Publications by authors named "Priti Rathod"
is the leading cause of death from bacterial infection. Improved rapid diagnosis and antimicrobial resistance determination, such as by whole-genome sequencing, are required. Our aim was to develop a simple, low-cost method of preparing DNA for sequencing direct from -positive clinical samples (without culture).
View Article and Find Full Text PDFM. tuberculosis grows slowly and is challenging to work with experimentally compared with many other bacteria. Although microtitre plates have the potential to enable high-throughput phenotypic testing of M.
View Article and Find Full Text PDFThe detection of laboratory cross-contamination and mixed tuberculosis infections is an important goal of clinical mycobacteriology laboratories. The objective of this study was to develop a method to detect mixtures of different lineages in laboratories performing mycobacterial next-generation sequencing (NGS). The setting was the Public Health England National Mycobacteriology Laboratory Birmingham, which performs Illumina sequencing on DNA extracted from positive mycobacterial growth indicator tubes.
View Article and Find Full Text PDFBackground: Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR) typing is widely used in high-income countries to determine Mycobacterium tuberculosis relatedness. Whole-genome sequencing (WGS) is known to deliver greater specificity, but no quantitative prospective comparison has yet been undertaken.
Methods: We studied isolates from the English Midlands, sampled consecutively between 1 January 2012 and 31 December 2015.
Article Synopsis
- Whole-genome sequencing (WGS) is gaining traction as a reliable method for identifying mycobacterial species and testing for drug resistance compared to standard laboratory methods.
- In a study involving 2,039 mycobacterial isolates, WGS successfully identified 96% of clinically relevant species and showed a high concordance rate of 99.3% for first-line drug resistance predictions.
- Despite similar processing times of around 20-21 days for both WGS and routine tests, there is still room for improvement in the accuracy of WGS predictions, particularly for cases with insufficient sequencing data.