Barakol-induced apoptosis in P19 cells through generation of reactive oxygen species and activation of caspase-9.

Ethnopharmacological Relevance: Barakol, an anxiolytic agent isolated from Senna siamea leaves which has been traditionally used for producing natural sleep, has been described as toxic to patients.

Aim Of The Study: The aim of current study was to investigate the molecular mechanism of barakol-induced toxicity in mouse embryonal carcinoma P19 cell model.

Materials And Methods: XTT assay was used to determine cell viability in P19 cells treated with barakol.

Application of flow cytometric beads for simultaneous CD4 and CD8 determinations in HIV-1 infected thalassemia patients.

Background: CD4 and CD8 are determined by either a dual step assay using a calculation of absolute lymphocytes obtained from routine CBC or by a single step assay by including cytometric beads and fluorescent microspheres in the sample so that an absolute cell count can be made, simultaneously. Since thalassemia is common in Thailand, and a number of nucleated red blood cells (NRBC) are observed in peripheral blood of thalassemia patients with severe anemia, we speculated that NRBC in HIV-1 infected thalassemia patient with severe anemia might cause an error in CD4 and CD8 determinations in the dual step assay.

Objective: Comparing cytometric beads in three-color-lyse-no-wash in the single step assay in CD4 and CD8 with dual step assay by calculation using absolute lymphocytes obtained from routine CBC in HIV-1 infected thalassemia patients with severe anemia.

Stromal cell-derived factor (SDF) 1-3'A polymorphism may play a role in resistance to HIV-1 infection in seronegative high-risk Thais.

Objective: It is known that a 32-bp-deleted CCR5 mutant (CCR5-Delta32) plays a critical role in resistance to human immunodeficiency virus type 1 (HIV-1) infection. We became aware of a number of seronegative patients at high risk of HIV-1 infections. Since CCR-Delta32 is not found in Thais, we examined the role of stromal cell-derived factor (SDF) 1-3'A polymorphism in resistance to HIV-1 in seronegative Thai prostitutes at high risk.

Cloning and sequencing of ETV6/RUNX1 (TEL/AML1) variant in acute lymphoblastic leukemia.

The most common gene fusion (up to 25%) in childhood acute lymphoblastic leukemia (ALL) is that between ETV6 and RUNX1 (previously TEL and AML1, respectively; we here use the old nomenclature, for ease of reference to the literature). We determined the incidence of TEL/AML1 translocation with reverse transcriptase polymerase chain reaction (RT-PCR) and flow-cytometric immunophenotyping of newly diagnosed pediatric acute lymphoblastic leukemia patients in Thailand. The TEL/AML1 fusion genes were cloned into plasmids and sequenced.

Stromal cell-derived factor (SDF) 1-3'A polymorphism and sequences in Thais.

Stromal cell derived factor (SDF) 1-3'A polymorphism in Thai subjects was determined by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products using a restriction enzyme Msp 1 cleavage. The allelic frequency of SDF1-3'A in this population was 0.289.