The interaction of galectin-8 C-terminal domain with cell surface glycoconjugates modulates membrane elasticity to stimulate antigen uptake and presentation to CD4 T cells.

Galectins constitute a family of soluble lectins with unique capacity to induce macroscale rearrangements upon interacting with cell membrane glycoconjugates. Galectin-8 (Gal-8) is acknowledged for its role in facilitating antigen uptake and processing upon engaging with cell surface glycoconjugates on antigen-presenting cells (APCs). Gal-8 consists of two covalently fused N- and C-terminal carbohydrate recognition domains (N- and C-CRD), each exhibiting distinct glycan specificity.

Investigating yielding behavior of heterogeneous vehicles at a semi-controlled crosswalk.

It is well known that pedestrians are vulnerable road users. Their risk of being injured or killed in road traffic crashes is even higher as vehicle drivers often violate traffic rules and do not slow down or yield in front of crosswalks. In order to reduce this risk, many countries have issued strict regulations requiring vehicles to yield to pedestrians in front of crosswalks.

Synergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection.

Toxoplasmosis is a zoonotic disease with worldwide prevalence in humans and warm-blooded animal populations. In livestock Toxoplasma gondii is the causal agent of significant economic losses since it can cause abortions in goats and sheep. It is estimated that one third of the world population is infected.

Optimized Adenoviral Vector That Enhances the Assembly of FMDV O1 Virus-Like Particles Increases Its Potential as Vaccine for Serotype O Viruses.

Although replication-defective human adenovirus type 5 (Ad5) vectors that express the capsid-encoding region of foot-and-mouth disease virus (FMDV) have been proven to be effective as vaccines in relevant species for several viral strains, the same result was not consistently achieved for the O1/Campos/Brazil/58 strain. In the present study, an optimization of the Ad5 system was explored and was proven to enhance the expression of FMDV capsid proteins and their association into virus-like particles (VLPs). Particularly, we engineered a novel Ad5 vector (Ad5[P]) which harbors the foreign transcription unit in a leftward orientation relative to the Ad5 genome, and drives the expression of the FMDV sequences from an optimized cytomegalovirus (CMV) enhancer-promoter as well.

Ranking contributors to traffic crashes on mountainous freeways from an incomplete dataset: A sequential approach of multivariate imputation by chained equations and random forest classifier.

The estimation of the effect of contributors to crash injury severity and the prediction of crash injury severity outcomes suffer often from biases related to missing data in crash datasets that contain incomplete records. As both estimation and prediction would greatly improve if the missing values were recovered, this study proposes a sequential approach to handle incomplete crash datasets and rank contributors to the injury severity of crashes on mountainous freeways in China. The sequential approach consists of two parts: (i) multivariate imputation by chained equations imputes the missing values of independent variables; (ii) a random forest classifier analyses the correlation between the dependent and the independent variables.

Galectin-8 Enhances T cell Response by Promotion of Antigen Internalization and Processing.

Galectin-8 (Gal-8) is a mammalian lectin endowed with immunostimulatory ability. In the present work, we demonstrate that Gal-8-glycan interactions on the surface of antigen-presenting cells (APCs) promote antigen binding and internalization, independently from antigen nature. Both Gal-8 and antigen were together internalized and localized in early endosomes.

A bivariate random effects spatial model of traffic fatalities and injuries across Provinces of Iran.

From 2005-2015, Iran has experienced a 41.3 % decrease in road fatalities and an 11.1 % increase in non-fatal injuries.

Galectin-8 in the onset of the immune response and inflammation.

Galectins (Gals), a family of mammalian lectins, have emerged as key regulators of the immune response, being implicated in several physiologic and pathologic conditions. Lately, there is increasing data regarding the participation of Galectin-8 (Gal-8) in both the adaptive and innate immune responses, as well as its high expression in inflammatory disorders. Here, we focus on the pro- and anti-inflammatory properties of Gal-8 and discuss the potential use of this lectin in order to shape the immune response, according to the context.

Nidogen-1 is a novel extracellular ligand for the NKp44 activating receptor.

The release of soluble ligands of activating Natural Killer (NK) cell receptors may represent a regulatory mechanism of NK cell function both in physiologic and in pathologic conditions. Here, we identified the extracellular matrix protein Nidogen-1 (NID1) as a ligand of NKp44, an important activating receptor expressed by activated NK cells. When released as soluble molecule, NID1 regulates NK cell function by modulating NKp44-induced IFN-γ production or cytotoxicity.

Interleukin-6 signalling mediates Galectin-8 co-stimulatory activity of antigen-specific CD4 T-cell response.

Galectin-8 (Gal-8) is a mammalian lectin endowed with the ability to co-stimulate antigen-specific immune responses. We have previously demonstrated that bone-marrow-derived dendritic cells produce high levels of interleukin-6 (IL-6) in response to Gal-8 stimulation. As IL-6 is a pleiotropic cytokine that has a broad effect on cells of the immune system, we aimed to elucidate whether IL-6 was involved in Gal-8-dependent co-stimulatory signals during antigen recognition by specific CD4 T cells.

The relation between working conditions, aberrant driving behaviour and crash propensity among taxi drivers in China.

Although the taxi industry is playing an important role in Chinese everyday life, little attention has been posed towards occupational health issues concerning the taxi drivers' working conditions, driving behaviour and road safety. A cross-sectional survey was administered to 1021 taxi drivers from 21 companies in four Chinese cities and collected information about (i) sociodemographic characteristics, (ii) working conditions, (iii) frequency of daily aberrant driving behaviour, and (iv) involvement in property-damage-only (PDO) and personal injury (PI) crashes over the past two years. A hybrid bivariate model of crash involvement was specified: (i) the hybrid part concerned a latent variable model capturing unobserved traits of the taxi drivers; (ii) the bivariate part modelled jointly both types of crashes while capturing unobserved correlation between error terms.

Contributors to young drivers' driving styles - A comparison between Israel and Queensland.

Among the numerous factors that contribute to young novice drivers' driving styles, personality characteristics, sociodemographic variables, family atmosphere, and friends' norms are known to have an important impact. However, cross-cultural comparisons are relatively rare in the safety literature concerning young drivers. This study aimed at comparing young drivers from Israel and Queensland (Australia) and examining the contribution of personality, sociodemographic, family and friends' aspects to their driving styles (reckless and careless; hostile and angry; anxious; patient and careful).

Considering built environment and spatial correlation in modeling pedestrian injury severity.

Objective: This study looks at mitigating and aggravating factors that are associated with the injury severity of pedestrians when they have crashes with another road user and overcomes existing limitations in the literature by focusing attention on the built environment and considering spatial correlation across crashes.

Method: Reports for 6,539 pedestrian crashes occurred in Denmark between 2006 and 2015 were merged with geographic information system resources containing detailed information about the built environment and exposure at the crash locations. A linearized spatial logit model estimated the probability of pedestrians sustaining a severe or fatal injury conditional on the occurrence of a crash with another road user.

The Innate Immune Cross Talk between NK Cells and Eosinophils Is Regulated by the Interaction of Natural Cytotoxicity Receptors with Eosinophil Surface Ligands.

Previous studies suggested that the cross talk between NK cells and other cell types is crucial for the regulation of both innate and adaptive immune responses. In the present study, we analyzed the phenotypic and functional outcome of the interaction between resting or cytokine-activated NK cells and eosinophils derived from non-atopic donors. Our results provide the first evidence that a natural cytotoxicity receptor (NCR)/NCR ligand-dependent cross talk between NK cells and eosinophils may be important to upregulate the activation state and the effector function of cytokine-primed NK cells.

How indicative is a self-reported driving behaviour profile of police registered traffic law offences?

Although most motorised countries have experienced massive improvements in road safety over the last decades, human behaviour and differences in accident risk across sub-groups of drivers remains a key issue in the area of road safety. The identification of risk groups requires the identification of reliable predictors of safe or unsafe driving behaviour. Given this background, the aim of this study was to test whether driver sub-groups identified based on self-reported driving behaviour and skill differed in registered traffic law offences and accidents, and whether group membership was predictive of having traffic law offences.

Understanding traffic crash under-reporting: Linking police and medical records to individual and crash characteristics.

Objective: This study aligns to the body of research dedicated to estimating the underreporting of road crash injuries and adds the perspective of understanding individual and crash factors contributing to the decision to report a crash to the police, the hospital, or both.

Method: This study focuses on road crash injuries that occurred in the province of Funen, Denmark, between 2003 and 2007 and were registered in the police, the hospital, or both authorities. Underreporting rates are computed with the capture-recapture method, and the probability for road crash injuries in police records to appear in hospital records (and vice versa) is estimated with joint binary logit models.

Profiling drunk driving recidivists in Denmark.

Drunk drivers are a menace to themselves and to other road users, as drunk driving significantly increases the risk of involvement in road accidents and the probability of severe or fatal injuries. Although injuries and fatalities related to road accidents have decreased in recent decades, the prevalence of drunk driving among drivers killed in road accidents has remained stable, at around 25% or more during the past 10 years. Understanding drunk driving, and in particular, recidivism, is essential for designing effective countermeasures, and accordingly, the present study aims at identifying the differences between non-drunk drivers, drunk driving non-recidivists and drunk driving recidivists with respect to their demographic and socio-economic characteristics, road accident involvement and other traffic and non-traffic-related law violations.

Fatal and serious road crashes involving young New Zealand drivers: a latent class clustering approach.

The over-representation of young drivers in road crashes remains an important concern worldwide. Cluster analysis has been applied to young driver sub-groups, but its application by analysing crash occurrence is just emerging. We present a classification analysis that advances the field through a holistic overview of crash patterns useful for designing youth-targeted road safety programmes.

Observed and unobserved correlation between crash avoidance manoeuvers and crash severity.

Understanding drivers' responses to critical events, analyzing drivers' abilities to perform corrective manoeuvers, and investigating the correlation between these manoeuvers and crash severity provide the opportunity of increasing the knowledge about how to avoid crash occurrence or at least mitigate crash severity. We extend existing research on the determinants of engaging in crash avoidance manoeuvers by considering that observable and unobservable factors relate to both the selection of corrective manoeuvers and the severity outcome. Accordingly, we propose a joint multinomial-logit ordered-probit model of single-vehicle crashes extracted from the NASS GES database for the years 2005-2009.

Influence of the built environment on pedestrian route choices of adolescent girls.

We examined the influence of the built environment on pedestrian route selection among adolescent girls. Portable global positioning system units, accelerometers, and travel diaries were used to identify the origin, destination, and walking routes of girls in San Diego, CA and Minneapolis, MN. We completed an inventory of the built environment on every street segment to measure the characteristics of routes taken and not taken.

Parents' and peers' contribution to risky driving of male teen drivers.

The current study joins efforts devoted to understanding the associations of parents' personality, attitude, and behavior, and to evaluating the added contribution of peers to the driving behavior of young drivers during their solo driving. The study combines data gathered using in-vehicle data recorders from actual driving of parents and their male teen driver with data collected from self-report questionnaires completed by the young drivers. The sample consists of 121 families, who participated in the study for 12 months, beginning with the licensure of the teen driver.

Aggravating and mitigating factors associated with cyclist injury severity in Denmark.

Introduction: Denmark is one of the leading cycling nations, where cycling trips constitute a large share of the total trips, and cycling safety assumes a top priority position in the agenda of policy makers. The current study sheds light on the aggravating and mitigating factors associated with cyclist injury severity on Danish roads by examining a comprehensive set of accidents involving a cyclist and a collision partner between 2007 and 2011.

Method: This study estimates a generalized ordered logit model of the severity of cyclist injuries because of its ability to accommodate the ordered-response nature of severity while relaxing the proportional odds assumption.

Analysis of factors associated with injury severity in crashes involving young New Zealand drivers.

Young people are a risk to themselves and other road users, as motor vehicle crashes are the leading cause of their death. A thorough understanding of the most important factors associated with injury severity in crashes involving young drivers is important for designing well-targeted restrictive measures within youth-oriented road safety programs. The current study estimates discrete choice models of injury severity of crashes involving young drivers conditional on these crashes having occurred.

Cyclist-motorist crash patterns in Denmark: a latent class clustering approach.

Objective: The current study aimed at uncovering patterns of cyclist-motorist crashes in Denmark and investigating their prevalence and severity. The importance of implementing clustering techniques for providing a holistic overview of vulnerable road users' crash patterns derives from the need to prioritize safety issues and to devise efficient preventive measures.

Method: The current study focused on cyclist-motorist crashes that occurred in Denmark during the period between 2007 and 2011.