N318L Blocks the Interaction of Fluralaner but Not Broflanilide or Fipronil with the Insect GABA Receptor .

Fluralaner is a novel insecticide targeting the ionotropic GABA receptor (GABAR) subunit, RDL. A recent study revealed that N316L, a substitution of asparagine (N) with leucine (L), in the second transmembrane (M2)-spanning region reduced the antagonist action of fluralaner on the housefly RDL (RDL) . To verify the impact of N316L , the corresponding mutation (N318L) in the fruitfly RDL (RDL) was constructed using CRISPR/Cas9 genome editing.