Single nucleotide polymorphism in sugar pathway and disease resistance genes in sugarcane.

Single nucleotide polymorphism in sugar pathway and disease resistance genes showing genetic association with sugar content and red rot resistance would be useful in marker-assisted genetic improvement of sugarcane. Validation and genotyping of potential sequence variants in candidate genes are necessary to understand their functional significance and trait association potential. We discovered, characterized, validated and genotyped SNPs and InDels in sugar pathway and disease resistance genes of Saccharum complex and sugarcane varieties using amplicon sequencing and CAPS assays.

Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine.

Convolvulus pluricaulis Choisy, commonly known as "Shankhpushpi", is an ayurvedic medicinal plant recommended as a brain tonic to promote intellect and memory, eliminate nervous disorders and to treat hypertension. Because of increasing demand of the drug, this plant species has been over-exploited. As a consequence, many unrelated plants are being sold by the crude drug dealers in India in the name of "Shankhpushpi".

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering.

High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B.

Modulation of endogenous pathways enhances bioethanol yield and productivity in Escherichia coli.

Background: E. coli is a robust host for various genetic manipulations and has been used commonly for bioconversion of hexose and pentose sugars into valuable products. One of the products that E.

Characterization of stress and methylglyoxal inducible triose phosphate isomerase (OscTPI) from rice.

As compared with plant system, triose phosphate isomerase (TPI), a crucial enzyme of glycolysis, has been well studied in animals. In order to characterize TPI in plants, a full-length cDNA encoding OscTPI was cloned from rice and expressed in E. coli.

Molecular markers in medicinal plant biotechnology: past and present.

Plant based medicines have gained popularity worldwide due to their almost negligible side effects. In India, the three traditional medicinal systems, namely homeopathy, Ayurveda and Siddha rely heavily on plants for medicinal formulations. To prevent the indiscriminate collection of these valuable medicinal plants and for their proper authentication and conservation, it is imperative to go for sustained efforts towards proper germplasm cataloguing and devising conservation strategies.

Functionally relevant microsatellites in sugarcane unigenes.

Background: Unigene sequences constitute a rich source of functionally relevant microsatellites. The present study was undertaken to mine the microsatellites in the available unigene sequences of sugarcane for understanding their constitution in the expressed genic component of its complex polyploid/aneuploid genome, assessing their functional significance in silico, determining the extent of allelic diversity at the microsatellite loci and for evaluating their utility in large-scale genotyping applications in sugarcane.

Results: The average frequency of perfect microsatellite was 1/10.

Informative genomic microsatellite markers for efficient genotyping applications in sugarcane.

Genomic microsatellite markers are capable of revealing high degree of polymorphism. Sugarcane (Saccharum sp.), having a complex polyploid genome requires more number of such informative markers for various applications in genetics and breeding.