Overcoming microbial resuscitation using stable ultrafine gold nanosystems.

While ultrafine gold nanosystems (UGNs) are being extensively studied for their antimicrobial activities, hitherto, no report is available on their propensity towards mitigating bacterial resuscitation-a potential factor contributing to the antimicrobial resistance. The investigations herein with two categories of gold nanosystems-modulated for their stability and surface accessibility through glutathione capping-have provided insights into overcoming resuscitation. Additionally, the study cautions that even moderate resistance development in bacteria exposed to nanosystems can result in significant cross-resistance against conventional antibiotics.

Probing the Effects of Antimicrobial-Lysozyme Derivatization on Enzymatic Degradation of Poly(ε-caprolactone) Film and Fiber.

Surface derivatization is essential for incorporating unique functionalities into biodegradable polymers. Nonetheless, its precise effects on enzymatic biodegradation still lack comprehensive understanding. In this study, a facile solution-based method is employed to surface derivatize poly(ε-caprolactone) films and electrospun fibers with lysozyme, aiming to impart antimicrobial properties and examine the impact on enzymatic degradation.

Direct experimental evidence for the boronic acid-mediated bridging of DNA hybrids.

The use of terminal deoxynucleotidyl transferase for the first time in a mechanistic exploration-through colorimetric sensing and isothermal titration calorimetric studies-has provided direct experimental evidence of a boronic acid moiety bridging two DNA duplexes the 3' hydroxyl groups, offering new opportunities and insights into the domain of DNA (nano)biotechnology.

Boronic acid chemistry for fluorescence-based quantitative DNA sensing.

Contrary to the long-standing opinion of boronic acids being typically reactive with 1,2- and 1,3-diols and hence not suitable for quantitative sensing of DNA containing only a mono-ol unit, this proof-of-concept study has successfully shown the feasibility to quantitatively detect DNA in the concentration range of 5 to 50 nM plausibly through boronic acid-mediated bridging of two DNA double helices the 3' hydroxy groups, which opens up new avenues in the realm of oligonucleotide biochemistry.

Fluorescence-based simultaneous dual oligo sensing of HCV genotypes 1 and 3 using magnetite nanoparticles.

Nucleic acid tests (NATs) have gained an important position in biosensing in the context of the increasing need to meet the stringent requirements for accurate diagnosis of infectious diseases with high sensitivity and selectivity. Recently, the development of new strategies towards multiplex detection of analytes in a single assay is gaining impetus since such an approach would lead to high throughput analysis, leading to substantial benefits in terms of time, infrastructure, labor, and cost. In this work, we demonstrate a facile fluorescence-based simultaneous dual oligo sensing of genotypes 1 and 3 by employing two target sequences (36-mers each) derived from the NS4B and NS5A regions of HCV genome, respectively.

Quaternized Polydopamine Coatings for Anchoring Molecularly Dispersed Broad-Spectrum Antimicrobial Silver Salts.

Because of the broad-spectrum antimicrobial efficacy, silver-based coatings have emerged as the popular choice to apply over frequently touched surfaces for mitigating the spread of nosocomial infections. Despite the advancements through various coating strategies, clustering of the active component remains a bottleneck in achieving the molecular-scale dispersion of silver. To circumvent this, the current study takes advantage of the recent findings of quaternary ammonium moieties forming molecular complexes with silver salts that differ from the simple adduct between the individual components.

Water-soluble caffeic acid-dopamine acid-base complex exhibits enhanced bactericidal, antioxidant, and anticancer properties.

Despite the highly potent biological characteristics, the poor water-solubility of caffeic acid (CA) limits its applications in various domains. Here, we present a facile approach, wherein CA has been treated with dopamine hydrochloride (Dopa.HCl) to obtain a water-soluble acid-base complex, which does not possess any covalent bond between the individual components and thus retains their nativity.

Dysregulation of schizophrenia-associated genes and genome-wide hypomethylation in neurons overexpressing DNMT1.

To study the effects of DNMT1 overexpression on transcript levels of genes dysregulated in schizophrenia and on genome-wide methylation patterns. Transcriptome and DNA methylome comparisons were made between (wild-type) and mouse embryonic stem cells and neurons overexpressing DNMT1. Genes dysregulated in both cells and schizophrenia patients were studied further.

Lactobacillus amylovorus derived lipase-mediated silver derivatization over poly(ε-caprolactone) towards antimicrobial coatings.

Owing to the probiotic origin, lipases-derived from the Lactobacilli sp. are considered to be promising biomaterials for in vivo applications. On a different note, poly(ε-caprolactone) (PCL)-an FDA-approved polymer for implantable applications-lacks inherent antimicrobial property, because of which suitable modifications are required to render it with bactericidal activity.

Functional Analysis of SNPs ( and ) Associated with Schizophrenia.

A recent study showed the association of minor alleles of (T allele) and (T allele) of with schizophrenia (SZ) and suggested their effects on splicing of the transcripts. We performed a replication study using 310 controls and 304 SZ patients and confirmed the association of the homozygous minor allele genotypes with SZ ( = 0.04 for and  = 0.

Analysis of transcript levels of a few schizophrenia candidate genes in neurons from a transgenic mouse embryonic stem cell model overexpressing DNMT1.

Overexpression of DNA Methyltransferase I (DNMT1) is considered as one of the etiological factors for schizophrenia (SZ). However, information on genes subjected to dysregulation because of DNMT1 overexpression is limited. To test whether a larger group of SZ-associated genes are affected, we selected 15 genes reported to be dysregulated in patients (Gad1, Reln, Ank3, Cacna1c, Dkk3, As3mt, Ppp1r11, Smad5, Syn1, Wnt1, Pdgfra, Gsk3b, Cxcl12, Tcf4 and Fez1).

Schrodinger's scat: a critical review of the currently available tiger (Panthera Tigris) and leopard (Panthera pardus) specific primers in India, and a novel leopard specific primer.

Background: Non-invasive sampling has opened avenues for the genetic study of elusive species, which has contributed significantly to their conservation. Where field based identity of non-invasive sample is ambiguous (e.g.