Advancing Source Tracking: Systematic Review and Source-Specific Genome Database Curation of Fecally Shed Prokaryotes.

Advancements within fecal source tracking (FST) studies are complicated by a lack of knowledge regarding the genetic content and distribution of fecally shed microbial populations. To address this gap, we performed a systematic literature review and curated a large collection of genomes (n = 26,018) representing fecally shed prokaryotic species across broad and narrow source categories commonly implicated in FST studies of recreational waters (i.e.

Conservation of Energetic Pathways for Electroautotrophy in the Uncultivated Candidate Order .

Electromicrobiology can be used to understand extracellular electron uptake in previously undescribed chemolithotrophs. Enrichment and characterization of the uncultivated electroautotroph " Tenderia electrophaga" using electromicrobiology led to the designation of the order Representative metagenome-assembled genomes (MAGs) have been identified in a number of environmental surveys, yet a comprehensive characterization of conserved genes for extracellular electron uptake has thus far not been conducted. Using comparative genomics, we identified conserved orthologous genes within the and nearest-neighbor orders important for extracellular electron uptake based on a previously proposed pathway from " Tenderia electrophaga.

Complete Genome Sequence of R2C4, Isolated from a Self-Regenerating Biocathode Consortium.

Here, we present the complete genome sequence of R2C4, isolated from the electroautotrophic microbial consortium biocathode MCL (--). As an isolate of a current-producing system, the genome sequence of will yield insights regarding electrode-associated microorganisms and communities. A dark pigment is also observed during cultivation.