Role of Trained Immunity in Heath and Disease.

Purpose Of Review: This review aims to explore the role of immune memory and trained immunity, focusing on how innate immune cells like monocytes, macrophages, and natural killer cells undergo long-term epigenetic and metabolic rewiring. Specifically, it examines the mechanisms by which trained immunity, often triggered by infection or vaccination, could impact cardiac processes and contribute to both protective and pathological responses within the cardiovascular system.

Recent Findings: Recent research demonstrates that vaccination and infection not only activate immune responses in circulating monocytes and tissue macrophages but also affect immune progenitor cells within the bone marrow environment, conferring lasting protection against heterologous infections.

Pharmacodynamics of NOSO-502 studied in vitro and in vivo: determination of the dominant pharmacodynamic index driver.

Background: NOSO-5O2 is the first clinical candidate of a new antimicrobial class-the odilorhabdins. The pharmacodynamics of NOSO-502 were studied in vitro and in vivo to establish the pharmacodynamic index (PDI) driver.

Methods: A dilutional pharmacokinetic system was used for in vitro experiments.

French marigold () flavonoid extract-based priming ameliorates initial drought stress on var , cultivar Satabdi (IET4786): a sustainable approach to avoid initial drought stress.

Drought stress remains a serious concern in L. var , cultivar Satabdi (IET4786) production, particularly during the earliest growth phases, ultimately affecting yield due to the recent trend of delayed rain arrival in West Bengal, India. This study aimed to develop a cost-effective strategy to improve the drought tolerance capacity of rice seedlings by priming the seeds with flavonoid-enriched extract (FEE) of French marigold () petals to withstand the initial drought milieu.

An Entropy-Based Approach to Model Selection with Application to Single-Cell Time-Stamped Snapshot Data.

Recent single-cell experiments that measure copy numbers of over 40 proteins in individual cells at different time points [time-stamped snapshot (TSS) data] exhibit cell-to-cell variability. Because the same cells cannot be tracked over time, TSS data provide key information about the time-evolution of protein abundances that could yield mechanisms that underlie signaling kinetics. We recently developed a generalized method of moments (GMM) based approach that estimates parameters of mechanistic models using TSS data.

A framework integrating multiscale in-silico modeling and experimental data predicts CD33CAR-NK cytotoxicity across target cell types.

Uncovering mechanisms and predicting tumor cell responses to CAR-NK cytotoxicity is essential for improving therapeutic efficacy. Currently, the complexity of these effector-target interactions and the donor-to-donor variations in NK cell receptor (NKR) repertoire require functional assays to be performed experimentally for each manufactured CAR-NK cell product and target combination. Here, we developed a computational mechanistic multiscale model which considers heterogenous expression of CARs, NKRs, adhesion receptors and their cognate ligands, signal transduction, and NK cell-target cell population kinetics.

A Descriptive Study of Patients Presenting With Topical Steroid Damaged/Dependent Face (TSDF) to a Tertiary Care Center in Northern India.

Introduction Topical steroid damaged/dependent face (TSDF) is defined as the semi-permanent or permanent damage to the skin of the face precipitated by the irrational, indiscriminate, or prolonged use of topical corticosteroids (TCs), resulting in various cutaneous signs and symptoms and psychological dependence on the drug. The objective was to determine the clinical spectrum of TSDF. Methods This was an observational cross-sectional study conducted between May 2021 and April 2022, comprising 100 consecutive patients of TC-induced facial dermatoses who visited the skin and venereal disease OPD of a tertiary care hospital in northern India.

Volumetric Comparison of the Temporomandibular Joint Space in Skeletal Class I and II Patients Using Cone-Beam Computed Tomography: A Cross-Sectional Study.

Introduction: The temporomandibular joint (TMJ) is one of the most intricate anatomical entities within the human body and is clinically relevant in the field of dentistry. Therefore, the present study aimed to conduct a three-dimensional (3D) volumetric comparison of the TMJ space in skeletal Class I and II patients using cone-beam computed tomography (CBCT).

Materials And Methods: This cross-sectional, retrospective study was conducted on 40 CBCT records divided into skeletal Class I and skeletal Class II patients.

Molecular Characterization and Potential Inhibitors Prediction of Protein Arginine Methyltransferase-2 in Carcinoma: An Insight from Molecular Docking, ADMET Profiling and Molecular Dynamics Simulation Studies.

Objectives: To predict and characterize the three-dimensional (3D) structure of protein arginine methyltransferase 2 (PRMT2) using homology modeling, besides, the identification of potent inhibitors for enhanced comprehension of the biological function of this protein arginine methyltransferase (PRMT) family protein in carcinogenesis.

Materials And Methods: An method was employed to predict and characterize the three-dimensional structure. The bulk of PRMTs in the PDB shares just a structurally conserved catalytic core domain.

Snow White's tale in nephrology: the emerging threat of skin-whitening creams on kidney health.

The timeless tale of Snow White, with its emphasis on fair skin as a beauty ideal, mirrors a contemporary issue in nephrology: the harmful impact of skin-whitening creams on kidney health. Fairness creams have deeply embedded themselves in global society, driven by a pervasive obsession with lighter skin tones as a symbol of beauty. This widespread use reflects deeply rooted cultural beliefs and social norms, despite the significant health risks associated with these products.

Evaluation of Point-of-care Ultrasound of Airway to Predict Difficult Laryngoscopy and Intubation in Intensive Care Unit Patients.

Background: To evaluate the role of ultrasound (US) in the assessment of the airway and to determine whether US has the potential to serve as effective, noninvasive and less time-consuming method for the diagnosis of difficult intubation in ICU patients.

Patients And Methods: This cross-sectional study was carried in 152 critically ill patients who underwent intubation in the ICU from December 2022 to April 2024. Prior to intubation thyromental height (TMH) and hyomental distance ratio (HMD-R) was measured using a scale and distance from skin to hyoid bone (SHB) and distance from skin to thyrohyoid membrane (STM) was measured using a US.

Threats of zoonotic pathogens in food chain: Current status and gaps in India.

Background: Public health is seriously threatened by transmission of zoonotic infection through the food chain. Factors like increasing population, deforestation, high demand for animal protein, and trade of sub-clinically infected animals are the main causes of the spread of infections from asymptomatic animals to humans. Despite several national programs like (The Clean India Mission) prevention of open defecation and water, sanitation, and hygiene (WASH), the incidence of diarrhoeal diseases remains high in India.

Fluorescence tunable carbon dots for nuclear dynamics and gastrointestinal imaging in live zebrafish and their toxicity evaluation by cardio-craniofacial disfunction assessment.

Sub-cellular organelle anomalies are frequently observed in diseases such as cancer. Early and precise diagnosis of these alterations can be crucial for patient outcomes. However, current diagnostic tools using conventional organic dyes or metal quantum dots face limitations, including poor biocompatibility, stringent storage conditions, limited solubility in aqueous media, and slow staining speeds.

Inguinal hernia mesh infections: Chronic challenges, atypical pathogens and lessons in sterilisation.

Chronic mesh infections after inguinal hernia repair present significant clinical challenges due to biofilm-mediated resistance, involvement of multidrug-resistant and atypical pathogens, and gaps in preventive strategies. Our case series of four patients highlights critical research gaps, including the overlooked role of atypical pathogens such as , diagnostic challenges in detecting slow-growing or resistant organisms and perioperative sterilisation lapses, especially inconsistent Glutaraldehyde use during late-day operations. Many patients suffered with persistent sinuses and recurrent hernias months after surgery.

Side-Gated Iontronic Memtransistor: A Fast and Energy-Efficient Neuromorphic Building Block.

Iontronic memtransistors have emerged as technologically superior to conventional memristors for neuromorphic applications due to their low operating voltage, additional gate control, and enhanced energy efficiency. In this study, a side-gated iontronic organic memtransistor (SG-IOMT) device is explored as a potential energy-efficient hardware building block for fast neuromorphic computing. Its operational flexibility, which encompasses the complex integration of redox activities, ion dynamics, and polaron generation, makes this device intriguing for simultaneous information storage and processing, as it effectively overcomes the von Neumann bottleneck of conventional computing.

Long-term Prospective Comparative Analysis of Ototoxic and Survival Outcomes of Sequential Boost and Simultaneous Integrated Boost of Volumetric Modulated Arc Therapy for Head-Neck Carcinomas.

Objective: To compare the ototoxicity and survival in head and neck carcinoma patients treated with sequential (SEQ) and simultaneous integrated boost (SIB) of volumetric modulated arc therapy (VMAT).

Methods: This long-term prospective study enrolled patients with histologically confirmed head and neck carcinoma, all receiving VMAT treatment. Audiological assessments were done using various tests at baseline, two weeks, treatment completion, six months, and 12 months.

Genotypic and phenotypic diversity of Mycobacterium tuberculosis strains from eastern India.

Whole genome sequencing has been used to investigate the genomic diversity of M. tuberculosis in the northern and southern states of India, but information about the eastern part of the country is still limited. Through a sequencing-based strategy, this study seeks to comprehend the diversity and drug resistance pattern in the eastern region.

Outcomes of Extremely Preterm Infants Exposed to Prolonged Prelabor Rupture of Membranes before 24 Weeks of Gestation.

Background: Preterm prelabor rupture of membranes (PPROM) before or around the limit of fetal viability is associated with serious maternal and neonatal complications including chorioamnionitis, extremely preterm birth, and pulmonary hypoplasia.

Objectives: To describe contemporary outcomes of extremely preterm infants born after prolonged periviable PPROM, and to identify perinatal factors associated with survival and survival without severe neurodevelopmental impairment (NDI).

Study Design: Among actively treated infants born alive at <27 weeks' gestational age (GA) in centers of the Eunice Kennedy Shriver National Institute of Child Health and Human Development Neonatal Research Network from 2012 to 2018, the outcomes of survival and survival without severe NDI at 22-26 months' corrected age were compared between infants exposed to prolonged (≥120 hours) periviable (<24 weeks' GA) PPROM and unexposed infants born after rupture of membranes ≤18 hours before delivery or at delivery, adjusting for birth GA, sex, multiple gestation, antenatal steroids, small for gestational age (SGA), insurance, and center.

Deep Learning for Classification of Inflammatory Bowel Disease Activity in Whole Slide Images of Colonic Histopathology.

Grading activity of inflammatory bowel disease (IBD) using standardized histopathological scoring systems remains challenging due to limited availability of pathologists with IBD expertise and inter-observer variability. In this study, a deep learning model was developed to classify activity grades in hematoxylin and eosin-stained whole slide images (WSIs) from patients with IBD, offering a robust approach for general pathologists. This study utilized 2,077 WSIs from 636 patients who visited Dartmouth-Hitchcock Medical Center in 2018 and 2019, scanned at 40× magnification (0.

Moxifloxacin-loaded PVA-chitosan composite films as potential ocular drug delivery systems: A comprehensive characterization and efficacy assessment.

To overcome the barriers often met by traditional ophthalmic formulations, polymeric films can be utilized as an alternative to enhance drug retention duration while managing medication release. In the current investigation, polymeric films made of poly (vinyl) alcohol (PVA) and chitosan (CS) loaded with Moxifloxacin Hydrochloride (M-HCl) and plasticized with Glutaraldehyde were formulated as potential ophthalmic delivery for the treatment of conjunctivitis. The thickness, surface pH, opacity, folding endurance, and % hemolysis were measured, followed by the transparency, microscopy, electrical conductivity, mechanical strength, swelling index, and invitro drug release studies.

An efficient deep learning system for automatic detection of Acute Lymphoblastic Leukemia.

Early and highly accurate detection of rapidly damaging deadly disease like Acute Lymphoblastic Leukemia (ALL) is essential for providing appropriate treatment to save valuable lives. Recent development in deep learning, particularly transfer learning, is gaining a preferred trend of research in medical image processing because of their admirable performance, even with small datasets. It inspires us to develop a novel deep learning-based leukemia detection system in which an efficient and lightweight MobileNetV2 is used in conjunction with ShuffleNet to boost discrimination ability and enhance the receptive field via convolution layer succession.

Simultaneous detection of dengue virus serotypes in a dual-serotype-detection nucleic acid based lateral flow assay.

Dengue virus (DENV) is an important arthropod-borne viral disease, with four antigenically and genetically diverse serotypes (DENV-1, DENV-2, DENV-3, and DENV-4). Timely and accurate diagnosis of dengue virus serotypes is crucial for the management of outbreaks. This study focussed on the development of a RT-PCR based lateral flow strip assay to detect DENV serotypes in a dual detection manner without using gel electrophoresis.

Microsurgical preservation of lenticulostriate artery perforators in insular glioma: the two point antegrade skeletonization technique.

Background: Reaching parenchymal segments of the lateral lenticulostriate artery (LSA) perforators, which represent the medial resection limit in insular gliomas (IG), remains a challenge. The currently described methods are indirect and sometimes, imprecise.

Methods: We report an antegrade direct skeletonization technique to identify these tiny arteries at the medial end of IGs with an illustrative case of grade 2 astrocytoma.

Alkoxy-Directed Dienamine Catalysis in [4 + 2]-Cycloaddition: Enantioselective Synthesis of Benzo-[3]-ladderanol.

Despite tremendous progress of dienamine catalysis along with its application in enantioselective synthesis over nearly two decades, certain limitations, especially with respect to the regioselectivity in the dienamine generation step, continue to persist. To overcome these shortcomings of classical dienamine catalysis, we now introduce the concept of alkoxy-directed dienamine catalysis and apply it to the enantioselective arene construction by desymmetrizing -enediones through [4 + 2]-cycloaddition. Catalyzed by a diphenylprolinol silyl ether, this reaction utilizes γ-alkoxy α,β-unsaturated aldehydes as the substrate and proceeds in a highly regioselective fashion through the intermediacy of δ-alkoxy dienamine.

Examining Health and Social Support Among University Housekeepers: A Mixed-Methods Exploratory Study.

This pilot, exploratory project examined the relationship among the health, work, and social support of university housekeepers. The first objective was to examine the influence of social support on work-related outcomes among university housekeepers. The secondary objective was to examine the influence of social support on physical and psychological health among housekeepers.