Chemical complexity of protein determines optimal expression host; A comparative study using Erythropoietin, Streptokinase and Tumor Necrosis Factor Receptor.

High throughput expression of proteins is often hampered by the failure of certain proteins to express in the particular host strain used for the study. The identification of a host strain compatible for a wide variety of proteins is desirable. In this study, the recombinant expression of therapeutic proteins Erythropoietin (EPO), Streptokinase (SK) and Tumor Necrosis Factor Receptor Extra cellular domain (TNFR ED) that vary widely in their chemical nature was studied in four different strains of namely BL21 (DE3), BL21 (DE3) pLys S, BL21 (DE3) Rosetta pLys S and GJ1158.