Publications by authors named "Prakash Babu Adhikari"

Most of the studied MADS box members are linked to flowering and fruit traits. However, higher volumes of studies on type II of the two types so far suggest that the florigenic effect of the gene members could just be the tip of the iceberg. In the current study, we used a systematic approach to obtain a general overview of the MADS box members' cross-trait and multifactor associations, and their pleiotropic potentials, based on a manually curated local reference database.

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Phase transition and floral induction in citrus requires several years of juvenility after germination. Such a long period of juvenility has been a major hindrance to its genetic improvement program. Studies have shown that miR156 along with its downstream genes SQUAMOSA PROMOTER BINDING PROTEINS (SBP) and SBP-LIKE (SPL) mediate the phase transition and floral induction process in plants.

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is a key regulator of the genetic network behind pollen tube attraction toward the female gametophyte. is specifically expressed in the synergid cells (SCs), a female gametophyte component cells specialized for pollen tube attraction. However, it had not been clear how exactly achieves this specific expression pattern.

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Citric acid and anthocyanin contents were co-selected during domestication. Pummelo is a founding species in the genus, but the domestication of pummelo has not been well studied. Here, we compared the citric acid and anthocyanin contents of a low citric acid pummelo ( LCA) and its high citric acid variety (HCA) from the same cultivation area in China.

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Fruit shape is an important trait for fruit appearance and commercial value. Diversity of fruit-shape has been utilized in the breeding of pummelo (Citrus maxima), a basic species in Citrus. However, little is known about genetic basis of fruit shape in citrus.

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Pollen tube (PT) serves as a vehicle that delivers male gametes (sperm cells) to a female gametophyte during double fertilization, which eventually leads to the seed formation. It is one of the fastest elongating structures in plants. Normally, PTs traverse through the extracellular matrix at the transmitting tract after penetrating the stigma.

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Carbohydrates (sugars) are an essential energy-source for all life forms. They take a significant share of our daily consumption and are used for biofuel production as well. However, sugarcane and sugar beet are the only two crop plants which are used to produce sugar in significant amounts.

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Article Synopsis
  • Transgenic orange mint (Mentha × piperita f. citrata) was created by overexpressing the tobacco NtLTP1 gene, leading to increased monoterpene accumulation in glandular trichomes.
  • This genetic manipulation resulted in larger trichome heads, enhancing the plant's ability to emit volatile compounds.
  • Analysis revealed that linalyl acetate was the most prevalent monoterpene, and overall volatile emission was significantly higher in the transgenic plants compared to the wild-type controls.
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Gametophytic mutants share very small proportion of the total mutants generated by any mutagenic approach; even rarer are the fertilization-defective gametophytic mutants. They require an efficient and targeted strategy instead of 'brute force' screening approach. The classical gametophyte mutant screening method, mainly based on the segregation distortion, can distinguish gametophytic mutants from the others.

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Plant reproduction is an extremely important phenomenon, as it is strongly associated with plant genetics and early development. Additionally, foundations of the reproductive system have direct implications on plant breeding and agriculture. Investigation of the functions of male and female gametophytes is critical since their fusion is required for seed formation.

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In Artemisia annua plants, glandular trichomes (GTs) are responsible for the biosynthesis and secretion of sesquiterpene lactones including artemisinin/arteannuin B. Nonspecific lipid transfer proteins (LTPs) in plants bind and carry lipid molecules across the cell membrane and are also known as secretary proteins. Interestingly, the transcripts of LTP genes are exceptionally abundant in the GTs of A.

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Background: Interspecific ginseng hybrid,  ×  () has vigorous growth and produces larger roots than its parents. However, F progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid.

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Article Synopsis
  • The PdFRS gene from Populus davidiana was identified as a monofunctional friedelin synthase, crucial for producing the triterpene friedelin through oxidosqualene cyclase activity.
  • Friedelin, a pentacyclic triterpene, is abundant in various plant tissues, especially in cork from trees, and its synthesis is regulated by the PdFRS gene, which was strongly activated by methyl jasmonate treatments.
  • Heterologous expression of PdFRS in yeast confirmed friedelin production, and transgenic plants overexpressing this gene demonstrated increased friedelin accumulation, showcasing the gene's potential for enhancing triterpene biosynthesis.
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Production of compound K (a ginsenoside saponin) and its precursors in transgenic tobacco resulted in stunted growth and seed set failure, which may be caused by strong autotoxicity of heterologously produced phytochemicals against the tobacco itself. Panax ginseng roots contain various saponins (ginsenosides), which are major bioactive compounds. A monoglucosylated saponin, compound K (20-O-(β-D-glucopyranosyl)-20(S)-protopanaxadiol), has high medicinal and cosmetic values but is present in undetectable amounts in naturally grown ginseng roots.

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Protopanaxadiol (PPD) is an aglycone of dammarene-type ginsenoside and has high medicinal values. In this work, we reported the PPD production in transgenic tobacco co-overexpressing PgDDS and CYP716A47. PPD is an aglycone of ginsenosides produced by Panax species and has a wide range of pharmacological activities.

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