Low-Fouling and Antibacterial Polymer Brushes via Surface-Initiated Polymerization of a Mixed Zwitterionic and Cationic Monomer.

The use of surface-grafted polymer brushes with combined low-fouling and antibacterial functionality is an attractive strategy to fight biofilm formation. This report describes a new styrene derivative combining a quaternary ammonium group with a sulfobetaine group in one monomer. Surface-initiated polymerization of this monomer on titanium and a polyethylene (PE) base material gave bifunctional polymer brush layers.

Different activation of MAPKs and Akt/GSK3β after preload vs. afterload elevation.

Aims: Pressure overload (PO) and volume overload (VO) lead to concentric or eccentric hypertrophy. Previously, we could show that activation of signalling cascades differ in in vivo mouse models. Activation of these signal cascades could either be induced by intrinsic load sensing or neuro-endocrine substances like catecholamines or the renin-angiotensin-aldosterone system.

The Bacteroidetes sp. and Produce Promiscuous Esterases With PET-Hydrolyzing Activity.

Certain members of the Actinobacteria and Proteobacteria are known to degrade polyethylene terephthalate (PET). Here, we describe the first functional PET-active enzymes from the Bacteroidetes phylum. Using a PETase-specific Hidden-Markov-Model- (HMM-) based search algorithm, we identified several PETase candidates from Flavobacteriaceae and Porphyromonadaceae.

Optimized production strategy of the major capsid protein HPV 16L1 non-assembly variant in E. coli.

The capsid of human papillomavirus (HPV) consists of two capsid proteins - the major capsid protein L1 and the minor capsid protein L2. Assembled virus-like particles, which only consist of L1 proteins, are successfully applied as prophylactic vaccines against HPV infections. The capsid subunits are L1-pentamers, which are also reported to protect efficiently against HPV infections in animals.

Influence of microorganism and plant oils on the structure of mannosylerythritol lipid (MEL) biosurfactants revealed by a novel thin layer chromatography mass spectrometry method.

Mannosylerythritol lipids (MEL) are microbial glycolipid biosurfactants with great potential for application in cosmetics and household detergents. In current biotechnological processes, they are produced by basidiomycetous fungi, the Ustilaginaceae, as a complex mixture of different chemical structures. It was the aim of this paper to study the influence of producer organisms and substrates on the resulting MEL structures with a novel high-resolution HPTLC-MALDI-TOF method.

Refolding and in vitro characterization of human papillomavirus 16 minor capsid protein L2.

The minor capsid protein L2 of papillomaviruses exhibits multiple functions during viral entry including membrane interaction. Information on the protein is scarce, because of its high tendency of aggregation. We determined suitable conditions to produce a functional human papillomavirus (HPV) 16 L2 protein and thereby provide the opportunity for extensive in vitro analysis with respect to structural and biochemical information on L2 proteins and mechanistic details in viral entry.

Differential cardiac remodeling in preload versus afterload.

Background: Hemodynamic load regulates myocardial function and gene expression. We tested the hypothesis that afterload and preload, despite similar average load, result in different phenotypes.

Methods And Results: Afterload and preload were compared in mice with transverse aortic constriction (TAC) and aortocaval shunt (shunt).

Memory, simple and complex language, and the temporal lobe.

Nineteen patients with intractable temporal lobe epilepsy who underwent anterior temporal lobectomy were given a highly specific memory battery (23 tests) pre- and post- (1 week; 1, 2, and 6 months; 1 and 2 years) resection. Sixteen of 23 tests revealed that memory performance of temporal lobe epilepsy patients was worse than normal controls prior to surgery (p < .001), while the most profound differences were seen in the remembering and generation of inferences from connected discourse.

Feeding interactions in infants with very low birth weight and bronchopulmonary dysplasia.

Infants with very low birth weight (VLBW) are at increased risk for feeding disorders that can affect growth and development. One hundred and forty one mother-infant pairs were compared [55 with infants with high medical risk due to infant VLBW and bronchopulmonary dysplasia (BPD), 34 VLBW without BPD, and 52 term infants] on operationally defined measures of feeding behaviors and maternal self-report of depression and anxiety. Mothers of VLBW infants with and without BPD spent more time prompting their infants to feed when their infants engaged in nonfeeding behavior.

Refractive index of some mammalian tissues using a fiber optic cladding method.

The index of refraction n of the many mammalian tissues is an important but somewhat neglected optical constant. Archival and oral papers have quoted the use of values of n for tissue generally ranging from 1.35 to 1.

Optical properties of mammalian tissue: introduction by the feature editors.

This issue of Applied Optics features twenty-two papers on the optical character of tissue, a topic of growing importance in the life sciences. The papers deal with theory and experiment and are contributed by a core of researchers active in the current development of this relatively new field. The complete description of the tissue's optical properties is fraught with problems due to the extraordinary nature of this difficult, varied, turbid, highly scattering medium.

Allergic reactions to systemic glucocorticoids: a review.

Over 30 cases of systemic reactivity to adrenocorticosteroids have been reported. With one exception, skin- and challenge-testing have indicated that the glucocorticoid, rather than its vehicle, has been the cause of the reaction. Aspirin-sensitive asthmatic patients may be particularly predisposed to reactions.

Long-term alternate day steroid therapy in renal transplantation. A controlled study.

Seventy-six adult renal allograft recipients were allocated 5 months post-transplantation to daily or alternate day maintenance methylprednisolone therapy. All 15 recipients of living related kidneys and 23 recipients of cadaver kidneys were placed on the alternate day regimen, while 38 patients with cadaveric grafts remained on daily methylprednisolone. In patients on alternate day methylprednisolone, serum creatinine concentrations, frequency of acute rejection episodes, and prevalence of chronic rejection were similar to those of patients on daily steroids.