Publications by authors named "Prasad P Loni"
J Basic Microbiol
October 2014
Article Synopsis
- An alkaline chitinase was isolated and purified from the bacterium Paenibacillus pasadenensis NCIM 5434 found in the alkaline soil of Lonar Lake using ammonium sulfate precipitation and DEAE cellulose column chromatography, achieving 8.87-fold purification with 24.96% yield.
- Molecular analysis showed the enzyme has a molecular weight of about 35 kDa, with kinetic parameters indicating a Km of 6.25 mg/ml and Vmax of 434.78 µM for colloidal chitin, operating optimally at pH 10 and 37°C.
- The enzyme displays notable antifungal activity against Penicillium and Aspergillus, suggesting its potential
