Novel, Rapid, and Simple Isocratic UPLC-UV Method for Estimating Nitrate and Nitrite Contents in Environmental Water Samples Using an Analytical Quality by Design Approach.

In this study, we reported a novel reverse-phase UPLC method for the simultaneous estimation of nitrite and nitrate ions using a Quality by Design (QbD) approach. Nitrite and nitrate ions were separated on an ACQUITY CSH Fluoro-Phenyl column (100 × 2.1 mm, 1.