[Minimally invasive operations for varicose disease of lower extremities].

The authors discuss specific using minimally invasive surgical technique of ablation of the saphenous vein trunks by a modified Oesch PIN stripper method proposed by the authors. An experience with surgical treatment of 245 patients with varicose veins using the same modified technique is analyzed. Postoperative complications and medium-term results are evaluated.

[Venectomy under local tumescent anaesthesia].

The authors have analysed the findings of the examination and outcomes of surgical management of 142 patients with lower limb varicosity (a total of 160 lower extremities). Of theses, there were 52 (36.6%) men, and 90 (63.

[Biological and genetic characterization of strains of the pathogen of legionellosis, isolated from water on Russian territory].

As the result of the study of the spread of Legionella in different regions of Russia, 69 cultures were isolated from different water systems. After serotyping most of these strains (85%) were identified as L. pneumophila, serogroups 1 and 6.

[Analysis of genomic polymorphism in leptospira by polymerase chain reaction with random primers].

A test system for genetic typing of Leptospirae is developed, based on the polymerase chain reaction (PCR) with arbitrary primers. Thirteen strains of 4 Leptospira species were examined: L. interrogans, L.

[The theoretical and practical aspects of the nonspecific prevention of infectious diseases among the troops].

As possible alternative to a complex of sanitary-hygienic measures, directed on the infectious diseases prevention, not specific prophylaxis with the aid of immunomodulative preparations is offered. Prospects of the immunomodulative preparations application is defined by transition to popularized epidemiological thinking planned in modern conditions. Strategy of total and selective not specific prophylaxis, results of own researches on estimation of preventive efficiency of dibazolum and prodigiosanum during acute respiratory disease and virus hepatitis A are described.

[Genomic polymorphism in Mycobacterium tuberculosis strains].

Different genomic fingerprinting techniques (universal probes, such as rRNA genes, phage M13 DNA, IS 6110 probe) have been used to investigate the genomic polymorphism of Mycobacterium tuberculosis strains isolated in different geographical regions of Russia and in some CIS countries. As shown with the use of these techniques and a specially developed PCR-mediated system for genetic typing, M.tuberculosis strains are genotypically heterogeneous in regions with a sporadic level of tuberculosis morbidity and genotypically homogeneous in regions with elevated morbidity and mortality levels.

[Molecular genetic approaches to the study of the persistence of pathogenic Mycoplasma].

The inhibition of the amplification of different regions of Mycoplasma pneumonia genome, depending on the conditions of cultivation, was observed with the use of polymerase chain reaction. A protein, stably associated with DNA, is responsible for this inhibitory effect. When selectively associated with different sites of DNA, the protein seems to be capable to inhibit the expression of genes, encoding pathogenicity factors of mycoplasmas and thus promoting their transformation into persistent forms.

[The laboratory diagnosis of mycoplasmosis and ureaplasmosis in urological and gynecological patients].

The survey of 630 patients with urogenital pathology, habitual miscarriage and sterility revealed that they were mostly (91-100%) infected with M. hominis and/or U.urealyticum.

[Urogenital mycoplasmosis and Mycoplasma carriage during pregnancy and in inflammatory processes of the genitalia in workers in the electronics industry].

To find out the spread of urogenital Mycoplasma carriership urogenital mycoplasmosis (UGM) among women living and working under similar conditions and making up risk groups with respect to these infections, pregnant women, gynecological patients and clinically healthy women were specially surveyed. As revealed in this survey, UGM and Mycoplasma carriership were found in clinically healthy female workers significantly more often than in other similar groups of the same region. In the group of pregnant women the occurrence of Mycoplasma carriership and UGM reached 90%.

[Selective inhibition of DNA amplification in nonadhesive cultures of Mycoplasma pneumoniae].

Inhibition of amplification of various genome regions of Mycoplasma pneumoniae was observed in the polymerase chain reaction, and was dependent on cultivation conditions. A protein stably associated with DNA is responsible for the inhibitory effect. It is assumed that when the protein selectively associates with separate DNA regions, it can inhibit genes encoding pathogenicity factors, thus promoting mycoplasma transformation into persistent variants.

[The isolation and partial characterization of the cell wall proteins of Listeria monocytogenes].

The preparative scheme for the purification of proteins with molecular weights of 39 and 79 kD, obtained from L. monocytogenes membrane fractions, has been developed. This technology included the cultivation of bacteria in heart-brain broth, isolation of bacterial membranes, the extraction of their components with Triton-X-100 and chromatography on Superose columns.

[Identification of tuberculosis pathogens in clinical material using the polymerase chain reaction].

The authors examined the possibility of detecting M. tuberculosis cells in various types of diagnostic material (sputum, blood, bone marrow, bronchoalveolar lavage fluid) from tuberculosis patients using polymerase chain reaction (PCR). The developed PCR-based test systems helped detect M.

[Cloning and expression of the phosphatidylinositol-specific phospholipase C gene from Listeria monocytogenes].

The gene for phosphatidylinositol-specific phospholipase C (PI-PLC) of Listeria monocytogenes has been cloned and shown to be expressed in Escherichia coli cells from own as well as from the lactose gene promoter. The recombinant plasmid has been constructed on the basis of pRIT2T vector and carries the hybrid gone. 3-end of which is a fragment of protein A gene of Staphylococcus aureus.

[Is sarcoidosis a chronic persistent infection?].

The study carried out with the use of microbiological diagnostic methods has revealed that in 67% of cases specimens obtained from sarcoidosis patients for analysis contain different forms of mycobacteria (typical Mycobacterium tuberculosis and granular forms of mycobacteria). The content of typical and granular forms of mycobacteria detected in diagnostic specimens has been shown to differ, depending on the clinical form of sarcoidosis: as a rule, in cases of the sluggish course of sarcoidosis granular forms of mycobacteria are detected, while during the exacerbation of the disease and in cases of the acute course of newly diagnosed sarcoidosis the proportion of typical M.tuberculosis increases.

[The use of an amplification test system for detecting persistent mycoplasmas].

A DNA amplification test system for the detection of Mycoplasma fermentans in clinical specimens was developed. The system was used for the analysis of biological specimens obtained from experimentally infected animals. The infective agent could be detected during the whole period of observation (6 months).

[The effect of changes in the cultivation conditions on the amplification of different Mycoplasma pneumoniae genes].

In experiments carried out with the use of the polymerase chain reaction the inhibition of the amplification of several regions of M. pneumoniae genome, depending on the conditions of their cultivation, has been observed. We suggest that protein, selectively binding with individual sites of DNA, is capable of inhibiting the expression of genes coding pathogenicity factors and thus contributes to the transformation of mycoplasmas into persistent forms.

[The purification and characteristics of listeriolysin O from Listeria monocytogenes].

A scheme of the purification of listeriolysin O produced by L. monocytogenes strain NCTC 7973 was developed. The isolation procedure included the cultivation of the bacteria in heart-brain broth, the concentration of culture liquid free of bacteria with ammonium sulfate, cation exchange chromatography on a column packed with CM-Sepharose and Mono S, gel chromatography on a column packed with Superose 12.

[Use of the polymerase chain reaction for studying the processes of Salmonella typhimurium cells to an noncultivated state].

The possibility to identify noncultivating forms of Salmonella by the polymerase chain reaction (PCR) has been shown. To do it the technique for Salmonella identification was elaborated, based on amplification of a 500 bp fragment of araC gene. Time course of populations of two Salmonella typhimurium strains during prolonged incubation in water was studied by the techniques of serial dilutions on solid nutrient media, acridine orange staining, and PCR.

[Species-specific detection of Listeria monocytogenes by directed DNA amplification].

A highly sensitive species-specific test system which allows detection Listeria monocytogenes in animal tissues is elaborated using the principle of a polymerase chain reaction. The potentialities of its application were studied in the conditions of experimental infection of animals. It is shown that while the procedure of detection is relatively simple and fast, the test system is as sensitive and specific as traditional microbiological methods.

[Test-systems of polymerase chain reaction for determining the tuberculosis pathogen].

Two polymerase chain reaction-based test systems were used to identify Mycobacteria tuberculosis by using a clinical material for practical health purposes. The former test system strictly specifically enables M. tuberculosis to be detected.