Publications by authors named "Pradyumna A Joshi"
Cellulosic nanomaterials have significantly promoted the development of sensing devices, drug delivery, and bioreactor processes. Their synthetic flexibility makes them a prominent choice for immobilizing biomolecules or cells. In this work, we developed a practical and user-friendly approach to accessing cellulose nanoparticles (CNPs).
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- Post-translational modifications (PTMs) like SUMOylation play a key role in regulating protein functions and are essential for various cellular processes, including signal transduction and stress responses.
- Unlike ubiquitination, SUMOylation can significantly alter a protein's structure, stability, and interactions, highlighting its importance in cellular regulation, and its deregulation is linked to diseases.
- Despite the challenges in detecting SUMOylation due to its low occurrence, bioinformatics and mass spectrometry techniques have been developed to identify and validate SUMO modifications, aiding in the understanding of their biological functions.
Loss of function studies shed significant light on the involvement of a gene or gene product in different cellular processes. Short hairpin RNA (shRNA) mediated RNA interference (RNAi) is a classical yet straightforward technique frequently used to knock down a gene for assessing its function. Similar perturbations in gene expression can be achieved by siRNA, microRNA, or CRISPR-Cas9 methods also.
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