Biological activity of amino acids in organotypic tissue cultures.

We studied the effect of 20 standard L-amino acids on proliferation of the nervous, cardiovascular, urogenital, digestive, and immune system tissues from young and old animals in organotypic cultures. The effect of amino acids on tissue culture proliferation depended on their origin and animal age.

Peptidegic regulation of differentiation of embryonic retinal cells.

We studied Molecular mechanisms of the retinoprotective effect of short peptides AEDG and KE. The peptides stimulate differentiation of neurons and retinal pigment epithelium cells and therefore can be considered as potential retinoprotective preparations for the treatment of age-related degenerative changes in the retina.

[Effect of peptide bioregulators and cytokines on life span and age-related changes of hemostasis].

The review considers literature data and own results of research of cytokines functions and their effects on hemostatic system and life span. The data of age-related changes in the hemostatic system is presented in this article. A big part of the review is devoted to the action of regulatory peptides (RP) on various body systems.

Short cell-penetrating peptides: a model of interactions with gene promoter sites.

Analysis of the main parameters of molecular mechanics (number of hydrogen bonds, hydrophobic and electrostatic interactions, DNA-peptide complex minimization energy) provided the data to validate the previously proposed qualitative models of peptide-DNA interactions and to evaluate their quantitative characteristics. Based on these estimations, a three-dimensional model of Lys-Glu and Ala-Glu-Asp-Gly peptide interactions with DNA sites (GCAG and ATTTC) located in the promoter zones of genes encoding CD5, IL-2, MMP2, and Tram1 signal molecules.

A method of creation a cell monolayer based on organotypic culture for screening of physiologically active substances.

We developed a method of culturing and phenotyping of a monolayer of cells of the retinal tissue, thymus and spleen on the basis of organotypic culture. All characteristic types of neurons and fibroblasts were found in their microenvironment in the retinal cell monolayer. Lymphocytes, macrophages, and fibroblasts were verified in the monolayer of thymus and spleen cells.