Feline mammary carcinomas as a model for human breast cancer. II. Comparison of in vivo and in vitro adriamycin sensitivity.

Feline mammary carcinomas were tested for their in vitro and in vivo sensitivity to Adriamycin. In vitro, twenty randomly chosen tumor islands were studied to test the effect of concentrations of the drug ranging from 0.25-2.

Representativity of human mammary tumor cell cultures: DNA-cytophotometry as a method for checking tumour cell characteristics.

Short term cultures (3-6 days) of 40 primary human mammary carcinomas were prepared and compared with the original tumours from which they were derived. As a criterion the nuclear DNA Frequency Distribution Pattern (FDP), cytophotometrically measured, was used. Comparisons were made between the FDPs of smears of freshly-cut tumour surfaces and their cultures.

Feline mammary carcinomas as a model for human breast cancer. I. Sensitivity of mammary tumor cells in culture to cytostatic drugs. A preliminary investigation of a predictive test.

Feline mammary carcinomas were found to maintain well in short-term cultures. Principally the same types of nuclear DNA frequency distribution histograms were recognized in feline mammary carcinomas as in human mammary carcinomas. However, the more abnormal histograms are less frequent in feline than in human mammary carcinomas.

Morphogenesis in mammary gland cell cultures. Occurrence of tubular structures in combined cultures of fibroblastic and normal epithelial cells.

In cultures with a dense confluent layer of fibroblasts mixed with a small population of normal mouse mammary epithelial cells, the latter form tubular structures that can be stimulated to secretion by hormones.

Delphinidin- and cyanidin-chlorides, promotors of cell aggregation in vitro.

Delphinidin-chloride and Cyanidinchloride cause cells in cell culture to form aggregates instead of the usual monolayers, without impairing viability or proliferation. The process is reversible.

[Tissue culture investigations on the distinction between hereditary and sporadic retinoblastoma (author's transl)].

The growth pattern in tissue culture of cells derived from retinoblastoma is different for all cases with known heredity and most of the cases with unknown heredity. It is concluded that those of the latter, that show a growth pattern that is identical with that of the hereditary cases, represent the hereditary cases in the group with unknown heredity.

Model approaches for evaluation of cell coupling in monolayers.

The electrical coupling of epithelial cells of the intestine of chick embryo cultured in monolayers was studied. This coupling can be evaluated by regarding the monolayer as a honeycomb structure of cells and the cells as equipotential spaces, as long as the cells are small (diameter <25μm). With help of this discrete model it was found that for the non-junctional membrane the membrane resistance isϱ m =250-2,000 Ω cm(2), and for the junctional membraneϱ m =5-50 Ω cm(2).