Effect of stanozolol on factors VIII and IX and serum aminotransferases in haemophilia.

The treatment of haemophilia has been dramatically improved since the introduction of factor VIII and IX concentrates, however these concentrates have brought new problems such as hepatitis and A.I.D.

Effects of phenylamide herbicides on the physical properties of phosphatidylcholine membranes.

A number of phenylamide herbicides are observed to uncouple electron transport in isolated chloroplasts and mitochondria and alter the H+ permeability of artificial liposomes. Several of these phenylamides were incorporated into phosphatidylcholine multilamellar and small unilamellar vesicles to measure their effects on the physical properties of membranes. X-ray diffraction analysis of the multilamellar vesicles revealed that the herbicides partitioned into the hydrocarbon chain region of the bilayer, but caused only minimal perturbations on hydrocarbon chain packing.

Alert odor from skin gland in deer.

Black-tailed deer (Odocoileus hemionus columbianus) discharge an odor from the metatarsal (MT) gland, located on the hindleg, when disturbed or alarmed. Freely moving, captive deer were exposed to the MT odor by means of a remote-release apparatus. Responses by males and females to MT odor from both sexes were recorded with a coding system and a video camera.

New structural model for mixed-chain phosphatidylcholine bilayers.

Multilamellar suspensions of a mixed-chain saturated phosphatidylcholine with 18 carbon atoms in the sn-1 chain and 10 carbon atoms in the sn-2 chain have been analyzed by X-ray diffraction techniques. The structural parameters for this lipid in the gel state are quite different than usual phosphatidylcholine bilayer phases. A symmetric and sharp wide-angle reflection at 4.

The effect of beta-adrenoceptor antagonists on the pharmacokinetics and pharmacodynamics of warfarin after a single dose.

The effects of three beta-adrenoceptor antagonists (propranolol, metoprolol and atenolol) on the serum kinetics and pharmacodynamics of warfarin given in a single oral dose (15 mg) were studied in six normal subjects. At the same degree of beta-adrenoceptor blockade, as assessed by the decrease of exercise tachycardia, propranolol increased the area under the serum warfarin concentration time curve (AUC) by 16.3 +/- 14.

Identification of the plasminogen activator secreted by human fibroblasts.

Plasminogen activator was partially purified from cultured human fibroblasts. The enzyme binds to fibrin, has a molecular weight of 68,000 and is antigenically identical to human uterine tissue plasminogen activator.

Chemistry of lipid peroxidation.

The free radical chemistry of lipid peroxidation is complex. The classical mechanism of autoxidation involving a peroxy radical abstracting hydrogen atom from lipid and oxygen addition to the carbon radical thus formed must be modified to include (1) peroxy radical beta fragmentation and (2) peroxy radical cyclization. A host of diene hydroperoxides, cyclic peroxides, bicyclic peroxides and epoxy alcohols may be formed in free fatty acid or phospholipid autoxidation.

Synthesis of mixed-acid phosphatidylcholines and high pressure liquid chromatographic analysis of isomeric lysophosphatidylcholines.

A new method for the synthesis of mixed-chain phosphatidylcholines is reported. Silver ion catalyzed acylation of lysophosphatidylcholines by 2-thiopyridyl esters occurs rapidly (10 min) at room temperature in organic solvents. Yields of isomerically pure mixed-chain phosphatidylcholines (greater than 98% isomeric purity) are generally greater than 80%.

The effects of cyanide on the growth and respiration of Enterobacter aerogenes in continuous culture.

The effect of cyanide on the physiology of lactate- and oxygen-limited Enterobacter aerogenes NCTC 10336 was studied in chemostat culture (D = 0.1 h-1). In the absence of cyanide, the molar growth yield from oxygen (YO2) under oxygen limitation was 60% of the carbon-limited value.

Enzymatic properties of human glucuronyltransferase and a sensitive method for its assay in a stable B lymphocyte cell line.

Properties of lymphocyte glucuronyltransferase were studied in homogenates of SN1006 cells. A sensitive assay procedure for lymphocyte glucuronyltransferase was developed utilizing radioactive testosterone as the acceptor substrate and TLC for separation of the metabolite. The method is capable of detecting picomolar quantities of the product.

Substrate specificity of human UDP-glucuronyltransferase in cultured lymphocytes.

1. This study establishes the presence of UDP-glucuronyltransferase activity for non-steroidal as well as steroidal substrates, in cultured human B-lymphocytes. Glucuronidation of alpha-naphthol and testosterone was demonstrated in homogenates of two cell lines, SN1006 and RPMI-1788, and that of phenolphthalein, 4-methylumbelliferone, p-nitrophenol and estradiol in the cell line with the higher glucuronyltransferase activity, SN1006.

Oxidation of arachidonic acid in micelles by superoxide and hydrogen peroxide.

Arachidonic acid was co-oxidized by xanthine oxidase. Both superoxide radical and hydrogen peroxide were required for oxidation, as shown by essentially complete inhibition caused by superoxide dismutase or by catalase. Pure arachidonate, free of lipid hydroperoxides, was susceptible to this co-oxidation, and the presence of lipid hydroperoxides did not accelerate the process.

Elevation of lysosomal enzymes in primary Lewis lung tumor correlated with the initiation of metastasis.

Lysosomal enzymes were elevated about two-fold in primary s.c. Lewis lung carcinoma as compared with metastatic nodules in the lung.

Arachidonate metabolism via lipoxygenase and 12L-hydroperoxy-5,8,10,14-icosatetraenoic acid peroxidase sensitive to anti-inflammatory drugs.

The enzymes of arachidonate metabolism via the lipoxygenase pathway in human platelet cytosol have been characterized and partially purified. The lipoxygenase activity has a pH optimum of 7.3 and reaches half-maximal activity at an arachidonate concentration of 80 microM.

Elevated beta-thromboglobulin levels and circulating platelet aggregates in diabetic microangiopathy.

beta-Thromboglobulin levels and platelet-aggregate ratios were determined in blood-samples from healthy control subjects and from diabetic patients with and without microangiopathic complications. Patients with diabetic microangiopathy had significantly elevated beta-thromboglobulin levels and also reversible platelet aggregates. In nine newly treated diabetics blood-glucose control was associated with a significant fall in plasma beta-thromboglobulin.

Platelet aggregation in patients with peripheral vascular disease.

Platelet aggregation has been studied in 28 control subjects and 46 patients with peripheral vascular disease. The platelets from the patients with peripheral vascular disease were significantly more sensitive to the aggregating effects of adenosine diphosphate and adrenaline than those from the control group (P less than 0.001).