Rev Assoc Med Bras (1992)
February 2012
Objective: To evaluate factors associated to presence of free RNA-HIV in the vagina.
Methods: Cross-sectional study with HIV-infected women, excluding those who had undergone hysterectomy, had used vaginal medication within the last 48 hours, had had unprotected sex less than 72 hours before, were pregnant, or had genital bleeding. After signing an informed consent, blood samples were obtained for T CD4 lymphocytes count and plasmatic viral load, in addition to cervico-vaginal lavage using 10 mL of sterile normal saline, later centrifuged, aliquoted and stored at - 70 degrees C to quantify free HIV-RNA.