Publications by authors named "Prasanta Kumar K Mishra"
Diagnosis of Human Leptospirosis: Comparison of Microscopic Agglutination Test with Recombinant LigA/B Antigen-Based In-House IgM Dot ELISA Dipstick Test and Latex Agglutination Test Using Bayesian Latent Class Model and MAT as Gold Standard.
Diagnostics (Basel)
June 2022
Article Synopsis
- Leptospirosis is a widespread spirochaetal infection that affects a variety of mammals, and this study compares diagnostic methods for detecting it in humans.
- The research evaluates the microscopic agglutination test (MAT) against two point-of-care tests: the IgM dot ELISA dipstick test (IgM-DED) and the latex agglutination test (LAT), using Bayesian latent class modeling to analyze their effectiveness.
- Results show that the IgM-DED and LAT provide high sensitivity for early detection of leptospirosis, outperforming the single MAT test, and suggest that these point-of-care tests are practical for use in remote areas, while combining acute and convalescent samples significantly boosts MAT sensitivity.
Introduction: Parasites release a wide array of protein as excretory and secretory products (ESPs). Irrespective of their mode of propagation, ESPs are found to be secreted or excreted by both naturally occurring and laboratory-cultivated parasites. Mass spectrometry-based approaches have been extensively used to identify and characterize the ESP constituents.
View Article and Find Full Text PDFLeptospirosis is responsible for hampering the productivity of swine husbandry worldwide. The aim of this study was to assess the efficacy of bioinformatics tools in predicting the three-dimensional structure and immunogenicity of recombinant LigBCon1-5 (rLigBCon1-5) antigen. A battery of bioinformatics tools such as I-TASSER, ProSA and SAVES v6.
View Article and Find Full Text PDFIntroduction: Haemonchus contortus is an economically important parasite of domestic animals. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) excreted in the ES product of H. contortus can be a promising vaccine candidate for controlling the parasite infection.
View Article and Find Full Text PDFIntraphagocytic survival of Salmonella Typhimurium (ST) depends (at least in part) upon its ability to repair oxidant-damaged macromolecules. Met residues either free or in protein bound form are highly susceptible to phagocyte-generated oxidants. Oxidation of Mets leads to Met-SO formation, consequently loss of protein functions that results in cell death.
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