Anti-CRISPR-Based and CRISPR-Based Genome Editing of Rod-Shaped Virus 2.

Genetic engineering of viruses has generally been challenging. This is also true for archaeal rod-shaped viruses, which carry linear double-stranded DNA genomes with hairpin ends. In this paper, we describe two different genome editing approaches to mutate the rod-shaped virus 2 (SIRV2) using the archaeon LAL14/1 and its derivatives as hosts.