Publications by authors named "Prasiddha Guragain"
Three-dimensional (3D) tumor models provide physiologically relevant tumor environments and have become a major tool in cancer research and drug discovery. This article presents a protocol for creating a 3D organotypic tumor model by embedding a cancer cell spheroid within a collagen matrix containing dispersed fibroblasts. This model offers significant advantages over the conventional monolayer cell culture, monoculture spheroids of cancer cells, and intermixed co-culture of cancer and stromal cells by mimicking the spatial organization and mechanical properties of a solid tumor.
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- Tissue chip and organs-on-chip technologies are becoming important in preclinical cancer research due to the high failure rates of new drugs in clinical trials.
- A new 32-unit tissue chip designed to fit standard 96-well plates simplifies automation and uses 3D printing and gravity-driven flow for better functionality.
- This tissue chip allows for the simultaneous analysis of drug efficacy and toxicity, focusing on liver and bone marrow interactions, thereby improving the selection of potential treatments in early drug discovery stages.