Evaluation of the microbial wet-vacuum system (M-Vac®) for DNA sampling from rough, porous substrates, and its compatibility with fully automated platforms.

DNA retrieval methods traditionally used during forensic evidence recovery including swabbing and tape lifting, can have limited effectiveness when used on porous, rough substrates such as bricks and carpet. This is possibly due to the DNA material being dispersed and unreachable for surface sampling techniques. In this evaluation we investigated the effectiveness of the Microbial Wet-Vacuum System (M-Vac®; M-Vac® Systems, Inc.

Aggregation induced emission and volatile acid vapour sensing in acridine appended poly (aryl ether) based low molecular weight organogelator.

Considerable research attention has been devoted to the development of portable and rapid fluorescence sensors that can selectively detect volatile acids, due to the harmful effects of acid vapour on the environment and human health. Although various types of fluorophores have been reported for sensing volatile acid vapours, regulation of the sensory response using aggregation induced emissive (AIE) based gelators has rarely been reported. In this study, we present the design and synthesis of a novel organogelator that is capable of sensing volatile acids through AIE.

A bacteriorhodopsin-based biohybrid solar cell using carbon-based electrolyte and cathode components.

There is currently a high demand for energy production worldwide, mainly producing renewable and sustainable energy. Bio-sensitized solar cells (BSCs) are an excellent option in this field due to their optical and photoelectrical properties developed in recent years. One of the biosensitizers that shows promise in simplicity, stability and quantum efficiency is bacteriorhodopsin (bR), a photoactive, retinal-containing membrane protein.

A Gelation-Induced Enhanced Emission Active Stimuli Responsive and Superhydrophobic Organogelator: "Turn-On" Fluorogenic Detection of Cyanide and Dual-Channel Sensing of Nitroexplosives on Multiple Platforms.

A pyrene-based highly emissive low-molecular-weight organogelator, [2-(4-fluorophenyl)-3-(pyren-1-yl)acrylonitrile] (F1), is presented, which divulges thixotropic and thermochromic fluorescence switching via reversible gel-to-sol transition and tremendous superhydrophobicity (mean contact angles: 149-160°), devoid of any gelling and/or hydrophobic units. The rationale for the design strategy reveals that the restricted intramolecular rotation (RIR) in J-type self-assembly promotes F1 for the prolific effects of aggregation- and gelation-induced enhanced emission (AIEE and GIEE). Meanwhile, hindrance in charge transfer via the nucleophilic reaction of cyanide (CN) on the C═C unit in F1 facilitates the selective fluorescence "turn-on" response in both solution [9:1 (v/v) DMSO/water] and solid state [paper kits] with significantly lower detection limits (DLs) of 37.

Development of a rapid process for purification of Bowman-Birk and Kunitz inhibitors from legume seeds, and evaluation of their biophysical, insecticidal, and antimicrobial properties.

Bowman-Birk inhibitor (BBI ~10 kDa) and Kunitz inhibitor (KI ~20 kDa) are serine protease/proteinase inhibitor(s) [PI(s)] ubiquitously found in several Leguminous plant species with insecticidal and therapeutic properties. Due to narrow molecular mass differences, the separation of these inhibitors from a single seed variety is tedious. The present study is aimed to develop a rapid protocol (<24 h) for purifying BBI and KI from legume seeds using mild trichloroacetic acid (TCA) extraction followed by trypsin-affinity chromatography.

Effect of positional isomerism on the excited state charge transfer dynamics of anthracene-based D-π-A systems.

Understanding the dynamics of the back electron transfer (BET) rate of ion pairs from the electronically excited state of donor-acceptor systems is crucial for developing materials for organic electronics. The structure-property relationships in the organic molecular architectures play a key role in controlling the BET rate and have been utilized as a criterion to design systems with a reduced BET rate. Here, we examine the influence of isomerism on the BET rate in anthracene based systems, , ()-2-(2-(anthracen-9-yl)vinyl)benzonitrile (-CN) and ()-3-(2-(anthracen-9-yl)vinyl)benzonitrile (-CN) with ,-diethylaniline (DEA) in methylcyclohexane using time-resolved spectroscopy.

Electronic effect of substituents on the charge-transfer dynamics at the CsPbBr perovskite-small molecule interface.

To push the boundary of the efficiency of perovskite nanocrystal-based photovoltaics, understanding the charge transfer at the interface of these nanocrystals is necessary. In an effort to understand the electronic effects of the substituents in the charge acceptor moiety, three electronically different small molecules (namely, chloranilic acid (CA), -benzoquinone (BQ), and duroquinone (DQ)) were chosen and their detailed charge transfer dynamics were studied at the CsPbBr perovskite nanocrystal-small organic molecule interface using steady state and time-resolved spectroscopic methods. The steady-state absorption and time-resolved emission studies reveal that all three molecules interact with the NCs in the excited state.

Are patients receiving recommended bone protection therapy after a non-hip fracture? A retrospective study of the Fracture Liaison Service at Counties Manukau District Health Board.

Aim: To review the Fracture Liaison Service (FLS) recommendations for bone protection therapy and assess treatment implementation in the community.

Method: All patients screened from 1 January to 31 March 2019 at Counties Manukau District Health Board were evaluated. Exclusion criteria included death within six months following sentinel fracture, and hip fractures, which are studied elsewhere.

Image Processing and Machine Learning-Based Classification and Detection of Liver Tumor.

The liver is in charge of a plethora of tasks that are critical to healthy health. One of these roles is the conversion of food into protein and bile, which are both needed for digestion. Inhaled and possibly harmful chemicals are flushed from the body.

Crystal Structure and Twisted Aggregates of Oxcarbazepine Form III.

Polymorphism and crystal habit play vital roles in dictating the properties of crystalline materials. Here, the structure and properties of oxcarbazepine (OXCBZ) form III are reported along with the occurrence of twisted crystalline aggregates of this metastable polymorph. OXCBZ III can be produced by crystallization from the vapor phase and by recrystallization from solution.

Manufacture of tablets with structurally-controlled drug release using rapid tooling injection moulding.

With advancements in the pharmaceutical industry pushing more towards tailored medicines, novel approaches to tablet manufacture are in high demand. One of the main drivers towards micro-scale batch production is the ability to fine-tune drug release. This study demonstrates the use of rapid tooling injection moulding (RTIM) for tablet manufacture.

5FU encapsulated polyglycerol sebacate nanoparticles as anti-cancer drug carriers.

The majority of anti-cancer drugs fail to reach clinical trials due to their low water solubility. A biocompatible drug delivery system that encapsulates and efficiently delivers hydrophobic drugs to the target site is the need of the hour. This study addresses the issue by focusing on a polymeric polyglycerol sebacate (PGS) nanoparticles loaded with 5-fluorouracil (5FU), a primary line chemotherapy drug for many types of cancers.

Mefenamic acid solid dispersions: Impact of formulation composition on processing parameters, product properties and performance.

The objective of this study was to develop an immediate release (IR), crystalline solid dispersion (CSD) formulation of Mefenamic acid (MFA) by hot-melt-extrusion (HME) and assess the impact of drug loading on process parameters, product physico-chemical properties and product performance. An HME process to produce a range of MFA-Soluplus®-Sorbitol polymer matrix CSD formulations was developed based on rheological screening assays of physical mixtures (PM). The impact of drug loading on process parameters was compared to the impact of drug loading on the physico-chemical properties of formulations.

Molecular Surveillance and Prediction of Antimicrobial Resistance of Neisseria gonorrhoeae in Northern Alberta, Canada, 2015 to 2018.

Background: The aims of this study was to describe molecular surveillance of Neisseria gonorrhoeae in the North Zone of Alberta (NZ) and to determine its value in predicting antimicrobial resistance.

Methods: Sequence types (STs) and single-nucleotide polymorphism (SNP) assays were performed on nucleic acid amplification testing (NAAT) samples. Sequence types of NAATs were matched to ST of cultures from across Alberta.

Touch DNA recovery from unfired and fired cartridges: Comparison of swabbing, tape lifting and soaking.

Over the recent few years, several DNA collection techniques and methodologies have been published for the recovery of DNA from fired cartridge cases. In this study, swabbing, the DNA collection technique currently used in our jurisdiction (NSW, Australia), was compared with tape lifting and soaking to assess DNA recovery rates, DNA quality and profile quality. Brass .

Modular amphiphilic poly(aryl ether)-based supramolecular nanomicelles: an efficient endocytic drug carrier.

A rationally designed amphiphilic poly(aryl ether)-based dendrimer self-assembles into nanomicelles and exhibits tunable morphology upon varying the hydrophilic chain length. The 30 nm-sized dendrimer nanomicelles successfully entrapped Doxorubicin, demonstrated the sustained release of Doxorubicin and can successfully penetrate cancer cells through caveolae-dependent endocytosis, compared to the free drug.

Insights into the Formations of Host-Guest Complexes Based on the Benzimidazolium Based Ionic Liquids-β-Cyclodextrin Systems.

Inclusion complexation is one of the best strategies for developing a controlled release of a toxic drug without unexpected side effects from the very beginning of the administration to the target site. In this study, three benzimidazolium based ionic liquids (ILs) having bromide anion and cation bearing long alkyl chains, hexyl- ([CCFBim]Br), octyl- ([CCFBim]Br), and decyl- ([CCFBim]Br) were designed and synthesized as antibacterial drugs. Inclusion complexes (ICs) of studied ILs have been prepared by the combination of β-cyclodextrin (β-CD), considering these conjugations should enhance the benignity of ILs and make them potential candidates for the controlled drug release.

Current Therapies in Clinical Trials of Parkinson's Disease: A 2021 Update.

Parkinson's disease (PD) is a progressive neurodegenerative disorder that currently has no cure, but treatments are available to improve PD symptoms and maintain quality of life. In 2020, about 10 million people worldwide were living with PD. In 1970, the United States Food and Drug Administration approved the drug levodopa as a dopamine replacement to manage PD motor symptoms; levodopa-carbidopa combination became commercialized in 1975.

Molecular Characterization and Antimicrobial Resistance in Neisseria gonorrhoeae, Nunavut Region of Inuit Nunangat, Canada, 2018-2019.

We assessed antimicrobial resistance (AMR) in Neisseria gonorrhoeae in Nunavut, Canada, using remnant gonorrhea nucleic acid amplification test-positive urine specimens. This study confirms the feasibility of conducting N. gonorrhoeae AMR surveillance and highlights the diversity of gonococcal sequence types and geographic variation of AMR patterns in the territory.

Development of a time of flight spectrometer based on position sensitive multi-wire proportional counters for fission fragment mass distribution studies.

Characteristics and performance of a time of flight (TOF) spectrometer developed for performing fission mass distribution studies are presented. The spectrometer contains two TOF arms based on multi-wire proportional counters (MWPCs). Each arm has two MWPCs to form a start-stop detection system for TOF measurements.

Behavioral Tests in Neurotoxin-Induced Animal Models of Parkinson's Disease.

Currently, neurodegenerative diseases are a major cause of disability around the world. Parkinson's disease (PD) is the second-leading cause of neurodegenerative disorder after Alzheimer's disease. In PD, continuous loss of dopaminergic neurons in the substantia nigra causes dopamine depletion in the striatum, promotes the primary motor symptoms of resting tremor, bradykinesia, muscle rigidity, and postural instability.

DNA recovery from unfired and fired cartridge cases: A comparison of swabbing, tape lifting, vacuum filtration, and direct PCR.

The ability to recover trace DNA from fired cartridge cases can help establish important leads regarding the handler of the ammunition. Over recent years, several DNA recovery techniques for fired ammunition have been published. Three techniques of significant interest include tape lifting, direct PCR, and vacuum filtration.

Improving Consistency for a Mefenamic Acid Immediate Release Formulation.

The objective of this study was to develop an immediate release dose form containing 250 mg Mefenamic acid (MFA) presented as a crystalline solid dispersion in order to achieve improved consistency in drug release through a simplified formulation compared to a commercial product. An MFA-Soluplus®-Sorbitol polymer matrix was developed using an HME process based on rheological screening assays of physical mixtures. The physico-chemical properties of these formulations were assessed by thermal analysis, FTIR, mechanical testing and SEM image analysis, confirming the crystalline character and stable polymorphic form I of the API in the polymer matrix.

Cyanide Sensing in Water Using a Copper Metallogel through "Turn-on" Fluorescence.

The development of probes for selective detection of cyanide has gained considerable attention over the past two decades due to benefits like high selectivity as well as sensitivity, fast response, visual output, accurate quantification, and a simplified sample preparation procedure. However, the propensity of toward fluorescence sensing of cyanide in aqueous medium is not well explored until now. Herein, we report the design and synthesis of a novel copper based metallogel capable of sensing cyanide in water by fluorescence "turn on".