Publications by authors named "Prerna Mudai"
o-Vanillin binds covalently to MAL/TIRAP Lys-210 but independently inhibits TLR2.
J Enzyme Inhib Med Chem
December 2024
Article Synopsis
- TLR signaling is crucial for innate immunity against pathogens but can lead to inflammation if overly activated, highlighting the need for specific inhibitors.
- The small molecule o-vanillin has been identified as an inhibitor of TLR2 signaling, binding to and disrupting the assembly of the TLR adaptor protein MAL.
- Research shows that o-vanillin forms a covalent bond with MAL and inhibits TLR2 exclusively, indicating it might modify TLR2 signaling complexes directly, potentially affecting various proteins in cells.
Caspase-1 location in cells has been studied with fluorochrome-labeled inhibitors of caspase-1 (FLICA reagents). We report that FLICA reagents have limited cell-membrane permeability. This impacts experimental design as cells with intact membranes, including caspase-1 knockout cells, are not appropriate controls for cells with inflammasome-induced gasdermin D membrane pores.
View Article and Find Full Text PDF