Clinical and immunological characteristics of sarcomas patients with clonogenic tumors.

Tumorigenesis is related to the generation of heterogeneous tumor cell population, which is the result of genetic and epigenetic alterations followed by clonal selections and subsequent expansion. In basic studies genetic, histological and morphological diversity of different clones within a patient's neoplasm and specifics of their interrelation with patient's immune system are investigated mostly on the models of tumors of epithelial origin. Mesenchymal tumors such as soft tissue and bone-derived sarcomas (STBS) have been poorly studied in this regard.

[CHRONOBIOLOGICAL ACTIVITY OF AQUEOUS EXTRACTS OF THE ABOVE-GROUND PART OF AGRIMONIA PILOSA L. IN RATS.].

It is established that lithium is accumulated in the above-ground part of agrimony (Agrimoniapilosa L.), the aqueous extract of which exhibits lithium-specific chronobiological activity in rats. The chronobiological study was carried out during the winter solstice on 40 adult male rats divided into three groups: control (intact animals treated with purified water and the extract purified from lithium) and two test groups.

[Reactivity of the central nervous system influences the sensitivity of secretory cells of the stomach to carbacholine and glycyl-proline (Gly-Pro)].

In experiments using cluster analysis of the behavioral activity of rats in the "open field" test, the animals were divided into three groups with different types of behavior and response to the first presentation of the test: (i) active-search (all the studied reactions are expressed), (ii) intermediate, and (iii) passive-defensive (lowest level of activity). Differences in the indices of carbacholine-stimulated gastric secretion in rats of different groups were manifested by greater secretion of the stomach and lower digestive capacity of gastric juice observed in rats with active-search and intermediate types of behavior in the "open field" test in contrast to rats with passive-defensive type. Combined administration of cholinomimetic and glycyl-proline (Gly-Pro) lead to decreased proteolytic activity of gastric juice in rats with active-search behavior in the "open field" test, decreased volume of gastric secretion in rats with an intermediate type of behavior, and increased volume of juice and its decreased digestive ability in rats with passive-defensive behavior.

[THE INFLUENCE OF LEU-ENKEPHALIN AND MEDICAL PLANTS ON MOTOR ACTIVITY OF STOMACH IN DOGS].

The aim of the real research was a study influence of leu-enkephalin and extracts from the leaves of goose-grass large, of burdock of felted and root of chicory on the motor function of stomach for dogs. The study was carried out on 6 outbred dogs-males, by mass 14-17 kg, with the fistula of stomach by Basov. A leu-enkephalin ("Vector", Russia) was entered intravenously in a dose 7 mcg/kg.

[Treatment of surgical stress response by neuroleptics, leu-enkephalin, glycyl-proline dipeptide, and electroanesthesia in experimental animals].

We have studied the effects of chlorpromazine, acepromazine, droperidol, and transcranial electroanesthesia upon evacuation function of the stomach in piglets and the effects of leu-enkephalin and glycyl-proline upon secretory activity of the stomach in dogs and rats during surgical stress to optimize anesthetic dosage. All pharmaceutical and nonpharmaceutical methods of anesthesia used in the experiments were implemented by actiovating stress-limiting systems in post-operational period. Leu-enkephalin, apart from stimulating the stress-limiting factors of neurohumoral systems of the organism, shifted the vector ratio of aggressive and protective factors of gastric mucosa toward strengthening of the latter.

Optimization of parameters of physiological systems in animals under conditions of experimental stress exposure.

Physiological and biomedical experiments were performed. We estimated the parameters of tree main levels of physiological systems as a response of an organism to stress. Mechanisms of physiological defense of an organism against stress should be considered systemically.

[Leu-enkephalin modulates gastric secretion activated by various stimulants].

The results of chronic experiments on dogs showed that leu-enkephalin in doses 1, 7, and 9 microg/kg produced different effects on the gastric secretion and excretion on the background of various stimulants. The maximum protective effect of opiate was produced in a dose of 7 microg/kg. The peptide effect depended on the gastric secretion stimulator used: on the background of carbachol and pentagastrine, the activity of acidic and peptic factors was decreased and the protective factors of gastric juice were increased.