Promising New Salt of Oseltamivir.

A synthetic method for the promising new drug oseltamivir ethoxysuccinate is described in detail. Various conditions for obtaining the target substance are considered. Its complete physicochemical characteristics are given.

Biological characteristics of influenza A(H1N1)pdm09 virus circulating in West Siberia during pandemic and post-pandemic periods.

We studied biological characteristics of influenza A(H1N1)pdm09 virus circulating in Siberia during the 2009 pandemic and the post-pandemic period of 2011. BALB/c mice were chosen as the experimental model. Virus titers in the lungs were evaluated on days 1, 3, 6 and blood serum titers on day 15 after infection with different strains.

[Peculiarities of pre- and postnatal kidney development in vasopressin-deficient brattleboro rats].

The objective of this study was to examine pre- and postnatal development of the kidney in vasopressin-deficient Brattleboro rats in comparison as compared to that in Wistar rats. Histological, histochemical and morphometric methods at light microscopic level were used. The study included 50 fetuses at gestational days 16 and 18, and 46 rat pups at postnatal days 5, 10, 20, and 30.

Biomatrix on the basis of polypropylene mesh and fetal fibroblasts.

A technology for obtaining a biomatrix on the basis of polypropylene mesh, used in clinical surgery, and cultured fetal fibroblasts is proposed. The method was developed on experimental animal cells. It includes culturing of rat fetal fibroblasts on fibers of a polypropylene mesh, treated with wheat germ lectin.

Effects of central opiate and serotoninergic structures on heart rhythm during acute myocardial ischemia.

Electrostimulation of the central gray matter in the sylvian aqueduct and nucleus raphe magnus produced an antiarrhythmic effect during acute myocardial ischemia. Stimulation and blockade of opiate receptors in the central amygdaloid nucleus and lateral hypothalamus with dalargin and naloxone induced the same effect. Destruction of the central gray matter in the sylvian aqueduct and nucleus raphe magnus decreased electrical stability of ischemic myocardium.

[Cytokine production during the development and correction of an immunodeficiency in experimental tuberculosis].

The authors investigated spontaneous and induced secretion of cytokins at different stages of generalized tuberculosis. In the development of infection there were inhibited IL-2 synthesis in response to ConA, emerging activity of PNO-alpha in response to the inductors in blood serum and culture of peritoneal macrophages, enhanced secretion of IL-6. Complete immunodeficiency was associated with cessation of IL-2 synthesis by splenocytes, elevated production of IL-6 by peritoneal macrophages, low concentrations of PNO-alpha in the serum and peritoneal macrophage cultures.

[Coxiella burnetii antigens--inducers of tumor necrosis factor and interleukin-1].

The cultivation of mouse peritoneal macrophages in the presence of antigenic preparations obtained from C.burnetii was accompanied by the appearance of phagocytes and considerable amounts of tumor necrosis factor and interleukin 1 in the culture medium. The production of cytokines depended on the doses of preparations used as inducers.

[The induction of tumor necrosis factor and interleukin-1 under exposure to the glycopolymers isolated from Pseudomonas solanacearum and Clavibacter michiganense].

Lipopolysaccharide of Pseudomonas solanacearum and acid polysaccharides of Clavibacter michiganense are effective inductors of formation of the factor of tumour necrosis (TNF) and interleukin-1 (IL-1) by peritoneal macrophages of mice and their activity exceeds that of lipopolysaccharide Escherichia coli 055:B5 ("Sigma"). O-specific polysaccharide and lipid A are responsible for the capacity of liposaccharide molecule of P. solanacearum to induce TNF and IL-1.

[A photometric method for assessing the proliferation of cell cultures].

A possibility to evaluate the quantity of cultured cells was examined using the optical density of the stain eluate after absorbtion by cells in the course of staining of the cellular monolayer. The investigation of the growth dynamics of primary trypsinized cultures of embryonic fibroblasts using the proposed criteria has shown its relationship with the quantity of cells in culture. It is proposed that this method may be used for quantitative characteristics of cell cultures.

[Mechanism of the anti-tumoral effect of the blastolysin fraction isolated from Lactobacillus bulgaricus].

A blastolysin fraction, isolated from lysosyme lysates of Lactobacillus Bulgaricus strain 51 by gel chromatography, was shown to markedly inhibit the growth of sarcoma S-180. A single administration of the agent was followed by an insignificant decrease in tumor growth rate, while repeated treatment induced a complete regression of tumor in 15% of animals and resulted in a prolonged suppression of tumor growth in the others. Cells isolated from metastases which developed after the withdrawal of the drug retained susceptibility to fraction 2 of blastolysin.