Stability and Fibrillation of Lysozyme in the Mixtures of Ionic Liquids with Varying Hydrophobicity.

Combinatorial effects of small molecules provide newer avenues to improve protein stability. The combined effect of two different classes of ILs on the stability and fibrillation propensity of lysozyme (Lyz) was investigated. Imidazolium-ILs (an aromatic moiety) with varying alkyl chains, methyl (MIC), butyl (BMIC) and hexyl (HMIC), and pyrrolidinium-IL (alicyclic moiety) with butyl substitution (BPyroBr) were chosen.