Simultaneous Determination of Anti-diabetic Drugs in Their Combined Dosage Forms Using HPLC: An Experimental Design Approach.

A liquid chromatographic method has been established for the separation of five presently existing anti-diabetic drugs-metformin, pioglitazone, glibenclamide, glimepiride and repaglinide using design of experiments methodology. The main objective of this method is to identify the suitable conditions for the adequate separation of these five drugs with minimal analysis time. Experiment design is one of the Quality by design approaches, which incorporates multifaceted combinations of independent variables and their interactions during the optimization of analytical methods for the determination of drugs.