[The antiradical activity of plant extracts and healthful preventive combinations of these exrtacts with the phospholipid complex].

Using the chemiluminescence method, the effective concentration of antioxidants (AO) and its reactivity toward peroxyl radicals (ARA, the k7 constant) have been measured for 13 plant extracts. In fact all extracts demonstrated ARA higher than ionol. Larix dahurica, Hypericum perforatum, Potentilla fruticosa, Aronia melanocarpa and Rhaponticum carthamoides extracts showed the highest values of ARA.

[Anti-radical activity and oxidative stability of oils manufactured from flax, sesame and silybum seeds].

Comparative analysis of oils, prepared from flax, sesame and silybum seed composition, and adequate oil blends, prepared by mixing flax oil, sesame oil and silybum oil was performed. Antiradical activity was evaluated for the oils, both for their methanol-soluble phase and the fraction insoluble in methanol. DPPH radical scavenging activity expressed in Trolox equivalent antioxidant capacity.

[Antioxidant activity of vegetable oils with various omega-6/omega-3 fatty acids ratio].

Antioxidant activity and the oxidative stability were investigated in flax, sesame, silybum oils and oils with different omega-6/omega-3 fatty acid ratio. The content of antioxidants (AO) in crude oils and their reactivity towards peroxyl radicals were studied using kinetic method for addition of oil in a model reaction of cumol oxidation. There were correlations between PUFA/omega-9 and thermal stability (50 degrees C); between gamma-tocopherol content and resistantance to oxidative changes after storage at (10 +/- 2) degrees C for 6 months.

[Antiradical activity and resistance of flaxseed oil enriched with the antioxidants to oxidative changes].

Using the chemiluminescence technique, the effective concentration of antioxidants (AO) and its reactivity towards peroxyl radicals (constant k7) have been measured for the oil "Ubicato-flax" (biological active supplement) and flaxseed oil, enriched with Aronia melanocarpa extract. Ubicato-flax exhibited the highest antiradical activity but it was less resistant to oxidative changes as compared with flaxseed oil + extract. Ubicato oil produced on basis of natural AO exhibited higher antiradical activity in comparison with Ubicato oil produced on basis of the chemical synthetic AO.

[Biological effects of the soybean phospholipids].

Soyabean phospholipids, particularly commercial lecithin, are now widely used as biological active food additives. Mechanisms of their activities are based mainly on their similarity with ow phospholipids of biomembranes and blood lipoproteins. The similarity allows the inclusion of plant phospholipids into these structures and promotes prevention of number of pathological processes.

[Biological activity of linseed oil as the source of omega-3 alpha-linolenic acid].

Flaxseed oil is the most abundant plant source of omega-3 fatty acid, alpha-linolenic acid omega-3. This review focuses on the biological effects of dietary alpha-linolenic acid (ALA) compared with long-chain omega-3 derivatives. ALA is not equivalent in its biological effects to the long-chain omega-3 fatty acids found in marine fish oils.

[Antioxidant properties of a leaf extract from Aronia (Aronia melanocarba) containing proanthocyanidins].

Antioxidant capacity of a procyanidin-containing extract from Aronia melanocarpa leaves has been studied in vitro and in vivo. Using the chemiluminescence technique, the effective content of antioxidants and its reactivity towards peroxyl radicals have been measured in a model reaction of initiated oxidation of hydrocarbon for the whole extract and two of its chromatographic fractions separated by HPLC. The results indicate that the extract contains a combination of antioxidants with different radical scavenging activities.

[Antioxidant activity of flaxseed oil].

Effective concentration of antioxidants and its reactivity toward peroxil radicals (constant k7) have been measured by the chemiluminescence technique for flaxseed oil. Effective concentration of antioxidants is shown to depend on the technology of producing flaxseed oil; period of seed storage before use; and storing duration of flaxseed oil also. Minor component content of flaxseed oil, which may be the members of antioxidant pool, has been quantitatively estimated.

[Age changes in the level of pyridinoline cross-linking and ratio of soluble and insoluble collagen in human rib collagen].

The changes in the content of mature crosslinks with pyridinoline structure and soluble/insoluble collagen ratio in the costal cartilage tissue of human beings aged from 1 month to 57 years were found to be age-dependent. The effect of the pyridinoline crosslink content on the soluble/insoluble collagen ratio in human costal cartilage tissue may constitute no less than 67% of the total influence of the sum of all factors. The pronounced nonlinearity of the studied dependencies points to a possible involvement of a factor(s) other than the pyridinoline crosslink content.

[Comparative characteristics of oxyproline excretion in urine in children with post-burn hypertrophic and keloid scars].

Catabolism of collagen was studied in 25 children with keloid and hypertrophic scars by means of evaluation of total and polypeptide-bound hydroxyproline excreted with urine. Formation of postburn pathological scars led to an increase in excretion of collagen degradation products, the rate of the increase depended on area and age of scar. In hypertrophic cicatrization rate of collagen metabolites excretion correlated with the scar age reversely, demonstrating the tendency to decrease of the scar collagen metabolites with its ageing.

[The connection between the decomposition of mature collagen and the level of natural cytotoxicity in patients with focal scleroderma].

Increased rate of pyridinoline excretion with urine was detected in patients with focal sclerodermia: the ratio pyridinoline/creatinine was equal to 0.094 +/- 0.049 as compared with that of 0.

[Excretion of pyridinoline with urine in children with Ehlers-Danlos syndrome and Marfan's syndrome and the effect of beta blocker and complex vitamin therapy on it].

Excretion of pyridinoline and polypeptide-bound hydroxyproline with urine was studied in 27 children with hereditary impairment of connective tissue. At the same time, effects of beta-adrenoblocking agents and vitamin complex, prescribed during preoperation period before thoracoplasty in hereditary chest deformation, were investigated. Clinical efficiency of the treatment depended distinctly on the initial value of ratios pyridinoline/creatinine and polypeptide-bound hydroxyproline/creatinine.

[Physiological changes in pyridinoline excretion in urine depending on age and sex].

Natural physiological alterations in excretion of pyridoline (total hydroxypyridoline and lysyl pyridoline) with urine were studied in children and adults depending on age (1-86 years old) and sex. Age-dependent alterations of pyridoline excretion with urine had apparently four phases: an increase, a decrease, stabilization and secondary increase. Distinct dissimilarity between men and women in the rate of pyridoline excretion was found only in the age group of 16-21 years old.

[Early diagnosis of progression of funnel chest in children].

104 patients with progressive forms of funnel chest deformity of the II-III degree with Ehlers-Danlos-Marfan syndrome, Marfan-like phenotype, isolated funnel chest deformity and unclassified funnel chest deformity of the first degree were subjected to synromologic examination. There were detected 10 dysplastic signs, indicating funnel chest deformity progression: anti-Mongol shape of the eyes, arachnodactyly, high palate, Ehlers-Danlos syndrome, floor of the auricle's dysplasia, dolichostenomely, posture disturbance, mitral valve prolapse, umbilical hernia, wide filter. The results of biochemical examination of collagen metabolite--hydroxyproline++ (the first and the second hydroxyproline++ fraction ratio disturbance, decrease of the first hydroxyproline++ fraction percentage, disturbance of direct correlation between total hydroxyproline++ and percentage of the first hydroxyproline++ fraction)--corroborated the clinical data.

[The effect of collagen-normalizing therapy on the incidence and severity of anesthetic and postoperative complications in children with connective tissue syndromes].

A new method of collagen-normalizing therapy has been worked out to prevent specific anesthesia-induced, operative and postoperative complications and to treat some symptoms in children with Ehlers-Danlos and Marfan's syndromes and with non-classified Marfan-like malformations. The technique involves a combined use of beta-adrenoblocker in the age-matched doses and vitamins C/0.03 g/(kg.

[Effect of therapy with beta-adrenoblockers and vitamin complexes on indices of oxyproline excretion in various hereditary connective tissue diseases].

Excretion of hydroxyproline with urine was studied in 16 children (5-14 years old) with Marphan-Like syndrome and Marphan, Ehlers-Dunlos and Larson syndromes after therapy involving propranolol and a complex of vitamins (ascorbic acid, riboflavin and pyridoxine) and recommended on the basis of echocardiographic analyses. The therapeutic course appears to cause quantitative and qualitative correction of collagen and apparently of elastin fibrilles development. Depending on initial patterns of hydroxyproline excretion and the syndrome form the correction could be complete or partial, while positive effect of the treatment was stable or provisional.

[Relation between infections during the pregnancy and the rate of hydroxyproline excretion in children with congenital chest deformities].

In a group of women, which had children with hereditary isolated and syndromal chest deformations, acute respiratory diseases, tonsillitis or influenza occurred during pregnancy in 42.9 +/- 6.6% and 53.

[Possible role of natural cytotoxicity in impairment of collagen catabolism in focal scleroderma].

Relationship between the state of natural cytotoxicity and collagen catabolism were studied in patients with focal sclerodermia. In the impairment activity of natural killer cells (NK cells) was markedly altered depending on such clinical parameters as duration, severity, activity and distribution of the disease as well as it was related to definite transformations in catabolism of collagen proteins. Low rate of collagen production and intensive collagenolytic processes correlated with the high level of hydrolytic activity of NK cells at the early steps of systemic morphea guttata accompanied by highly progressive course of the disease.

[Urinary excretion of hydroxyproline in funnel chest deformity].

Excretion of hydroxyproline with urine was studied in 16 children with localized form of funnel chest deformation simultaneously with Marfan and Ehlers-Danlos syndromes, in 9 children with the localized form of deformation within 6-8 months after thorax surgical plastic operation as well as in 3 children with Ehlers-Danlos syndrome but without funnel chest deformation. Funnel chest deformation of the II-III degree, independently of its form, was accompanied by a decrease of total hydroxyproline in urine as compared with healthy children of the similar age. The hydroxyproline excretion was normalized after thoracoplastic operation in the children with localized form of the chest deformation.

[Age-related characteristics of urinary excretion of oxyproline].

Age-dependent alterations in excretion of hydroxyproline were studied in 62 healthy persons (3-78 years old) by means of analysis of the morning portion of urine after gel filtration on Sephadex G-10. With ageing the following patterns were decreased: content of total hydroxyproline (r = -0.78; P less than 0.

[Tissue enzymatic study in the perfusate of cadaveric kidneys for assessing their functional state].

A correlation was found between the level of the tissue enzyme activity in cadaveric kidneys perfusates and the degree of their functional adequacy in the early posttransplantation period. According to the data obtained the level of the activity of leucin aminopeptidase and gamma-glutamyltranspeptidase in the perfusates may be of importance for prognostication of a delayed function of the kidneys after transplantation. An elevated level of activity, in addition to leucin adminopeptidase and gamma-glutamyltranspeptidase, lactatedehydrogenase, aspartataminotransferase, muramidase, acid and alkali phosphatases suggests the transplanted kidney to be not functioning.

[Homogenate factor of minks with Aleutian disease].

Antibodies to the factor present in the homogenate of the organs of minks experimentally infected with aleutian disease (AD) were revealed in the sera of minks with AD and also in the sera of humans suffering from systemic lupus erythematosus, hepatitis and cirrhosis, scleroderma and dermatomyositis. Sera of sick minks and humans failed to interact with control homogenates of the organs of healthy minks. In turn, sera of practically healthy minks and humans did not react with the AD-homogenate.