In the present work, enzymes pectinase and naringinase were simultaneously co-immobilized on an eco-friendly chitosan coated magnetic nanoparticles (chitosanMNPs) by cross-linking using chitosan as a macro-molecular cross-linker. The maximum activity recovery of both enzymes in the co-immobilized form was obtained at chitosanMNPs to enzymes ratio of 1:3, 3% cross-linker concentration and 150 min cross-linking time. The synthesized MNPs before and after co-immobilization were characterized using different techniques.
View Article and Find Full Text PDFIn the present work, laccase was successfully immobilized in peroxidase mimicking magnetic metal organic frameworks (MMOFs) within 30 min using a facile approach. The integration of magnetic nanoparticles during synthesis significantly eases the separation of prepared biocatalyst using an external magnet. The immobilization of laccase was confirmed using different characterization techniques.
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