Publications by authors named "Prucha J"

The aim of the present study was to investigate the effect of laser therapy on leukopoiesis recovery after irradiation with ionizing radiation. A dose of ionizing radiation was used that induced the hematological form of radiation sickness, reducing the number of blood cells. Subsequently, mice were treated with non-ionizing laser radiation.

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Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz.

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The present study aimed to evaluate the characteristic influence of physical therapeutic procedures of vacuum-compression therapy (VCT) on microvascular perfusion (MiP) and macrovascular perfusion (MaP) of the lower limb in diabetic patients. A sample of nine patients with a medical history of type 2 diabetes was used for the purpose of this study. Most of the subjects' medical conditions included venous and neurological complications of the lower limb, whereas the rest of the subjects entered the treatment due to injury recovery or their phlebological disease.

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Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have not yet been fully understood. The detailed quantification of LF EMF effects on the angiogenesis were analysed in our experiments by using cultured human and mouse endothelial cells. Two types of fields were used in the tests as follows: the LF EMF with rectangular pulses, 340-microsecond mode at a frequency of 72 Hz and peak intensity 4 mT, and the LF EMF with sinusoidal alternating waveform 5 000 Hz, amplitude-modulated by means of a special interference spectrum mode set to a frequency linear sweep from 1 to 100 Hz for 6 s and from 100 Hz to 1 Hz return also for 6 s, swing period of 12 second.

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Nowadays, a high-induction magnetic stimulation is starting to be increasingly applied as a biophysical stimulation in the conservative treatment of the degenerative locomotor system diseases. These are mainly in correlation with the changes in soft tissue elasticity, which should be positively influenced by the flow-induced electrical currents of high current density during high-induction magnetic stimulation. This assumption was verified within the interventional and prospective study using the ultrasound elastography.

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can cause bloodstream infections associated with infective endocarditis (IE) and disseminated intravascular coagulopathy (DIC). Both complications involve platelets. In view of an increasing number of antibiotic-resistant strains, new approaches to control systemic infection are gaining importance.

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Exposure to repetitive low-frequency electromagnetic field (LF-EMF) shows promise as a non-invasive approach to treat various sensory and neurological disorders. Despite considerable progress in the development of modern stimulation devices, there is a limited understanding of the mechanisms underlying their biological effects and potential targets at the cellular level. A significant impact of electromagnetic field on voltage-gated calcium channels and downstream signalling pathways has been convincingly demonstrated in many distinct cell types.

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Unlabelled: Mycobacterium tuberculosis secretes several hundred proteins; many of which elicit immune responses. As a result, many of these proteins have been explored for their potential as diagnostic and vaccine candidates. Of these, the Antigen 85 complex proteins, represented by Antigen85 A, B, and C, are the most studied from the mycobacterial secretome.

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Background: The R6K replicon is one of the best studied bacterial plasmid replicons. Replication of the R6K plasmid and derivatives harboring its γ origin of replication (ori(R6Kγ)) is dependent on the pir gene-encoded π protein. Originally encoded by R6K, this protein is usually provided in trans in hosts engineered to support replication of plasmids harboring ori(R6Kγ).

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Salmonella is a multi-faceted problem that must be attacked on many fronts. The results of Food Safety and Inspection Service's Puerto Rico Bacterial Control Project confirmed that there is no "silver bullet." The project showed that a combination of management commitment, process control, and specific interventions at key control points can bring about significant improvements in the bacterial quality of fresh poultry.

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In June and September 1988, the USDA Food Safety and Inspection Service sampled raw chicken carcasses at a federally inspected slaughter establishment in Puerto Rico to determine the effects of changing the scalding equipment on bacterial contents of raw poultry products. The scalding equipment was changed to a countercurrent configuration, with a postscald hot-water rinse cabinet that sprayed carcasses as they exited the scalder. Analysis of 250 carcass-rinse samples collected at preevisceration, prechill, and postchill sites over 7 days indicated that carcasses had mean aerobe plate counts of log(10)3.

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In March 1989, the USDA Food Safety and Inspection Service sampled raw chicken carcasses and giblets at a federally inspected slaughter establishment in Puerto Rico to determine the effects of adding chlorine to carcass and giblet chill water on bacterial contents of raw poultry products. Over four 8-hour workdays, 200 carcass rinse samples were collected at 3 sites in the establishment; 39 giblet rinse samples were collected at 1 site. Analyses of the carcass rinse samples indicated that carcasses had average aerobe plate counts of log10 3.

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The USDA Food Safety and Inspection Service determined populations of bacteria on poultry during processing at a slaughter plant in Puerto Rico in November and December 1987. The plant was selected because of its management's willingness to support important changes in equipment and processing procedures. The plant was representative of modern slaughter facilities.

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The study demonstrates that the agents used for dishwashing and in the central laundry are already slightly contamined with halogenated hydrocarbons and formaldehyde. Analyses of the washing lyes yield considerably higher concentrations of halogenated hydrocarbons. Especially in the dishwater a relatively high concentration of trichloromethane (chloroform) was detected.

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This study describes the contamination of human adipose tissue with six selected polychlorinated biphenyls (PCB) known as "indicator congeners", with PCB no. 49 and the mixed inducer PCB nos. 156, 170, and 189.

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[Heavy metal content of the children's hair].

Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med

November 1987

In the framework of the epidemiological group-diagnostic studies in the industrial areas Rhine-Main (Wiesbaden and Frankfurt) with a relatively high air pollution as well as in two control areas (Freiburg and Starnberg) with lower air pollution, hair samples from more than 2500 ten-year-old children were taken. After an appropriate washing process (removal of the exogenic load) and wet incineration, the hair samples were examined by AAS- for their heavy metal content (Cd, Pb, Fe and Zn). The evaluation of the results over the time of the examination from 1982 until 1986 shows that the hair samples from children in the burdened area Rhine-Main with high air pollution have higher lead as well as cadmium values than those of the control area Freiburg with the bordering region of the Black Forest.

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[Environmental characterization: weather and climate].

Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med

November 1987

In order to characterize the weather and the climate in the area of industrial Rhine-Main area (Wiesbaden and Frankfurt) and in the two control areas (Freiburg with the bordering higher region of the Black Forest and the Starnberg area), relevant measuring data was provided by the German Meteorological Service, Centre of the Medicometeorological Research Establishment, Freiburg. The monthly as well as yearly climatic values of the years 1982-1985 were employed for the following stations: Frankfurt Airport; Wiesbaden South; Freiburg Meteorological Office; Munich Airport Riem which are representative for our areas under examination. For the characterization of the climate in the study areas the following parameters were used: temperature degrees C; relative humidity of the air in %; amount of rainfall in mm; duration of sunshine; wind distribution.

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[Environmental characterization: air pollution].

Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med

November 1987

During the years of the epidemiological group-diagnostic examination (1982-1986) it was attempted to characterize the atmospheric air pollution in the industrial Rhine-Main area (Wiesbaden and Frankfurt) and, in comparison, in two control areas (Freiburg with the adjacent High Black Forest and the Starnberg area). For this the measurements of the air measuring stations of the network for emissions of the German Umweltbundesamt (Federal Environmental Protection Agency) at Berlin and Frankfurt were used and well as those of the Hessische Landesanstalt für Umwelt, Wiesbaden (Hessian Office for Environmental Protection); Landesanstalt für Umweltschutz Baden-Württemberg, Karlsruhe (Regional Office for Environmental Protection of Baden-Württemberg); Bayerische Landesanstalt für Umweltschutz (Bavarian Office for Environmental Protection, Munich), were used. The comparison of the different measuring components used shows the pollutant concentration in the air at the measuring stations in Wiesbaden and Frankfurt attains the highest values.

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Two of three persons who ate lunch together became ill with symptoms characteristic of botulism. One died before botulism was suspected and before specimens could be collected for laboratory testing, but a serum specimen from the other patient, who survived, yielded botulinal toxin, type A. The third person remained asymptomatic, but Clostridium botulinum type A was cultured from his stool.

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