Preoperative blood markers for prediction of recurrence-free survival after surgical treatment of patients with stage III lung adenocarcinoma.

The possibility of the preoperative level of 42 indicators characterizing the cellular composition and metabolism in blood of patients with stage III lung adenocarcinoma (AC) to predict their relapse-free survival was studied. Blood samples of 451 patients with newly diagnosed AK stage III after their surgical treatment (resection volume - R0) have been investigated. The duration of the relapse-free period (period of observation - 1 year), cellular composition of the blood, concentration of C-RP, albumin, Cyfra 21-1 antigens, SCC, TPA, chemokines CXCL5, CXCL8, pyruvate kinase TuM2 PK isoenzyme, HIF-1α and hyaluronic acid in blood serum so as the proportion of blood cells with CXCR1 and CXCR2, CD44V6 receptors in blood serum were measured.

On the issue of diagnostic value of determining the level of receptors and their ligands in blood in non-small cell lung cancer.

Non-small cell lung cancer (NSCLC) occupies the first place in the structure of mortality due to oncological diseases. Late diagnosis worsens the effectiveness of its treatment. There are no informative biomarkers that allow us to judge the prevalence of the tumor process, especially in the early stages of NSCLC.

[Diagnostic efficiency of determining CXCR1, CXCR2 and hyaluronic acid blood level in non-small cell lung cancer patients].

In the structure of lung cancer incidence most cases belong to non-small cell lung cancer (NSCLC) which is subdivided into two histological subtypes: adenocarcinoma (AC) and squamous cell carcinoma (SCC). A five-year survival rate of patients with stage I NSCLC is two times higher than in patients with stage II and more than five times higher than in stages III-IV. Currently, there are no informative blood biomarkers to diagnose early stages of NSCLC.

[Glutathione-dependent regulation of platelet aggregation with neutrophils and tumor cells].

It is shown that in the presence of reduced glutathione at low concentrations (1-5 microM) the extent of platelet aggregation with neutrophils increases and the lag period of platelet aggregation induced by tumor cells decreases. At the same time in the presence of reduced glutathione at high concentration (3 mM) the extent of platelet aggregation with neutrophils decreases, and the lag period of platelet aggregation induced by tumor cells increases. It is established that glutathione-dependent regulation of the intercellular contact formation between platelets and neutrophils depends on the ratio of glutathione oxidized and reduced forms: at fixed total glutathione concentration of 5 microM, increase of glutathione redox potential from -175 mV to 0 mV led to reduction in platelet aggregation with neutrophils.

[Evaluation of the double bonds of lipid fatty acids by ozonization in cancer patients].

The diagnostic value of total unsaturation of plasma lipids was studied by ozonization in 950 patients with malignant neoplasms. An examination was made on their primary admission. The control group included 470 patients with chronic benign diseases of the studied sites and 205 clinically healthy persons.

Oxidative metabolism of rat blood in the course of alveolar hepatic carcinoma PC-1 growth.

Aim: To evaluate oxidative metabolism of rat blood in the course of alveolar hepatic cancer growth in vivo.

Methods: The oxidation imbalance was assessed by the rise in the values of the integral index of oxidation stress. The structural and functional state of erythrocyte membranes was investigated by spin electron paramagnetic resonance spectroscopy.

[Antimicrobial activity of piperacillin in vitro].

There was an increase in the resistance of clinical isolates to piperacillin as compared to that in 1987-1988. It was shown on the kinetic models that there was difference in the effect of the inhibitory and subinhibitory concentrations of the drug on microorganisms in various growth phases. No post-antibiotic action against Staphylococcus aureus and Pseudomonas aeruginosa and a slightly pronounced dose-dependent effect with respect to Escherichia coli were noted.

[Antimicrobial activity of ceftazidime and its pharmacokinetics in patients with renal failure].

High antimicrobial activity of ceftazidime against clinical gram-positive (except Streptococcus viridans and S. faecalis) and gram-negative strains of microorganisms was shown. It was found that the drug dosing regimens required correction in cases with impaired excretion function of the kidneys.

[The prevention of the hypercoagulation syndrome during the combined treatment of lung cancer patients using artificial hyperglycemia].

Status of hemostasis system and occurrence of postoperative thromboembolic complications were assessed in 246 lung cancer patients in whom combined treatment included artificial hyperglycemia. The latter condition contributed to higher blood coagulability resulting in lung artery thromboembolism in some cases. Administration of a combination of heparin, curantil and nicotinic acid was followed by a decrease in blood coagulability, increase in anticoagulation function, decrease in platelet aggregability, activation of fibrinolysis and regression of biochemical signs of DIC syndrome thereby assuring a drop in the occurrence of thromboembolic complications.

[Radionuclide studies on the hormonal manifestations of heat stress in cancer patients under controlled total hyperthermia].

The results of the investigations of ACTH, HGH, hydrocortisone, glucagon, C-peptide, insulin in 180 patients with advanced and metastatic melanomas, soft tissue sarcomas, lung cancers and renal cell carcinomas testify to the development of the syndrome of endocrine hyperfunction in patients under whole-body guided hyperthermia as well as of functional pancreas insufficiency. The data presented form a biochemical basis for working out measures to optimally carry out whole-body hyperthermia treatment, aimed at increasing the range of indications for its use in clinical oncology.

[The effect of whole-body controlled hyperthermia on the energy resources of cancer patients].

The results of examination of 120 patients with advanced malignant melanoma, soft tissue sarcomas and lung and renal cancer suggest disturbance in mechanisms of energy supply during whole-body guided hyperthermia. Cell glucose uptake was inhibited in hyperglycemic patients. High energy loss in due to utilization of such alternative substrates as lipids and proteins which, in turn, potentiates peroxidation of lipids toxic to cell membranes.

[Infusion therapy in sessions of general artificial hyperthermia using large doses of insulin in cancer patients].

A scheme of infusion correction of water-electrolyte shifts occurring during general artificial hyperthermia sessions (rectal t degrees-40-42 degrees C) employing large insulin doses (100-1120 U) in cancer patients has been suggested. The infusion therapy according to the technique described was used during 103 general artificial hyperthermia sessions in 58 patients. This made it possible to maintain physiologically normal blood plasma levels of glucose, Na+, K+, calcium, inorganic phosphates, normal acid-base balance, adequate hydration and circulating blood volume.

[Effects of induced hyperglycemia on the activity of pancreatic and gastrointestinal secretion of hormones in oncologic patients].

Artificial hyperglycemia (AHG) used in the cancer treatment considerably increases the insulin and S-peptide content in blood, decreases the glucagon concentration and tissue glucose tolerance. The changes are reversible, have a functional character, that indicates the absence of a disbalance in hormonal mechanisms of carbohydrate metabolism control as well as marked disorders of the pancreas function. In order to achieve a wider range of indications for using AHG in complex schemes of the cancer treatment, it is necessary to take measures on optimization of the carbohydrate metabolism state which should be controlled by the suggested complex of laboratory tests.