hypoxia Triggers TAZ Phosphorylation in Basal A Triple Negative Breast Cancer Cells.

Hypoxia and HIF signaling drive cancer progression and therapy resistance and have been demonstrated in breast cancer. To what extent breast cancer subtypes differ in their response to hypoxia has not been resolved. Here, we show that hypoxia similarly triggers HIF1 stabilization in luminal and basal A triple negative breast cancer cells and we use high throughput targeted RNA sequencing to analyze its effects on gene expression in these subtypes.

Correlation between ultrasonic and radiographic imaging of developmental dysplasia of the hip.

Purpose: The correlation between the degree of developmental hip dysplasia (DDH) measured on ultrasound images compared with that measured on radiographs is not clear. Most studies have compared ultrasonography (US) and radiographic images made at different times of follow-up. In this study the correlation between US images and radiographs of the hip made on the same day was evaluated.

The IFITM5 mutation c.-14C > T results in an elongated transcript expressed in human bone; and causes varying phenotypic severity of osteogenesis imperfecta type V.

Background: The genetic mutation resulting in osteogenesis imperfecta (OI) type V was recently characterised as a single point mutation (c.-14C > T) in the 5' untranslated region (UTR) of IFITM5, a gene encoding a transmembrane protein with expression restricted to skeletal tissue. This mutation creates an alternative start codon and has been shown in a eukaryotic cell line to result in a longer variant of IFITM5, but its expression has not previously been demonstrated in bone from a patient with OI type V.

Assessment of the relationship between joint laxity and migration of the hip in children with Down syndrome.

Purpose: The aim of this cross-sectional cohort study is to describe the incidence of joint laxity and the correlation between joint laxity and radiological migration of the hip in children with Down syndrome.

Methods: Sixty-five children (2-19 years) with Down's syndrome were examined for joint laxity. For each subject, laxity scores for joints were carried out with the Bulbena method.

Decrease in outpatient department visits and operative interventions due to bisphosphonates in children with osteogenesis imperfecta.

Introduction: Bisphosphonates are currently the medical treatment most often used in children with osteogenesis imperfecta (OI). The purpose of this retrospective pre-post study was to evaluate the efficacy of treatment with bisphosphonates. We measured the effect by evaluating the number of outpatient department consultations and operative interventions before and after treatment with bisphosphonates in children with OI.

A Dutch guideline for the treatment of scoliosis in neuromuscular disorders.

Background: Children with neuromuscular disorders with a progressive muscle weakness such as Duchenne Muscular Dystrophy and Spinal Muscular Atrophy frequently develop a progressive scoliosis. A severe scoliosis compromises respiratory function and makes sitting more difficult. Spinal surgery is considered the primary treatment option for correcting severe scoliosis in neuromuscular disorders.

Spinal fusion in children with spina bifida: influence on ambulation level and functional abilities.

The aim of this study was to determine the influence of spinal fusion on ambulation and functional abilities in children with spina bifida for whom early mobilization was stimulated. Ten children (three males and seven females) with myelomeningocele were prospectively followed. Their mean age at operation was 9.

Osteogenesis imperfecta in childhood: effects of spondylodesis on functional ability, ambulation and perceived competence.

We studied the effects of spondylodesis on spinal curvature, functional outcome, level of ambulation and perceived competence in 11 children with osteogenesis imperfecta (OI). Mean age at surgical intervention was 13.1 years (SD 2.

Osteogenesis imperfecta in childhood: MR imaging of basilar impression.

Objective: To determine on radiographs the presence of Basilar Impression (BI) in children with Osteogenesis Imperfecta (OI). To confirm this sign and altered geometrical relationships of the craniocervical junction in course of time with magnetic resonance imaging (MRI).

Methods And Patients: In a cohort study of 130 patients with OI (OI type I: 85; OI type III: 21; OI type IV: 24) lateral radiographs of the skull and cervical spine were made in a standardised way.

Hemi-epiphysiodesis for unclassified congenital scoliosis: immediate results and mid-term follow-up.

Complex spinal anomalies involved in congenital scoliosis consist of a jumble of vertebral defects. Progressive scoliotic curves are frequently encountered in these patients. We evaluated the results of hemi-epiphysiodesis for this patient population.

[Spondylodiscitis in 3 children; differential diagnosis and treatment].

Three children, a 4.5-year-old boy and two girls aged 21 months and 10 years respectively, had for several weeks to months experienced lower back pain or walking problems, two of them had an elevated sedimentation and leucocytosis. The MRI scan revealed a narrowing of the lumbal disk.

[Diagnostic image (42). Solitary bone cyst of the left calcaneus].

A 9-year-old boy presented with a painful left heel. A plain radiograph showed a lytic lesion with a benign appearance, with a simple bone cyst or an intraosseous lipoma as the most likely differential diagnostic possibilities. MRI demonstrated a simple bone cyst.

Operative treatment of severe scoliosis in osteogenesis imperfecta: results of 20 patients after halo traction and posterior spondylodesis with instrumentation.

Correction and stabilisation of the scoliotic spine in osteogenesis imperfecta is difficult. The optimal technique has yet to be determined, since no large series in which a single procedure has been carried out by a single surgeon using a single protocol has yet been described. The charts of 20 patients with osteogenesis imperfecta who had undergone halo gravity traction (HGT) and a posterior spondylodesis with Cotrel-Dubousset (n = 18) or Harrington (n = 2) instrumentation were reviewed.

Neuromuscular scoliosis: clinical evaluation pre- and postoperative.

Neuromuscular scoliosis concerns a wide variety of disorders, all with a different approach to surgical intervention. The impact of these disorders on a patient is discussed in terms of pulmonary, cardiac, bowel and bladder function and complications in the perioperative and postoperative period such as gastric ulcers, pathologic fractures, wound healing, and nutrition is described. These difficult patients need a multidisciplinary approach.

Instrumentation for correction and fixation of scoliosis in fibrous dysplasia of the thoracolumbar spine.

A case report is presented in which correction of a thoracolumbar scoliosis, due to extensive fibrous dysplasia, is achieved using segmental spinal instrumentation with a 6-year follow-up. Pseudoarthrosis developed due to insufficient hook fixation at the sacrum.

[One hundred years of orthopedics in the Netherlands. VIII. Pediatric orthopedics].

Orthopaedic disorders in children differ in type from those in adults: most frequent are congenital anomalies and disorders of growth and development. The special nature and relative rarity of these conditions justify the separate development of this branch of the discipline. Fractures almost always heal normally after closed reduction and immobilization in a plaster cast; fractures close to epiphyseal discs and in joints require special attention.

Surgical treatment of wrist deformity in hereditary multiple exostosis.

Wrist deformity represents a unique problematic entity in patients with Hereditary Multiple Exostosis (HME). We report our experience in the treatment of wrist deformities due to HME using three surgical procedures and the outcome of 12 wrist surgical corrections by comparing preoperative, postoperative and last follow-up radiographic values of the carpal slip, radial articular angle and ulnar shortening. Eight out of 12 forearms did show a postoperative improvement of the radiographic parameters, although recurrence of the deformity occurred frequently.

The benefits of school screening for scoliosis in the central part of The Netherlands.

The Netherlands has well-organized school health services, and children are assessed on a regular basis for scoliosis among other disturbances and pathologies. The purpose of this study was to assess the benefits of an annual screening programme for scoliosis in the Netherlands. Three cohorts of 10,000 children sampled at 10, 12 and 14 years of age, respectively, were followed for 3 years.

School screening for scoliosis: the value of quantitative measurement.

Several studies advocate quantification of the bending test or performing surface topography to reduce the referral rate and to increase the specificity of the bending test in screening for scoliosis. Within the framework of a school screening project all children with a positive bending test were reexamined with measurement of rib hump height, angle of trunk rotation and moiré topography. In the period 1983-1986, out of three cohorts of 10,000 children of 10, 12 and 14 years of age, 3,069 were reexamined, of whom 1931 again tested positive (63%).

Spinal rotation meter: development and comparison of a new device.

Quantification of the bending test became necessary within a large school screening for scoliosis, which started in 1983 in the central part of the Netherlands. Measurement of the angle of trunk rotation appeared to be the easiest method for the school doctors involved. Since at that time no device was commercially available, a device was developed to permit an easy and reliable angle measurement.

Parameter variation in stable scoliosis.

The reliability of scoliosis parameters such as the Cobb angle, the angle of trunk rotation and moir e topography is known from several publications. These studies concern intra- and interobserver variations; they are in general of transversal nature, which means the measurements were performed on the same subject within a short time period. From clinical experience it appears that these variations may be greater when scoliosis patients are followed over several years.

Variation in Cobb angle measurements in scoliosis.

In order to determine the reliability of the Cobb angle measurement as it is used in the clinical management of scoliosis, a methodological survey was carried out. In the measurement of a Cobb angle two phases can be distinguished: (a) the production of a spinal radiograph and (b) the measurement of the angle itself. In respect of the first phase, the variation in production of the radiographs was calculated on Cobb angle measurements made by one investigator on serial radiographs of patients who underwent spinal fusion for scoliosis and therefore had a fixed spinal curvature.